Specification and Diversification of Pericytes and Smooth Muscle Cells from Mesenchymoangioblasts

DOI: 10.1016/j.celrep.2017.05.019

Authors: Akhilesh Kumar; Saritha Sandra D’Souza; Oleg V. Moskvin; Huishi Toh; Bowen Wang; Jue Zhang; Scott Swanson; Lian-Wang Guo; James A. Thomson; Igor I. Slukvin

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “unspecified medium”. For ECM, use “mouse embryonic fibroblasts” at “N/A” “N/A”.

Step 2: mesoderm 1

day 1 ~ 3

culture “adherent” human PSC in “unspecified medium” for “2” days. For ECM, use “OP9 stromal cells” at “N/A” “N/A”. Medium should be changed once in “2” days.

Ideally, “mesoderm” should express “APLNR”, “PDGFRA”, “KDR”, “TSF21”, “FOXF1”, “HAND2”, “NKX-2.5”, “DLK1”, “HAND1”, “TBR1”, “LHX”, “EMCN” at the end of culture.

Step 3: mesoderm 2

day 3 ~ 6

culture “unspecified method” human PSC in “unspecified medium” for “3” days. Medium should contain factors shown in the following table, and should be changed once in “3” days.

Ideally, “EC” should express “CDH5”, “PECAM1” at the end of culture. Ideally, “mesoderm” should express “MCAM”, “NGFR”, “PDGFRB” at the end of culture.