Effective endothelial cell and human pluripotent stem cell interactions generate functional insulin-producing beta cells

DOI: 10.1007/s00125-016-4078-1

Authors: Dodanim Talavera-Adame; Orison O. Woolcott; Joseph Ignatius-Irudayam; Vaithilingaraja Arumugaswami; David H. Geller; Donald C. Dafoe

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” human PSC in “mTeSR”. Medium should contain factors shown in the following table.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: Primitive streak 1

day 1 ~ 2

culture “adherent” unknown species cell and human PSC in “RPMI 1640” for “1” days. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Step 3: Primitive streak 2

day 2 ~ 4

culture “adherent” unknown species cell and human PSC in “RPMI 1640” for “2” days. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Ideally, “DE” should be obtained at the end of culture.