Assessment of iPSC teratogenicity throughout directed differentiation toward an alveolar-like phenotype

DOI: 10.1016/j.diff.2019.01.003

Authors: Adam Mitchell; Heather Wanczyk; Todd Jensen; Christine Finck

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “StemMACS iPS-Brew XF”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table.

Ideally, “PSC” should be obtained at the end of culture.