Human embryonic stem cell-derived CD34+ cells function as MSC progenitor cells

DOI: 10.1016/j.bone.2010.06.020

Authors: Ross A. Kopher; Vesselin R. Penchev; Mohammad S. Islam; Katherine L. Hill; Sundeep Khosla; Dan S. Kaufman

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human ESC in “DMEM/F12”. Medium should contain factors shown in the following table.

Ideally, “ESC” should be obtained at the end of culture.

Step 2: mesoderm 1

day 1 ~ 3

culture “adherent” human ESC in “RPMI” for “2” days. For ECM, use “Gelatin” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Step 3: endothelial 1

day 3 ~ 5

culture “adherent” human ESC in “EBM-2” for “2” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “2” days.