Endothelial Differentiation Protocols

A combined hiPSC-derived endothelial cell and in vitro microfluidic platform for assessing biomaterial-based angiogenesis

Mon, 01 Jan 0001 00:00:00 +0000

A combined hiPSC-derived endothelial cell and in vitro microfluidic platform for assessing biomaterial-based angiogenesis

DOI: 10.1016/j.biomaterials.2018.11.032

Authors: Sylvia L. Natividad-Diaz; Shane Browne; Amit K. Jha; Zhen Ma; Samir Hossainy; Yosuke K. Kurokawa; Steven C. George; Kevin E. Healy

Step 1: iPSC/ESC maintainance

day 0 ~ 2

culture “adherent” human PSC in “E8” for “2” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

A comprehensive library of human transcription factors for cell fate engineering

Mon, 01 Jan 0001 00:00:00 +0000

A comprehensive library of human transcription factors for cell fate engineering

DOI: 10.1038/s41587-020-0742-6

Authors: Alex H. M. Ng; Parastoo Khoshakhlagh; Jesus Eduardo Rojo Arias; Giovanni Pasquini; Kai Wang; Anka Swiersy; Seth L. Shipman; Evan Appleton; Kiavash Kiaee; Richie E. Kohman; Andyna Vernet; Matthew Dysart; Kathleen Leeper; Wren Saylor; Jeremy Y. Huang; Amanda Graveline; Jussi Taipale; David E. Hill; Marc Vidal; Juan M. Melero-Martin; Volker Busskamp; George M. Church

Step 1: iPSC/ESC maintainance

day 0 ~ 1

A first-in-human clinical study of an allogenic iPSC-derived corneal endothelial cell substitute transplantation for bullous keratopathy

Mon, 01 Jan 0001 00:00:00 +0000

A first-in-human clinical study of an allogenic iPSC-derived corneal endothelial cell substitute transplantation for bullous keratopathy

DOI: 10.1016/j.xcrm.2024.101847

Authors: Masatoshi Hirayama; Shin Hatou; Masaki Nomura; Risa Hokama; Osama Ibrahim Hirayama; Emi Inagaki; Kumi Aso; Tomoko Sayano; Hiromi Dohi; Tadaaki Hanatani; Naoko Takasu; Hideyuki Okano; Kazuno Negishi; Shigeto Shimmura

Step 1: iPSC/ESC maintainance

day 0 ~ 21

culture “adherent” human PSC in “StemFit AK03N” for “21” days. For ECM, use “iMatrix-511MG” at “N/A” “N/A”. Medium should be changed once in “21” days.

A Genome-wide Analysis of Human Pluripotent Stem Cell-Derived Endothelial Cells in 2D or 3D Culture

Mon, 01 Jan 0001 00:00:00 +0000

A Genome-wide Analysis of Human Pluripotent Stem Cell-Derived Endothelial Cells in 2D or 3D Culture

DOI: 10.1016/j.stemcr.2017.02.014

Authors: Jue Zhang; Michael P. Schwartz; Zhonggang Hou; Yongsheng Bai; Hamisha Ardalani; Scott Swanson; John Steill; Victor Ruotti; Angela Elwell; Bao Kim Nguyen; Jennifer Bolin; Ron Stewart; James A. Thomson; William L. Murphy

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “E8”. For ECM, use “vitronectin” at “N/A” “N/A”.

A Human Pluripotent Stem Cell-Based Screen for Smooth Muscle Cell Differentiation and Maturation Identifies Inhibitors of Intimal Hyperplasia

Mon, 01 Jan 0001 00:00:00 +0000

A Human Pluripotent Stem Cell-Based Screen for Smooth Muscle Cell Differentiation and Maturation Identifies Inhibitors of Intimal Hyperplasia

DOI: 10.1016/j.stemcr.2019.04.013

Authors: Jue Zhang; Brian E. McIntosh; Bowen Wang; Matthew E. Brown; Mitchell D. Probasco; Sarah Webster; Bret Duffin; Ying Zhou; Lian-Wang Guo; William J. Burlingham; Craig Kent; Michael Ferris; James A. Thomson

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “E8”. For ECM, use “Matrigel” at “N/A” “N/A”.

A human pluripotent stem cell-derived in vitro model of the blood–brain barrier in cerebral malaria

Mon, 01 Jan 0001 00:00:00 +0000

A human pluripotent stem cell-derived in vitro model of the blood–brain barrier in cerebral malaria

DOI: 10.1186/s12987-024-00541-9

Authors: Adnan Gopinadhan; Jason M. Hughes; Andrea L. Conroy; Chandy C. John; Scott G. Canfield; Dibyadyuti Datta

Step 1: iPSC/ESC maintainance

day 0 ~ 3

culture “adherent” human PSC in “mTeSR” for “3” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should be changed once in “3” days.

Ideally, “PSC” should be obtained at the end of culture.

A human VE-cadherin-tdTomato and CD43-green fluorescent protein dual reporter cell line for study endothelial to hematopoietic transition

Mon, 01 Jan 0001 00:00:00 +0000

A human VE-cadherin-tdTomato and CD43-green fluorescent protein dual reporter cell line for study endothelial to hematopoietic transition

DOI: 10.1016/j.scr.2016.09.004

Authors: Ho Sun Jung; Gene Uenishi; Akhilesh Kumar; Mi Ae Park; Matt Raymond; Dustin Fink; Ethan McLeod; Igor Slukvin

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human ESC in “E8”. For ECM, use “vitronectin” at “N/A” “N/A”. Medium should contain factors shown in the following table.

A matrix micropatterning platform for cell localization and stem cell fate determination

Mon, 01 Jan 0001 00:00:00 +0000

A matrix micropatterning platform for cell localization and stem cell fate determination

DOI: 10.1016/j.actbio.2010.06.033

Authors: Ngan F. Huang; Bhagat Patlolla; Oscar Abilez; Himanshu Sharma; Jaykumar Rajadas; Ramin E. Beygui; Christopher K. Zarins; John P. Cooke

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” mouse ESC in “DMEM”. For ECM, use “Gelatin” at “N/A” “N/A”. Medium should contain factors shown in the following table.

A microparticle approach to morphogen delivery within pluripotent stem cell aggregates

Mon, 01 Jan 0001 00:00:00 +0000

A microparticle approach to morphogen delivery within pluripotent stem cell aggregates

DOI: 10.1016/j.biomaterials.2013.05.079

Authors: Andrés M. Bratt-Leal; Anh H. Nguyen; Katy A. Hammersmith; Ankur Singh; Todd C. McDevitt

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” mouse ESC in “N2B27” for “1” days. For ECM, use “Gelatin” at “0.5” “%”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

	concentration	unit
N2	—	—
B27	—	—
BSA	0.05	g/l
PSA	—	—
streptomycin	—	—
amphotericin	—	—
Glutamine	0.002	mol/l

Ideally, “ESC” should be obtained at the end of culture.

