Engineered myocardium model to study the roles of HIF-1α and HIF1A-AS1 in paracrine-only signaling under pathological level oxidative stress

DOI: 10.1016/j.actbio.2017.06.023

Authors: Aylin Acun; Pinar Zorlutuna

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “Geltrex” at “N/A” “N/A”.

Step 2: mesoderm 1

day 1 ~ 4

culture “adherent” human PSC in “N2B27 medium” for “3” days. For ECM, use “Geltrex” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “3” days.

Step 3: endothelial 1

day 4 ~ 7

culture “adherent” human PSC in “StemPro” for “3” days. For ECM, use “Geltrex” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “3” days.

Step 4: endothelial 2

day 7 ~ 8 (duration missing in database; assumed 1 day)

culture “adherent” human EC in “EGM2”. For ECM, use “FN1” at “N/A” “N/A”.

Ideally, “EC” should express “PECAM1”, “EDN1”, “VWF”, “VE-CAD” at the end of culture.