Cyclic Strain Promotes H19 Expression and Vascular Tube Formation in iPSC-Derived Endothelial Cells

DOI: 10.1007/s12195-020-00617-0

Authors: Mark J. Vander Roest; W. David Merryman

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “mTeSR” for “1” days. For ECM, use “Matrigel” at “0.1” “g/m2”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: mesoderm 1

day 1 ~ 4

culture “device” human PSC in “N2B27 medium” for “3” days. For ECM, use “Matrigel” at “0.1” “g/m2”. Medium should contain factors shown in the following table, and should be changed once in “3” days.

Ideally, “mesoderm” should be obtained at the end of culture.

Step 3: endothelial 1

day 4 ~ 5

culture “adherent” human PSC in “StemPro” for “1” days. For ECM, use “Matrigel” at “0.1” “g/m2”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Ideally, “EC” should be obtained at the end of culture.