Porcine ovarian cortex-derived putative stem cells can differentiate into endothelial cells in vitro

DOI: 10.1007/s00418-021-02016-6

Authors: Kamil Wartalski; Gabriela Gorczyca; Jerzy Wiater; Zbigniew Tabarowski; Małgorzata Duda

Step 1: iPSC/ESC maintainance

day 0 ~ 2

culture “adherent” unknown species cell and pig PSC in “DMEM/F12” for “2” days. For ECM, use “poly-L-lysine” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Ideally, “PSC” should express “ITGB1”, “THY1”, “ENG”, “CDH5”, “KDR” at the end of culture.

Step 2: endothelial 1

day 2 ~ 4

culture “adherent” unknown species cell and pig PSC in “EGM2” for “2” days. For ECM, use “poly-L-lysine” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Ideally, “EC” should express “CDH5”, “KDR” at the end of culture.