A multi-cellular 3D bioprinting approach for vascularized heart tissue engineering based on HUVECs and iPSC-derived cardiomyocytes

Mon, 01 Jan 0001 00:00:00 +0000

A multi-cellular 3D bioprinting approach for vascularized heart tissue engineering based on HUVECs and iPSC-derived cardiomyocytes

DOI: 10.1038/s41598-018-31848-x

Authors: Fabio Maiullari; Marco Costantini; Marika Milan; Valentina Pace; Maila Chirivì; Silvia Maiullari; Alberto Rainer; Denisa Baci; Hany El-Sayed Marei; Dror Seliktar; Cesare Gargioli; Claudia Bearzi; Roberto Rizzi

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” mouse PSC in “KnockOut DMEM” for “1” days. For ECM, use “Fibroblast” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

A Multi-Lineage Screen Reveals mTORC1 Inhibition Enhances Human Pluripotent Stem Cell Mesendoderm and Blood Progenitor Production

Mon, 01 Jan 0001 00:00:00 +0000

A Multi-Lineage Screen Reveals mTORC1 Inhibition Enhances Human Pluripotent Stem Cell Mesendoderm and Blood Progenitor Production

DOI: 10.1016/j.stemcr.2016.04.003

Authors: Emanuel Joseph Paul Nazareth; Nafees Rahman; Ting Yin; Peter William Zandstra

Step 1: iPSC/ESC maintainance

day 0 ~ 2

culture “adherent” human PS in “NutriStem” for “2” days. For ECM, use “Geltrex” at “2” “%”. Medium should be changed once in “2” days.

Ideally, “PS” should be obtained at the end of culture.

A multilayered nanofibrous patch functionalized with adipose tissue extract for the treatment of bladder regeneration

Mon, 01 Jan 0001 00:00:00 +0000

A multilayered nanofibrous patch functionalized with adipose tissue extract for the treatment of bladder regeneration

DOI: 10.1016/j.matdes.2022.110821

Authors: Jing Wang; Chunxiang Feng; Yunpeng Zhu; Zhixian Wang; Xiang Ren; Xing Li; Yisheng Ying; Yiqun Tian; Kai Yu; Shiliang Liu; Chang Liu; Xiaoyong Zeng

No steps available

A New Long Noncoding RNA ALB Regulates Autophagy by Enhancing the Transformation of LC3BI to LC3BII during Human Lens Development

Mon, 01 Jan 0001 00:00:00 +0000

A New Long Noncoding RNA ALB Regulates Autophagy by Enhancing the Transformation of LC3BI to LC3BII during Human Lens Development

DOI: 10.1016/j.omtn.2017.09.011

Authors: Qiuli Fu; Zhenwei Qin; Lifang Zhang; Danni Lyu; Qiaomei Tang; Houfa Yin; Zhijian Chen; Ke Yao

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “Matrigel” at “N/A” “N/A”.

Ideally, “PSC” should be obtained at the end of culture.

A novel efficient strategy to generate liver sinusoidal endothelial cells from human pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

A novel efficient strategy to generate liver sinusoidal endothelial cells from human pluripotent stem cells

DOI: 10.1038/s41598-024-64195-1

Authors: Shang-Ping Tian; Jian-Yun Ge; Yu-Mu Song; Xiao-Qing Yu; Wen-Hao Chen; Yu-Ying Chen; Di Ye; Yun-Wen Zheng

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “StemFit Basic04 medium”. For ECM, use “Matrix511-silk” at “5.0e-6” “l/m2”. Medium should contain factors shown in the following table.

A novel hiPSC-derived system for hematoendothelial and myeloid blood toxicity screens identifies compounds promoting and inhibiting endothelial-to-hematopoietic transition

Mon, 01 Jan 0001 00:00:00 +0000

A novel hiPSC-derived system for hematoendothelial and myeloid blood toxicity screens identifies compounds promoting and inhibiting endothelial-to-hematopoietic transition

DOI: 10.1016/j.tiv.2019.104622

Authors: Irina Elcheva; Mechelle Sneed; Scott Frazee; Zhenqiu Liu; Junjia Zhu; Tyler Wood; Sara Hendrickson; Chuck Oehler; Brad Garcia; Vladimir S. Spiegelman

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “Matrigel” at “N/A” “N/A”.

Ideally, “PSC” should be obtained at the end of culture.

A Perfused In Vitro Human iPSC-Derived Blood–Brain Barrier Faithfully Mimics Transferrin Receptor-Mediated Transcytosis of Therapeutic Antibodies

Mon, 01 Jan 0001 00:00:00 +0000

A Perfused In Vitro Human iPSC-Derived Blood–Brain Barrier Faithfully Mimics Transferrin Receptor-Mediated Transcytosis of Therapeutic Antibodies

DOI: 10.1007/s10571-023-01404-x

Authors: Floriana Burgio; Carine Gaiser; Kevin Brady; Viviana Gatta; Reiner Class; Ramona Schrage; Laura Suter-Dick

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “Matrigel” at “0.333” “g/l”.

Ideally, “PSC” should express “POU5F1”, “SSEA4”, “TRA-1-60”, “SOX2”, “ZO1”, “Occludin”, “transferrin receptor”, “P-gp” at the end of culture.

A prenatal skin atlas reveals immune regulation of human skin morphogenesis

Mon, 01 Jan 0001 00:00:00 +0000

A prenatal skin atlas reveals immune regulation of human skin morphogenesis

DOI: 10.1038/s41586-024-08002-x

Authors: Nusayhah Hudaa Gopee; Elena Winheim; Bayanne Olabi; Chloe Admane; April Rose Foster; Ni Huang; Rachel A. Botting; Fereshteh Torabi; Dinithi Sumanaweera; Anh Phuong Le; Jin Kim; Luca Verger; Emily Stephenson; Diana Adão; Clarisse Ganier; Kelly Y. Gim; Sara A. Serdy; CiCi Deakin; Issac Goh; Lloyd Steele; Karl Annusver; Mohi-Uddin Miah; Win Min Tun; Pejvak Moghimi; Kwasi Amoako Kwakwa; Tong Li; Daniela Basurto Lozada; Ben Rumney; Catherine L. Tudor; Kenny Roberts; Nana-Jane Chipampe; Keval Sidhpura; Justin Englebert; Laura Jardine; Gary Reynolds; Antony Rose; Vicky Rowe; Sophie Pritchard; Ilaria Mulas; James Fletcher; Dorin-Mirel Popescu; Elizabeth Poyner; Anna Dubois; Alyson Guy; Andrew Filby; Steven Lisgo; Roger A. Barker; Ian A. Glass; Jong-Eun Park; Roser Vento-Tormo; Marina Tsvetomilova Nikolova; Peng He; John E. G. Lawrence; Josh Moore; Stephane Ballereau; Christine B. Hale; Vijaya Shanmugiah; David Horsfall; Neil Rajan; John A. McGrath; Edel A. O’Toole; Barbara Treutlein; Omer Bayraktar; Maria Kasper; Fränze Progatzky; Pavel Mazin; Jiyoon Lee; Laure Gambardella; Karl R. Koehler; Sarah A. Teichmann; Muzlifah Haniffa

A protocol for rapid pericyte differentiation of human induced pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

A protocol for rapid pericyte differentiation of human induced pluripotent stem cells

DOI: 10.1016/j.xpro.2020.100261

Authors: Elizabeth A. Aisenbrey; Elizabeth Torr; Hunter Johnson; Cheryl Soref; William Daly; William L. Murphy

Step 1: iPSC/ESC maintainance

day 0 ~ 2

culture “adherent” human PSC in “E8” for “2” days. For ECM, use “Matrigel” at “0.06” “g/m2”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

	concentration	unit
Y-27623	1.0e-5	mol/l

Ideally, “PSC” should be obtained at the end of culture.

