Combinatorial extracellular matrix microenvironments promote survival and phenotype of human induced pluripotent stem cell-derived endothelial cells in hypoxia
DOI: 10.1016/j.actbio.2016.08.003
Authors: Luqia Hou; John Coller; Vanita Natu; Trevor J. Hastie; Ngan F. Huang
Step 1: endothelial 1
day 0 ~ 14
culture “unspecified method” human PSC in “unspecified medium” for “14” days. Medium should contain factors shown in the following table, and should be changed once in “14” days.
| concentration | unit | |
|---|---|---|
| VEGF | — | — |
| BMP4 | — | — |
Ideally, “EC” should express “PECAM1”, “VWF” at the end of culture.