Assembly and Function of a Bioengineered Human Liver for Transplantation Generated Solely from Induced Pluripotent Stem Cells

DOI: 10.1016/j.celrep.2020.107711

Authors: Kazuki Takeishi; Alexandra Collin de l’Hortet; Yang Wang; Kan Handa; Jorge Guzman-Lepe; Kentaro Matsubara; Kazutoyo Morita; Sae Jang; Nils Haep; Rodrigo M. Florentino; Fangchao Yuan; Ken Fukumitsu; Kimimasa Tobita; Wendell Sun; Jonathan Franks; Evan R. Delgado; Erik M. Shapiro; Nicolas A. Fraunhoffer; Andrew W. Duncan; Hiroshi Yagi; Tomoji Mashimo; Ira J. Fox; Alejandro Soto-Gutierrez

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” unknown species cell and human PSC in “mTeSR” for “1” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should be changed once in “1” days.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: endothelial 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

culture “unspecified method” unknown species cell and human PSC in “unspecified medium”.

Ideally, “EC” should express “PECAM1”, “NOS3”, “VWF” at the end of culture.