A Role for the Long Noncoding RNA SENCR in Commitment and Function of Endothelial Cells

Mon, 01 Jan 0001 00:00:00 +0000

A Role for the Long Noncoding RNA SENCR in Commitment and Function of Endothelial Cells

DOI: 10.1038/mt.2016.41

Authors: Mounia Boulberdaa; Elizabeth Scott; Margaret Ballantyne; Raquel Garcia; Betty Descamps; Gianni D Angelini; Mairi Brittan; Amanda Hunter; Martin McBride; John McClure; Joseph M Miano; Costanza Emanueli; Nicholas L Mills; Joanne C Mountford; Andrew H Baker

Step 1: mesoderm 1

day 0 ~ 3

culture “spheroid” human ESC in “unspecified medium” for “3” days. Medium should be changed once in “3” days.

A Scalable and Efficient Bioprocess for Manufacturing Human Pluripotent Stem Cell-Derived Endothelial Cells

Mon, 01 Jan 0001 00:00:00 +0000

A Scalable and Efficient Bioprocess for Manufacturing Human Pluripotent Stem Cell-Derived Endothelial Cells

DOI: 10.1016/j.stemcr.2018.07.001

Authors: Haishuang Lin; Qian Du; Qiang Li; Ou Wang; Zhanqi Wang; Neety Sahu; Christian Elowsky; Kan Liu; Chi Zhang; Soonkyu Chung; Bin Duan; Yuguo Lei

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “spheroid” human PSC in “E8” for “1” days. For ECM, use “PNIPAAm-PEG hydrogel” at “10” “%”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

A Simplified, Fully Defined Differentiation Scheme for Producing Blood-Brain Barrier Endothelial Cells from Human iPSCs

Mon, 01 Jan 0001 00:00:00 +0000

A Simplified, Fully Defined Differentiation Scheme for Producing Blood-Brain Barrier Endothelial Cells from Human iPSCs

DOI: 10.1016/j.stemcr.2019.05.008

Authors: Emma H. Neal; Nicholas A. Marinelli; Yajuan Shi; P. Mason McClatchey; Kylie M. Balotin; Dalton R. Gullett; Kameron A. Hagerla; Aaron B. Bowman; Kevin C. Ess; John P. Wikswo; Ethan S. Lippmann

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “E8” for “1” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

A single-cell level comparison of human inner ear organoids with the human cochlea and vestibular organs

Mon, 01 Jan 0001 00:00:00 +0000

A single-cell level comparison of human inner ear organoids with the human cochlea and vestibular organs

DOI: 10.1016/j.celrep.2023.112623

Authors: Wouter H. van der Valk; Edward S.A. van Beelen; Matthew R. Steinhart; Carl Nist-Lund; Daniel Osorio; John C.M.J. de Groot; Liang Sun; Peter Paul G. van Benthem; Karl R. Koehler; Heiko Locher

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “E8” for “1” days. For ECM, use “vitronectin” at “5.0e-7” “g/m”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

A synthetic substrate to support early mesodermal differentiation of human embryonic stem cells

Mon, 01 Jan 0001 00:00:00 +0000

A synthetic substrate to support early mesodermal differentiation of human embryonic stem cells

DOI: 10.1016/j.biomaterials.2011.07.052

Authors: Yang Liu; Xintong Wang; Dan S. Kaufman; Wei Shen

Step 1: mesoderm 1

day 0 ~ 2

culture “adherent” unknown species cell and human ESC in “unspecified medium” for “2” days. For ECM, use “substrate functionalized with Lα5β1 and Lα6β1” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

A Tissue Engineered Blood Vessel Model of Hutchinson-Gilford Progeria Syndrome Using Human iPSC-derived Smooth Muscle Cells

Mon, 01 Jan 0001 00:00:00 +0000

A Tissue Engineered Blood Vessel Model of Hutchinson-Gilford Progeria Syndrome Using Human iPSC-derived Smooth Muscle Cells

DOI: 10.1038/s41598-017-08632-4

Authors: Leigh Atchison; Haoyue Zhang; Kan Cao; George A. Truskey

No steps available

Accelerated differentiation of human induced pluripotent stem cells to blood–brain barrier endothelial cells

Mon, 01 Jan 0001 00:00:00 +0000

Accelerated differentiation of human induced pluripotent stem cells to blood–brain barrier endothelial cells

DOI: 10.1186/s12987-017-0059-0

Authors: Emma K. Hollmann; Amanda K. Bailey; Archit V. Potharazu; M. Diana Neely; Aaron B. Bowman; Ethan S. Lippmann

Step 1: iPSC/ESC maintainance

day 0 ~ 1

Activation of lineage competence in hemogenic endothelium precedes the formation of hematopoietic stem cell heterogeneity

Mon, 01 Jan 0001 00:00:00 +0000

Activation of lineage competence in hemogenic endothelium precedes the formation of hematopoietic stem cell heterogeneity

DOI: 10.1038/s41422-023-00797-0

Authors: Jun Xia; Mengyao Liu; Caiying Zhu; Shicheng Liu; Lanlan Ai; Dongyuan Ma; Ping Zhu; Lu Wang; Feng Liu

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “Matrigel” at “N/A” “N/A”.

Step 2: Primitive streak 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

Adaptation of Human Testicular Niche Cells for Pluripotent Stem Cell and Testis Development Research

Mon, 01 Jan 0001 00:00:00 +0000

Adaptation of Human Testicular Niche Cells for Pluripotent Stem Cell and Testis Development Research

DOI: 10.1007/s13770-020-00240-0

Authors: Marina V. Pryzhkova; Philip W. Jordan

Step 1: iPSC/ESC maintainance

day 0 ~ 5

culture “adherent” human ESC in “E8” for “5” days. For ECM, use “Geltrex” at “N/A” “N/A”. Medium should be changed once in “5” days.

