Generation of Lungs by Blastocyst Complementation in Apneumic Fgf10-Deficient Mice

DOI: 10.1016/j.celrep.2020.107626

Authors: Akihiko Kitahara; Qingsong Ran; Kanako Oda; Akihiro Yasue; Manabu Abe; Xulu Ye; Toshikuni Sasaoka; Masanori Tsuchida; Kenji Sakimura; Yoichi Ajioka; Yasuo Saijo; Qiliang Zhou

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” mouse ESC in “Knockout DMEM”. Medium should contain factors shown in the following table.

Ideally, “ESC” should express “GFP” at the end of culture.