On-chip real-time impedance monitoring of hiPSC-derived and artificial basement membrane-supported endothelium

DOI: 10.1016/j.bios.2023.115324

Authors: Xiaochen Huang; Feng Liang; Boxin Huang; Haoyue Luo; Jian Shi; Li Wang; Juan Peng; Yong Chen

Step 1: iPSC/ESC maintainance

day 0 ~ 2

culture “adherent” human PSC in “E8” for “2” days. For ECM, use “vitronectin” at “1” “%”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Step 2: mesoderm 1

day 2 ~ 4

culture “adherent” human PSC in “DMEM/F12” for “2” days. For ECM, use “vitronectin” at “1” “%”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Step 3: endothelial 1

day 4 ~ 6

culture “adherent” human PSC in “human endothelial serum-free medium” for “2” days. For ECM, use “vitronectin” at “1” “%”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Step 4: endothelial 2

day 6 ~ 14

culture “device” human PSC in “human endothelial serum-free medium” for “8” days. For ECM, use “ABM” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “8” days.

Ideally, “EC” should express “PECAM1”, “CDH5”, “Glut1”, “VWF”, “MKI67”, “actin” at the end of culture.