Inhibition of angiogenesis by the secretome from iPSC-derived retinal ganglion cells with Leber’s hereditary optic neuropathy-like phenotypes

DOI: 10.1016/j.biopha.2024.117270

Authors: Shih-Yuan Peng; Chih-Ying Chen; Hsin Chen; Yi-Ping Yang; Mong-Lien Wang; Fu-Ting Tsai; Chian-Shiu Chien; Pei-Yu Weng; En-Tung Tsai; I-Chieh Wang; Chih-Chien Hsu; Tai-Chi Lin; De-Kuang Hwang; Shih-Jen Chen; Shih-Hwa Chiou; Chuan-Chin Chiao; Yueh Chien

Step 1: iPSC/ESC maintainance

day 0 ~ 5

culture “adherent” human PSC in “StemFlex medium” for “5” days. For ECM, use “Geltrex” at “N/A” “N/A”. Medium should be changed once in “5” days.

Ideally, “PSC” should be obtained at the end of culture.