Step 2: Primitive streak 1

day 5 ~ 6

culture “spheroid” human ESC in “E8” for “1” days. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Aligned nanofiber scaffolds improve functionality of cardiomyocytes differentiated from human induced pluripotent stem cell-derived cardiac progenitor cells

Mon, 01 Jan 0001 00:00:00 +0000

Aligned nanofiber scaffolds improve functionality of cardiomyocytes differentiated from human induced pluripotent stem cell-derived cardiac progenitor cells

DOI: 10.1038/s41598-020-70547-4

Authors: Mei Ding; Henrik Andersson; Sofia Martinsson; Alan Sabirsh; Anna Jonebring; Qing-Dong Wang; Alleyn T. Plowright; Lauren Drowley

No steps available

An unexpected role of IL10 in mesoderm induction and differentiation from pluripotent stem cells: Implications in zebrafish angiogenic sprouting, vascular organoid development, and therapeutic angiogenesis

Mon, 01 Jan 0001 00:00:00 +0000

An unexpected role of IL10 in mesoderm induction and differentiation from pluripotent stem cells: Implications in zebrafish angiogenic sprouting, vascular organoid development, and therapeutic angiogenesis

DOI: 10.1016/j.ejcb.2024.151465

Authors: Kaiyuan Niu; Chengxin Zhang; Chenxin Liu; Wei Wu; Yi Yan; Ancheng Zheng; Silin Liu; Zhenning Shi; Mei Yang; Wen Wang; Qingzhong Xiao

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “Matrigel” at “10” “%”. Medium should contain factors shown in the following table.

Analysis of endothelial-to-haematopoietic transition at the single cell level identifies cell cycle regulation as a driver of differentiation

Mon, 01 Jan 0001 00:00:00 +0000

Analysis of endothelial-to-haematopoietic transition at the single cell level identifies cell cycle regulation as a driver of differentiation

DOI: 10.1186/s13059-020-02058-4

Authors: Giovanni Canu; Emmanouil Athanasiadis; Rodrigo A. Grandy; Jose Garcia-Bernardo; Paulina M. Strzelecka; Ludovic Vallier; Daniel Ortmann; Ana Cvejic

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “E6 medium”. For ECM, use “Vitronectin” at “0.01” “g/l”. Medium should contain factors shown in the following table.

Angiogenic activity mediates bone repair from human pluripotent stem cell-derived osteogenic cells

Mon, 01 Jan 0001 00:00:00 +0000

Angiogenic activity mediates bone repair from human pluripotent stem cell-derived osteogenic cells

DOI: 10.1038/srep22868

Authors: Li Zou; Qingshan Chen; Zachary Quanbeck; Joan E. Bechtold; Dan S. Kaufman

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “unspecified medium” for “1” days. Medium should be changed once in “1” days.

Ideally, “PSC” should express “NANOG”, “SOX2”, “POU5F1”, “SSEA4”, “Tra-1-60”, “Tra-1-81” at the end of culture.

Arterial specification of endothelial cells derived from human induced pluripotent stem cells in a biomimetic flow bioreactor

Mon, 01 Jan 0001 00:00:00 +0000

Arterial specification of endothelial cells derived from human induced pluripotent stem cells in a biomimetic flow bioreactor

DOI: 10.1016/j.biomaterials.2015.02.121

Authors: Amogh Sivarapatna; Mahboobe Ghaedi; Andrew V. Le; Julio J. Mendez; Yibing Qyang; Laura E. Niklason

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “mTeSR” for “1” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Assembly and Function of a Bioengineered Human Liver for Transplantation Generated Solely from Induced Pluripotent Stem Cells

Mon, 01 Jan 0001 00:00:00 +0000

Assembly and Function of a Bioengineered Human Liver for Transplantation Generated Solely from Induced Pluripotent Stem Cells

DOI: 10.1016/j.celrep.2020.107711

Authors: Kazuki Takeishi; Alexandra Collin de l’Hortet; Yang Wang; Kan Handa; Jorge Guzman-Lepe; Kentaro Matsubara; Kazutoyo Morita; Sae Jang; Nils Haep; Rodrigo M. Florentino; Fangchao Yuan; Ken Fukumitsu; Kimimasa Tobita; Wendell Sun; Jonathan Franks; Evan R. Delgado; Erik M. Shapiro; Nicolas A. Fraunhoffer; Andrew W. Duncan; Hiroshi Yagi; Tomoji Mashimo; Ira J. Fox; Alejandro Soto-Gutierrez

Assembly of Human Stem Cell-Derived Cortical Spheroids and Vascular Spheroids to Model 3-D Brain-like Tissues

Mon, 01 Jan 0001 00:00:00 +0000

Assembly of Human Stem Cell-Derived Cortical Spheroids and Vascular Spheroids to Model 3-D Brain-like Tissues

DOI: 10.1038/s41598-019-42439-9

Authors: Liqing Song; Xuegang Yuan; Zachary Jones; Kyle Griffin; Yi Zhou; Teng Ma; Yan Li

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “Geltrex” at “N/A” “N/A”. Medium should contain factors shown in the following table.

	concentration	unit
Y27632	1.0e-5	mol/l

Step 2: Primitive streak 1

day 1 ~ 4

Assessing and enhancing migration of human myogenic progenitors using directed iPS cell differentiation and advanced tissue modelling

Mon, 01 Jan 0001 00:00:00 +0000

Assessing and enhancing migration of human myogenic progenitors using directed

 <scp>iPS</scp>
cell differentiation and advanced tissue modelling

DOI: 10.15252/emmm.202114526

Authors: SungWoo Choi; Giulia Ferrari; Louise A Moyle; Kirsty Mackinlay; Naira Naouar; Salma Jalal; Sara Benedetti; Christine Wells; Francesco Muntoni; Francesco Saverio Tedesco

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “E8”. For ECM, use “vitronectin” at “N/A” “N/A”.

Assessment of iPSC teratogenicity throughout directed differentiation toward an alveolar-like phenotype

Mon, 01 Jan 0001 00:00:00 +0000

Assessment of iPSC teratogenicity throughout directed differentiation toward an alveolar-like phenotype

DOI: 10.1016/j.diff.2019.01.003

Authors: Adam Mitchell; Heather Wanczyk; Todd Jensen; Christine Finck

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “StemMACS iPS-Brew XF”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table.

	concentration	unit
Y27632	1.0e-5	mol/l

Ideally, “PSC” should be obtained at the end of culture.

Autologous endothelialized small-caliber vascular grafts engineered from blood-derived induced pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

Autologous endothelialized small-caliber vascular grafts engineered from blood-derived induced pluripotent stem cells

DOI: 10.1016/j.actbio.2019.07.032

Authors: Melanie Generali; Elisa A. Casanova; Debora Kehl; Debora Wanner; Simon P. Hoerstrup; Paolo Cinelli; Benedikt Weber

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “DMEM/F12”. Medium should contain factors shown in the following table.

	concentration	unit
KSR	20	%
NEAA	0.1	mol/l
2-mercaptoethanol	0.05	mol/l
Glutamine	0.001	mol/l
PSA	50000	U/l
FGF	1.0e-5	g/l

Ideally, “PSC” should express “POU5F1”, “SOX2”, “NANOG”, “REX1”, “DPPA3”, “TRA-1-81”, “TRA-1-60” at the end of culture.

