Characterization of the proteome and metabolome of human liver sinusoidal endothelial-like cells derived from induced pluripotent stem cells

DOI: 10.1016/j.diff.2021.06.001

Authors: Mathieu Danoy; Rachid Jellali; Yannick Tauran; Johanna Bruce; Marjorie Leduc; Françoise Gilard; Bertrand Gakière; Benedikt Scheidecker; Taketomo Kido; Atsushi Miyajima; Fabrice Soncin; Yasuyuki Sakai; Eric Leclerc

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” human PSC in “unspecified medium”.

Step 2: endothelial 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

culture “unspecified method” human PSC in “unspecified medium”.

Ideally, “EC” should express “APLN”, “LYVE1”, “VWF”, “ESAM”, “ANGPT2”, “PECAM1”, “CDH13”, “THBS1”, “ICAM1”, “CDH5”, “CLDN5”, “ITGA5”, “ITGA3”, “ENG”, “ESM1”, “MCAM”, “CD93”, “MMRN2”, “CAV1”, “NOSTRIN”, “ICAM2”, “STAB1”, “CD36”, “ACE”, “NRP2”, “PLVAP”, “ERG”, “MRC1”, “CLEC14A”, “AQP1”, “NOS3”, “KDR”, “STAB2” at the end of culture.