Transcriptome profiling of 3D co-cultured cardiomyocytes and endothelial cells under oxidative stress using a photocrosslinkable hydrogel system

DOI: 10.1016/j.actbio.2017.06.031

Authors: Xiaoshan Yue; Aylin Acun; Pinar Zorlutuna

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “mTeSR” for “1” days. For ECM, use “Geltrex” at “N/A” “N/A”. Medium should be changed once in “1” days.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: endothelial 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

culture “unspecified method” unknown species cell in “unspecified medium”.

Ideally, “EC” should be obtained at the end of culture.