A single-cell level comparison of human inner ear organoids with the human cochlea and vestibular organs

DOI: 10.1016/j.celrep.2023.112623

Authors: Wouter H. van der Valk; Edward S.A. van Beelen; Matthew R. Steinhart; Carl Nist-Lund; Daniel Osorio; John C.M.J. de Groot; Liang Sun; Peter Paul G. van Benthem; Karl R. Koehler; Heiko Locher

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “E8” for “1” days. For ECM, use “vitronectin” at “5.0e-7” “g/m”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: Primitive streak 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

culture “spheroid” human PSC in “unspecified medium”. Medium should contain factors shown in the following table.

Step 3: endothelial 1

day 2 ~ 3 (duration missing in database; assumed 1 day)

culture “spheroid” human PSC in “unspecified medium”.

Ideally, “EC” should express “PECAM1” at the end of culture.