A synthetic substrate to support early mesodermal differentiation of human embryonic stem cells

DOI: 10.1016/j.biomaterials.2011.07.052

Authors: Yang Liu; Xintong Wang; Dan S. Kaufman; Wei Shen

Step 1: mesoderm 1

day 0 ~ 2

culture “adherent” unknown species cell and human ESC in “unspecified medium” for “2” days. For ECM, use “substrate functionalized with Lα5β1 and Lα6β1” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Ideally, “mesoderm” should express “TBXT”, “MIXL1”, “Wnt3” at the end of culture.

Step 2: endothelial 1

day 2 ~ 5

culture “adherent” unknown species cell and human ESC in “unspecified medium” for “3” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “3” days.

Step 3: endothelial 2

day 5 ~ 13

culture “adherent” unknown species cell and human ESC in “unspecified medium” for “8” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “8” days.

Ideally, “EC” should express “CD34” at the end of culture.