Automated Deep Learning-Based System to Identify Endothelial Cells Derived from Induced Pluripotent Stem Cells

Mon, 01 Jan 0001 00:00:00 +0000

Automated Deep Learning-Based System to Identify Endothelial Cells Derived from Induced Pluripotent Stem Cells

DOI: 10.1016/j.stemcr.2018.04.007

Authors: Dai Kusumoto; Mark Lachmann; Takeshi Kunihiro; Shinsuke Yuasa; Yoshikazu Kishino; Mai Kimura; Toshiomi Katsuki; Shogo Itoh; Tomohisa Seki; Keiichi Fukuda

Step 1: iPSC/ESC maintainance

day 0 ~ 2

culture “adherent” unknown species PSC in “mTeSR” for “2” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

BCL6B-dependent suppression of ETV2 hampers endothelial cell differentiation

Mon, 01 Jan 0001 00:00:00 +0000

BCL6B-dependent suppression of ETV2 hampers endothelial cell differentiation

DOI: 10.1186/s13287-024-03832-y

Authors: Zhonghao Li; Wei Wu; Qiushi Li; Xin Heng; Wei Zhang; Yinghong Zhu; Lin Chen; Ziqi Chen; Mengcheng Shen; Ning Ma; Qingzhong Xiao; Yi Yan

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “E8” for “1” days. “PSC” should be “upregulated” for “BCL6B”, “downregulated” for “BCL6B”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Bioengineering of a human iPSC-derived vascularized endocrine pancreas for type 1 diabetes

Mon, 01 Jan 0001 00:00:00 +0000

Bioengineering of a human iPSC-derived vascularized endocrine pancreas for type 1 diabetes

DOI: 10.1016/j.xcrm.2025.101938

Authors: Francesco Campo; Alessia Neroni; Cataldo Pignatelli; Silvia Pellegrini; Ilaria Marzinotto; Libera Valla; Fabio Manenti; Martina Policardi; Vito Lampasona; Lorenzo Piemonti; Antonio Citro

No steps available

Bioorthogonal non-canonical amino acid tagging to track transplanted human induced pluripotent stem cell-specific proteome

Mon, 01 Jan 0001 00:00:00 +0000

Bioorthogonal non-canonical amino acid tagging to track transplanted human induced pluripotent stem cell-specific proteome

DOI: 10.1186/s13287-024-03792-3

Authors: Divya Sridharan; Julie A. Dougherty; Uzair Ahmed; Shridhar K. Sanghvi; Syed Baseeruddin Alvi; Ki Ho Park; Helena Islam; Sue E. Knoblaugh; Harpreet Singh; Elizabeth D. Kirby; Mahmood Khan

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “E8” for “1” days. “PSC” should be “upregulated” for “L274G”. For ECM, use “Cell Basement Membrane” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Bioprinting of human pluripotent stem cell derived corneal endothelial cells with hydrazone crosslinked hyaluronic acid bioink

Mon, 01 Jan 0001 00:00:00 +0000

Bioprinting of human pluripotent stem cell derived corneal endothelial cells with hydrazone crosslinked hyaluronic acid bioink

DOI: 10.1186/s13287-024-03672-w

Authors: Pyry Grönroos; Anni Mörö; Paula Puistola; Karoliina Hopia; Maija Huuskonen; Taina Viheriälä; Tanja Ilmarinen; Heli Skottman

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “E8” for “1” days. For ECM, use “Laminin-521” at “N/A” “N/A”. Medium should be changed once in “1” days.

Ideally, “PSC” should be obtained at the end of culture.

Biotransformation of nanoplastics in human plasma and their permeation through a model in vitro blood-brain barrier: An in-depth quantitative analysis

Mon, 01 Jan 0001 00:00:00 +0000

Biotransformation of nanoplastics in human plasma and their permeation through a model in vitro blood-brain barrier: An in-depth quantitative analysis

DOI: 10.1016/j.nantod.2024.102466

Authors: Fazel Abdolahpur Monikh; Šárka Lehtonen; Jukka Kekäläinen; Isabel Karkossa; Seppo Auriola; Kristin Schubert; Alessandra Zanut; Sanni Peltonen; Jonna Niskanen; Mandar Bandekar; Martin von Bergen; Jari T.T. Leskinen; Arto Koistinen; Sara Bogialli; Zhiling Guo; Jussi V.K. Kukkonen; Chunying Chen; Iseult Lynch

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

Biphasic modulation of insulin signaling enables highly efficient hematopoietic differentiation from human pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

Biphasic modulation of insulin signaling enables highly efficient hematopoietic differentiation from human pluripotent stem cells

DOI: 10.1186/s13287-018-0934-x

Authors: Fuyu Duan; Rujin Huang; Fengzhi Zhang; Yonglin Zhu; Lin Wang; Xia Chen; Lufeng Bai; Wei Guo; Sophia Chia-Ning Chang; Xiaoyu Hu; Jie Na

Step 1: iPSC/ESC maintainance

day 0 ~ 2

Blocking autocrine VEGF signaling by sunitinib, an anti-cancer drug, promotes embryonic stem cell self-renewal and somatic cell reprogramming

Mon, 01 Jan 0001 00:00:00 +0000

Blocking autocrine VEGF signaling by sunitinib, an anti-cancer drug, promotes embryonic stem cell self-renewal and somatic cell reprogramming

DOI: 10.1038/cr.2014.112

Authors: Guofang Chen; Xinxiu Xu; Lihong Zhang; Yanbin Fu; Min Wang; Haifeng Gu; Xin Xie

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” mouse PSC in “mES medium” for “1” days. For ECM, use “Gelatin” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Blood-generating heart-forming organoids recapitulate co-development of the human haematopoietic system and the embryonic heart

Mon, 01 Jan 0001 00:00:00 +0000

Blood-generating heart-forming organoids recapitulate co-development of the human haematopoietic system and the embryonic heart

DOI: 10.1038/s41556-024-01526-4

Authors: Miriana Dardano; Felix Kleemiß; Maike Kosanke; Dorina Lang; Liam Wilson; Annika Franke; Jana Teske; Akshatha Shivaraj; Jeanne de la Roche; Martin Fischer; Lucas Lange; Axel Schambach; Lika Drakhlis; Robert Zweigerdt

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “E8” for “1” days. For ECM, use “Geltrex” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Bone marrow-derived pluripotent very small embryonic-like stem cells (VSELs) are mobilized after acute myocardial infarction

Mon, 01 Jan 0001 00:00:00 +0000

Bone marrow-derived pluripotent very small embryonic-like stem cells (VSELs) are mobilized after acute myocardial infarction

DOI: 10.1016/j.yjmcc.2008.02.279

Authors: Ewa K. Zuba-Surma; Magdalena Kucia; Buddhadeb Dawn; Yiru Guo; Mariusz Z. Ratajczak; Roberto Bolli

