Cell-type-specific regulation of APOE and CLU levels in human neurons by the Alzheimer’s disease risk gene SORL1

DOI: 10.1016/j.celrep.2023.112994

Authors: Hyo Lee; Aimee J. Aylward; Richard V. Pearse; Alexandra M. Lish; Yi-Chen Hsieh; Zachary M. Augur; Courtney R. Benoit; Vicky Chou; Allison Knupp; Cheryl Pan; Srilakshmi Goberdhan; Duc M. Duong; Nicholas T. Seyfried; David A. Bennett; Mariko F. Taga; Kevin Huynh; Matthias Arnold; Peter J. Meikle; Philip L. De Jager; Vilas Menon; Jessica E. Young; Tracy L. Young-Pearse

Step 1: iPSC/ESC maintainance

day 0 ~ 2

culture “adherent” human PSC in “StemFlex Medium” for “2” days. “PSC” should be “downregulated” for “SORL1”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should be changed once in “2” days.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: endothelial 1

day 2 ~ 3

culture “adherent” human PSC in “E6 medium” for “1” days. “PSC” should be “downregulated” for “SORL1”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should be changed once in “1” days.

Ideally, “EC” should be obtained at the end of culture.

Step 3: endothelial 2

day 3 ~ 4

culture “adherent” human PSC in “hESFM” for “1” days. “PSC” should be “downregulated” for “SORL1”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Ideally, “EC” should be obtained at the end of culture.