Human Vascular Tissue Models Formed from Human Induced Pluripotent Stem Cell Derived Endothelial Cells

DOI: 10.1007/s12015-014-9549-5

Authors: David G. Belair; Jordan A. Whisler; Jorge Valdez; Jeremy Velazquez; James A. Molenda; Vernella Vickerman; Rachel Lewis; Christine Daigh; Tyler D. Hansen; David A. Mann; James A. Thomson; Linda G. Griffith; Roger D. Kamm; Michael P. Schwartz; William L. Murphy

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” human PSC in “unspecified medium”.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: endothelial 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

culture “unspecified method” unknown species cell in “unspecified medium”.

Ideally, “EC” should express “PECAM1”, “ENG”, “CDH5”, “KDR”, “VWF”, “ICAM1”, “MCAM”, “ZO-1” at the end of culture.