Combinational regenerative inductive effect of bio-adhesive hybrid hydrogels conjugated with hiPSC-derived myofibers and its derived EVs for volumetric muscle regeneration

DOI: 10.1016/j.bioactmat.2024.09.013

Authors: Jiseong Kim; Myung Chul Lee; Jieun Jeon; Alejandra Rodríguez-delaRosa; Yori Endo; Da-Seul Kim; Andrea Donaxi Madrigal-Salazar; Jeong Wook Seo; Hyeseon Lee; Ki-Tae Kim; Jae-I Moon; Seung Gwa Park; Mariana Carolina Lopez-Pacheco; Abdulhameed F. Alkhateeb; Nebras Sobahi; Nicole Bassous; Wenpeng Liu; Jae Seo Lee; Seongsoo Kim; Dilara Yilmaz Aykut; Mahmoud Lotfi Nasr; Mohammad Asif Hussain; Soo-Hong Lee; Woo-Jin Kim; Olivier Pourquié; Indranil Sinha; Su Ryon Shin

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “Matrigel” at “N/A” “N/A”.

Ideally, “PSC” should be obtained at the end of culture.