VSELs Maintain their Pluripotency and Competence to Differentiate after Enhanced Ex Vivo Expansion

DOI: 10.1007/s12015-018-9821-1

Authors: Rachid Lahlil; Maurice Scrofani; Romain Barbet; Céline Tancredi; Anne Aries; Philippe Hénon

Step 1: mesoderm 1

day 0 ~ 2

culture “adherent” unknown species cell and human VSEL in “unspecified medium” for “2” days. For ECM, use “fibronectin/gelatin” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Ideally, “mesoderm” should express “PDGFRa”, “KDR”, “MCAM”, “CD105”, “CD34”, “PTPRC” at the end of culture.