No steps available

Brain infiltration of breast cancer stem cells is facilitated by paracrine signaling by inhibitor of differentiation 3 to nuclear respiratory factor 1

Mon, 01 Jan 0001 00:00:00 +0000

Brain infiltration of breast cancer stem cells is facilitated by paracrine signaling by inhibitor of differentiation 3 to nuclear respiratory factor 1

DOI: 10.1007/s00432-022-04026-w

Authors: Jayanta K. Das; Alok Deoraj; Deodutta Roy; Quentin Felty

No steps available

Brain microvascular endothelial cell dysfunction in an isogenic juvenile iPSC model of Huntington’s disease

Mon, 01 Jan 0001 00:00:00 +0000

Brain microvascular endothelial cell dysfunction in an isogenic juvenile iPSC model of Huntington’s disease

DOI: 10.1186/s12987-022-00347-7

Authors: Raleigh M. Linville; Renée F. Nerenberg; Gabrielle Grifno; Diego Arevalo; Zhaobin Guo; Peter C. Searson

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “mTeSR” for “1” days. For ECM, use “Matrigel” at “0.083” “g/l”. Medium should be changed once in “1” days.

Ideally, “PSC” should be obtained at the end of culture.

Breast cancer-on-chip for patient-specific efficacy and safety testing of CAR-T cells

Mon, 01 Jan 0001 00:00:00 +0000

Breast cancer-on-chip for patient-specific efficacy and safety testing of CAR-T cells

DOI: 10.1016/j.stem.2024.04.018

Authors: Tengku Ibrahim Maulana; Claudia Teufel; Madalena Cipriano; Julia Roosz; Lisa Lazarevski; Francijna E. van den Hil; Lukas Scheller; Valeria Orlova; André Koch; Michael Hudecek; Miriam Alb; Peter Loskill

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “E8”. For ECM, use “Vitronectin” at “N/A” “N/A”.

Ideally, “PSC” should express “OCT3/4”, “SSEA-4”, “NANOG” at the end of culture.

cAMP/EPAC Signaling Enables ETV2 to Induce Endothelial Cells with High Angiogenesis Potential

Mon, 01 Jan 0001 00:00:00 +0000

cAMP/EPAC Signaling Enables ETV2 to Induce Endothelial Cells with High Angiogenesis Potential

DOI: 10.1016/j.ymthe.2019.11.019

Authors: Jae-Jun Kim; Da-Hyun Kim; Jin Young Lee; Byung-Chul Lee; Insung Kang; Myung Geun Kook; Dasom Kong; Soon Won Choi; Heung-Myong Woo; Dong-Ik Kim; Kyung-Sun Kang

Step 1: endothelial 1

day 0 ~ 2

culture “adherent” human Fibroblast in “EGM2” for “2” days. “Fibroblast” should be “upregulated” for “ETV2”. For ECM, use “Collagen” at “0.02” “g/l”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

CD13 and ROR2 Permit Isolation of Highly Enriched Cardiac Mesoderm from Differentiating Human Embryonic Stem Cells

Mon, 01 Jan 0001 00:00:00 +0000

CD13 and ROR2 Permit Isolation of Highly Enriched Cardiac Mesoderm from Differentiating Human Embryonic Stem Cells

DOI: 10.1016/j.stemcr.2015.11.006

Authors: Rhys J.P. Skelton; Bevin Brady; Suhail Khoja; Debashis Sahoo; James Engel; Deevina Arasaratnam; Kholoud K. Saleh; Oscar J. Abilez; Peng Zhao; Edouard G. Stanley; Andrew G. Elefanty; Murray Kwon; David A. Elliott; Reza Ardehali

Step 1: mesoderm 1

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” unknown species cell in “unspecified medium”.

CD32 captures committed haemogenic endothelial cells during human embryonic development

Mon, 01 Jan 0001 00:00:00 +0000

CD32 captures committed haemogenic endothelial cells during human embryonic development

DOI: 10.1038/s41556-024-01403-0

Authors: Rebecca Scarfò; Lauren N. Randolph; Monah Abou Alezz; Mahassen El Khoury; Amélie Gersch; Zhong-Yin Li; Stephanie A. Luff; Andrea Tavosanis; Giulia Ferrari Ramondo; Sara Valsoni; Sara Cascione; Emma Didelon; Laura Passerini; Giada Amodio; Chiara Brandas; Anna Villa; Silvia Gregori; Ivan Merelli; Jean-Noël Freund; Christopher M. Sturgeon; Manuela Tavian; Andrea Ditadi

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

Cell-type-specific regulation of APOE and CLU levels in human neurons by the Alzheimer’s disease risk gene SORL1

Mon, 01 Jan 0001 00:00:00 +0000

Cell-type-specific regulation of APOE and CLU levels in human neurons by the Alzheimer’s disease risk gene SORL1

DOI: 10.1016/j.celrep.2023.112994

Authors: Hyo Lee; Aimee J. Aylward; Richard V. Pearse; Alexandra M. Lish; Yi-Chen Hsieh; Zachary M. Augur; Courtney R. Benoit; Vicky Chou; Allison Knupp; Cheryl Pan; Srilakshmi Goberdhan; Duc M. Duong; Nicholas T. Seyfried; David A. Bennett; Mariko F. Taga; Kevin Huynh; Matthias Arnold; Peter J. Meikle; Philip L. De Jager; Vilas Menon; Jessica E. Young; Tracy L. Young-Pearse

Cells sorted off hiPSC-derived kidney organoids coupled with immortalized cells reliably model the proximal tubule

Mon, 01 Jan 0001 00:00:00 +0000

Cells sorted off hiPSC-derived kidney organoids coupled with immortalized cells reliably model the proximal tubule

DOI: 10.1038/s42003-023-04862-7

Authors: Ramin Banan Sadeghian; Ryohei Ueno; Yuji Takata; Akihiko Kawakami; Cheng Ma; Toshikazu Araoka; Minoru Takasato; Ryuji Yokokawa

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “StemFit”. For ECM, use “Laminin” at “0.003” “g/l”. Medium should contain factors shown in the following table.

Changes of plasmalogen phospholipid levels during differentiation of induced pluripotent stem cells 409B2 to endothelial phenotype cells

Mon, 01 Jan 0001 00:00:00 +0000

Changes of plasmalogen phospholipid levels during differentiation of induced pluripotent stem cells 409B2 to endothelial phenotype cells

DOI: 10.1038/s41598-017-09980-x

Authors: Yusuke Nakamura; Yasuo Shimizu; Yasuhiro Horibata; Rinna Tei; Ryosuke Koike; Meitetsu Masawa; Taiji Watanabe; Taichi Shiobara; Ryo Arai; Kazuyuki Chibana; Akihiro Takemasa; Hiroyuki Sugimoto; Yoshiki Ishii

Step 1: iPSC/ESC maintainance

day 0 ~ 2

culture “adherent” human PSC in “StemFit medium” for “2” days. For ECM, use “laminin-511 E8” at “N/A” “N/A”. Medium should be changed once in “2” days.

