Co-culture of human induced pluripotent stem cell-derived retinal pigment epithelial cells and endothelial cells on double collagen-coated honeycomb films

DOI: 10.1016/j.actbio.2019.11.002

Authors: Maria Teresa Calejo; Jaakko Saari; Hanna Vuorenpää; Elina Vuorimaa-Laukkanen; Pasi Kallio; Katriina Aalto-Setälä; Susanna Miettinen; Heli Skottman; Minna Kellomäki; Kati Juuti-Uusitalo

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” human PSC in “E8”.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: endothelial 1

day 1 ~ 3

culture “adherent” human PSC in “unspecified medium” for “2” days. For ECM, use “Gelatin” at “0.1” “%”. Medium should be changed once in “2” days.

Ideally, “EC” should express “VWF”, “PECAM1”, “CDH5”, “CD34” at the end of culture.