A matrix micropatterning platform for cell localization and stem cell fate determination
DOI: 10.1016/j.actbio.2010.06.033
Authors: Ngan F. Huang; Bhagat Patlolla; Oscar Abilez; Himanshu Sharma; Jaykumar Rajadas; Ramin E. Beygui; Christopher K. Zarins; John P. Cooke
Step 1: iPSC/ESC maintainance
day 0 ~ 1 (duration missing in database; assumed 1 day)
culture “adherent” mouse ESC in “DMEM”. For ECM, use “Gelatin” at “N/A” “N/A”. Medium should contain factors shown in the following table.
| concentration | unit | |
|---|---|---|
| FBS | 15 | % |
| PSA | 1 | % |
| NEAA | 1.0e-4 | mol/l |
| 2-mercaptoethanol | 1.0e-4 | mol/l |
| LIF | 1.0e+6 | U/l |
Ideally, “ESC” should express “CDH1”, “alkaline phosphatase” at the end of culture.