Characterization of in vivo chemoresistant human hepatocellular carcinoma cells with transendothelial differentiation capacities

Mon, 01 Jan 0001 00:00:00 +0000

Characterization of in vivo chemoresistant human hepatocellular carcinoma cells with transendothelial differentiation capacities

DOI: 10.1186/1471-2407-13-176

Authors: Christian Marfels; Miriam Hoehn; Ernst Wagner; Michael Günther

Step 1: endothelial 1

day 0 ~ 42

culture “unspecified method” unknown species cell in “unspecified medium” for “42” days. For ECM, use “agarose” at “N/A” “N/A”. Medium should be changed once in “42” days.

Step 2: endothelial 2

day 42 ~ 43

culture “unspecified method” unknown species cell in “unspecified medium” for “1” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should be changed once in “1” days.

Characterization of the proteome and metabolome of human liver sinusoidal endothelial-like cells derived from induced pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

Characterization of the proteome and metabolome of human liver sinusoidal endothelial-like cells derived from induced pluripotent stem cells

DOI: 10.1016/j.diff.2021.06.001

Authors: Mathieu Danoy; Rachid Jellali; Yannick Tauran; Johanna Bruce; Marjorie Leduc; Françoise Gilard; Bertrand Gakière; Benedikt Scheidecker; Taketomo Kido; Atsushi Miyajima; Fabrice Soncin; Yasuyuki Sakai; Eric Leclerc

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” human PSC in “unspecified medium”.

Step 2: endothelial 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

Chemically defined human vascular laminins for biologically relevant culture of hiPSC-derived brain microvascular endothelial cells

Mon, 01 Jan 0001 00:00:00 +0000

Chemically defined human vascular laminins for biologically relevant culture of hiPSC-derived brain microvascular endothelial cells

DOI: 10.1186/s12987-020-00215-2

Authors: Pedram Motallebnejad; Samira M. Azarin

Step 1: endothelial 1

day 0 ~ 1

culture “adherent” human PSC in “human endothelial serum-free medium” for “1” days. For ECM, use “laminin 511-E8” at “0.01” “g/m2”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

	concentration	unit
bovine platelet-poor plasma derived serum	1	%
FBS	2	%
FGF	2.0e-5	g/l
retinoic acid	1.0e-5	mol/l

Ideally, “EC” should be obtained at the end of culture.

Chemically-defined albumin-free differentiation of human pluripotent stem cells to endothelial progenitor cells

Mon, 01 Jan 0001 00:00:00 +0000

Chemically-defined albumin-free differentiation of human pluripotent stem cells to endothelial progenitor cells

DOI: 10.1016/j.scr.2015.05.004

Authors: Xiaoping Bao; Xiaojun Lian; Kaitlin K. Dunn; Mengxuan Shi; Tianxiao Han; Tongcheng Qian; Vijesh J. Bhute; Scott G. Canfield; Sean P. Palecek

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “E8” for “1” days. For ECM, use “Synthemax” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Chronic nicotine impairs the angiogenic capacity of human induced pluripotent stem cell-derived endothelial cells in a murine model of peripheral arterial disease

Mon, 01 Jan 0001 00:00:00 +0000

Chronic nicotine impairs the angiogenic capacity of human induced pluripotent stem cell-derived endothelial cells in a murine model of peripheral arterial disease

DOI: 10.1016/j.jvssci.2023.100115

Authors: Alex H.P. Chan; Caroline Hu; Gladys C.F. Chiang; Chisomaga Ekweume; Ngan F. Huang

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” human PSC in “E8”.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: mesoderm 1

day 1 ~ 5

Closing the Mitochondrial Permeability Transition Pore in hiPSC-Derived Endothelial Cells Induces Glycocalyx Formation and Functional Maturation

Mon, 01 Jan 0001 00:00:00 +0000

Closing the Mitochondrial Permeability Transition Pore in hiPSC-Derived Endothelial Cells Induces Glycocalyx Formation and Functional Maturation

DOI: 10.1016/j.stemcr.2019.10.005

Authors: Gesa L. Tiemeier; Gangqi Wang; Sébastien J. Dumas; Wendy M.P.J. Sol; M. Cristina Avramut; Tobias Karakach; Valeria V. Orlova; Cathelijne W. van den Berg; Christine L. Mummery; Peter Carmeliet; Bernard M. van den Berg; Ton J. Rabelink

Step 1: iPSC/ESC maintainance

day 0 ~ 4

culture “adherent” human PSC in “E8” for “4” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should be changed once in “4” days.

Co-culture of human induced pluripotent stem cell-derived retinal pigment epithelial cells and endothelial cells on double collagen-coated honeycomb films

Mon, 01 Jan 0001 00:00:00 +0000

Co-culture of human induced pluripotent stem cell-derived retinal pigment epithelial cells and endothelial cells on double collagen-coated honeycomb films

DOI: 10.1016/j.actbio.2019.11.002

Authors: Maria Teresa Calejo; Jaakko Saari; Hanna Vuorenpää; Elina Vuorimaa-Laukkanen; Pasi Kallio; Katriina Aalto-Setälä; Susanna Miettinen; Heli Skottman; Minna Kellomäki; Kati Juuti-Uusitalo

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” human PSC in “E8”.

Ideally, “PSC” should be obtained at the end of culture.

Combinational regenerative inductive effect of bio-adhesive hybrid hydrogels conjugated with hiPSC-derived myofibers and its derived EVs for volumetric muscle regeneration

Mon, 01 Jan 0001 00:00:00 +0000

Combinational regenerative inductive effect of bio-adhesive hybrid hydrogels conjugated with hiPSC-derived myofibers and its derived EVs for volumetric muscle regeneration

DOI: 10.1016/j.bioactmat.2024.09.013

Authors: Jiseong Kim; Myung Chul Lee; Jieun Jeon; Alejandra Rodríguez-delaRosa; Yori Endo; Da-Seul Kim; Andrea Donaxi Madrigal-Salazar; Jeong Wook Seo; Hyeseon Lee; Ki-Tae Kim; Jae-I Moon; Seung Gwa Park; Mariana Carolina Lopez-Pacheco; Abdulhameed F. Alkhateeb; Nebras Sobahi; Nicole Bassous; Wenpeng Liu; Jae Seo Lee; Seongsoo Kim; Dilara Yilmaz Aykut; Mahmoud Lotfi Nasr; Mohammad Asif Hussain; Soo-Hong Lee; Woo-Jin Kim; Olivier Pourquié; Indranil Sinha; Su Ryon Shin

Combinatorial Extracellular Matrix Microenvironments for Probing Endothelial Differentiation of Human Pluripotent Stem Cells

