Optimization of Synthetic mRNA for Highly Efficient Translation and its Application in the Generation of Endothelial and Hematopoietic Cells from Human and Primate Pluripotent Stem Cells

DOI: 10.1007/s12015-018-9805-1

Authors: Kran Suknuntha; Lihong Tao; Vera Brok-Volchanskaya; Saritha S. D’Souza; Akhilesh Kumar; Igor Slukvin

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “E8”. For ECM, use “vitronectin” at “N/A” “N/A”.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: endothelial 1

day 1 ~ 2

culture “adherent” human PSC in “E8” for “1” days. “PSC” should be “upregulated” for “ETV2”. For ECM, use “vitronectin” at “N/A” “N/A”. Medium should be changed once in “1” days.

Ideally, “EC” should express “PECAM1”, “CDH5” at the end of culture.

Step 3: endothelial 2

day 2 ~ 3 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “Endothelial cell growth medium”. “PSC” should be “upregulated” for “ETV2”. For ECM, use “Collagen” at “N/A” “N/A”.

Ideally, “EC” should express “PECAM1”, “CDH5” at the end of culture.