Identification of the Hemogenic Endothelial Progenitor and Its Direct Precursor in Human Pluripotent Stem Cell Differentiation Cultures

DOI: 10.1016/j.celrep.2012.08.002

Authors: Kyung-Dal Choi; Maxim A. Vodyanik; Padma Priya Togarrati; Kran Suknuntha; Akhilesh Kumar; Fnu Samarjeet; Mitchell D. Probasco; Shulan Tian; Ron Stewart; James A. Thomson; Igor I. Slukvin

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “unspecified medium”.

Step 2: mesoderm 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “unspecified medium”.

Ideally, “mesoderm” should express “APLNR”, “PDGFRA”, “KDR” at the end of culture.

Step 3: mesoderm 2

day 2 ~ 3 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “unspecified medium”.

Ideally, “mesoderm” should express “KDR”, “APLNR”, “PDGFRA”, “TEK”, “CD34”, “CD201”, “MCAM” at the end of culture.

Step 4: endothelial 1

day 3 ~ 4 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “Endothelial Serum-Free Medium”. For ECM, use “FN1” at “N/A” “N/A”. Medium should contain factors shown in the following table.

Ideally, “EC” should express “CDH5”, “PECAM1”, “KDR”, “CD34”, “CD201”, “ESAM”, “MCAM” at the end of culture.