Restricted Presence of POU6F2 in Human Corneal Endothelial Cells Uncovered by Extension of the Promoter-level Expression Atlas

DOI: 10.1016/j.ebiom.2017.10.024

Authors: Masahito Yoshihara; Susumu Hara; Motokazu Tsujikawa; Satoshi Kawasaki; Yoshihide Hayashizaki; Masayoshi Itoh; Hideya Kawaji; Kohji Nishida

Step 1: endothelial 1

day 0 ~ 28

culture “adherent” human HCEP in “DMEM” for “28” days. For ECM, use “FNC coating mix” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “28” days.

Ideally, “EC” should express “POU6F2”, “TFAP2B”, “LMX1B”, “PITX2”, “COL4A3”, “COL4A4” at the end of culture.