VEGF – Supplemented extracellular matrix is sufficient to induce endothelial differentiation of human iPSC

DOI: 10.1016/j.biomaterials.2019.119283

Authors: Imran Ullah; Raed Abu-Dawud; Jonas Felix Busch; Anja Rabien; Bettina Erguen; Iris Fischer; Petra Reinke; Andreas Kurtz

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” human PSC in “E8”. For ECM, use “kidney dECM” at “N/A” “N/A”. Medium should contain factors shown in the following table.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: mesoderm 1

day 1 ~ 4

culture “unspecified method” human PSC in “APEL2 medium” for “3” days. For ECM, use “kidney dECM” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “3” days.

Ideally, “mesoderm” should be obtained at the end of culture.

Step 3: endothelial 1

day 4 ~ 11

culture “unspecified method” human PSC in “APEL2 medium” for “7” days. For ECM, use “kidney dECM” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “7” days.

Ideally, “EC” should express “VE-CADHERIN”, “PECAM1” at the end of culture.