Mon, 01 Jan 0001 00:00:00 +0000

Combinatorial Extracellular Matrix Microenvironments for Probing Endothelial Differentiation of Human Pluripotent Stem Cells

DOI: 10.1038/s41598-017-06986-3

Authors: Luqia Hou; Joseph J. Kim; Maureen Wanjare; Bhagat Patlolla; John Coller; Vanita Natu; Trevor J. Hastie; Ngan F. Huang

Step 1: endothelial 1

day 0 ~ 2

culture “device” human PSC in “alpha-MEM” for “2” days. For ECM, use “collagen IV+heparan sulfate+laminin” at “0.5” “g/l”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Combinatorial extracellular matrix microenvironments promote survival and phenotype of human induced pluripotent stem cell-derived endothelial cells in hypoxia

Mon, 01 Jan 0001 00:00:00 +0000

Combinatorial extracellular matrix microenvironments promote survival and phenotype of human induced pluripotent stem cell-derived endothelial cells in hypoxia

DOI: 10.1016/j.actbio.2016.08.003

Authors: Luqia Hou; John Coller; Vanita Natu; Trevor J. Hastie; Ngan F. Huang

Step 1: endothelial 1

day 0 ~ 14

culture “unspecified method” human PSC in “unspecified medium” for “14” days. Medium should contain factors shown in the following table, and should be changed once in “14” days.

	concentration	unit
VEGF	—	—
BMP4	—	—

Ideally, “EC” should express “PECAM1”, “VWF” at the end of culture.

Commentary on human pluripotent stem cell-based blood–brain barrier models

Mon, 01 Jan 0001 00:00:00 +0000

Commentary on human pluripotent stem cell-based blood–brain barrier models

DOI: 10.1186/s12987-020-00222-3

Authors: Ethan S. Lippmann; Samira M. Azarin; Sean P. Palecek; Eric V. Shusta

Step 1: endothelial 1

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” unknown species cell and human PSC in “unspecified medium”. Medium should contain factors shown in the following table.

	concentration	unit
retinoic acid	—	—

Ideally, “EC” should express “PECAM1”, “CDH5”, “VWF”, “KDR”, “TEK”, “SOX17” at the end of culture.

Complement anaphylatoxins C3a and C5a induce a failing regenerative program in cardiac resident cells. Evidence of a role for cardiac resident stem cells other than cardiomyocyte renewal

Mon, 01 Jan 0001 00:00:00 +0000

Complement anaphylatoxins C3a and C5a induce a failing regenerative program in cardiac resident cells. Evidence of a role for cardiac resident stem cells other than cardiomyocyte renewal

DOI: 10.1186/2193-1801-1-63

Authors: David Lara-Astiaso; Alberto Izarra; Juan Camilo Estrada; Carmen Albo; Isabel Moscoso; Enrique Samper; Javier Moncayo; Abelardo Solano; Antonio Bernad; Antonio Díez-Juan

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” mouse CPC in “DMEM/F12”. For ECM, use “Gelatin” at “0.1” “%”. Medium should contain factors shown in the following table.

Conversion of human fibroblasts to angioblast-like progenitor cells

Mon, 01 Jan 0001 00:00:00 +0000

Conversion of human fibroblasts to angioblast-like progenitor cells

DOI: 10.1038/NMETH.2255

Authors: Leo Kurian; Ignacio Sancho-Martinez; Emmanuel Nivet; Aitor Aguirre; Krystal Moon; Caroline Pendaries; Cecile Volle-Challier; Francoise Bono; Jean-Marc Herbert; Julian Pulecio; Yun Xia; Mo Li; Nuria Montserrat; Sergio Ruiz; Ilir Dubova; Concepcion Rodriguez; Ahmet M Denli; Francesca S Boscolo; Rathi D Thiagarajan; Fred H Gage; Jeanne F Loring; Louise C Laurent; Juan Carlos Izpisua Belmonte

Step 1: iPSC/ESC maintainance

day 0 ~ 1

Cornea organoids from human induced pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

Cornea organoids from human induced pluripotent stem cells

DOI: 10.1038/srep41286

Authors: James W. Foster; Karl Wahlin; Sheila M. Adams; David E. Birk; Donald J. Zack; Shukti Chakravarti

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “spheroid” human PSC in “mTeSR”. Medium should contain factors shown in the following table.

	concentration	unit
blebbistatin	5.0e-6	mol/l

Ideally, “PSC” should be obtained at the end of culture.

Cyclic Strain Promotes H19 Expression and Vascular Tube Formation in iPSC-Derived Endothelial Cells

Mon, 01 Jan 0001 00:00:00 +0000

Cyclic Strain Promotes H19 Expression and Vascular Tube Formation in iPSC-Derived Endothelial Cells

DOI: 10.1007/s12195-020-00617-0

Authors: Mark J. Vander Roest; W. David Merryman

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “mTeSR” for “1” days. For ECM, use “Matrigel” at “0.1” “g/m2”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

	concentration	unit
Y27632	1.0e-5	mol/l

Ideally, “PSC” should be obtained at the end of culture.

Cyclosporin A toxicity on endothelial cells differentiated from induced pluripotent stem cells: Assembling an adverse outcome pathway

Mon, 01 Jan 0001 00:00:00 +0000

Cyclosporin A toxicity on endothelial cells differentiated from induced pluripotent stem cells: Assembling an adverse outcome pathway

DOI: 10.1016/j.tiv.2024.105954

Authors: Zahra Mazidi; Matthias Wieser; Nicoleta Spinu; Adelheid Weidinger; Andrey V. Kozlov; Kristijan Vukovic; Sara Wellens; Cormac Murphy; Pranika Singh; Liadys Mora Lagares; Madhusudhan Reddy Bobbili; Lisa Liendl; Markus Schosserer; Andreas Diendorfer; Bruno Bettelheim; Wolf Eilenberg; Thomas Exner; Maxime Culot; Paul Jennings; Anja Wilmes; Marjana Novic; Emilio Benfenati; Regina Grillari-Voglauer; Johannes Grillari

Cytokine-free directed differentiation of human pluripotent stem cells efficiently produces hemogenic endothelium with lymphoid potential

Mon, 01 Jan 0001 00:00:00 +0000

Cytokine-free directed differentiation of human pluripotent stem cells efficiently produces hemogenic endothelium with lymphoid potential

DOI: 10.1186/s13287-017-0519-0

Authors: Yekaterina Galat; Svetlana Dambaeva; Irina Elcheva; Aaruni Khanolkar; Kenneth Beaman; Philip M. Iannaccone; Vasiliy Galat

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “Matrigel” at “N/A” “N/A”.

Ideally, “PSC” should express “AP”, “TRA-1-80”, “NANOG”, “TRA-1-60”, “POU5F1” at the end of culture.

Defined MicroRNAs Induce Aspects of Maturation in Mouse and Human Embryonic-Stem-Cell-Derived Cardiomyocytes

Mon, 01 Jan 0001 00:00:00 +0000

Defined MicroRNAs Induce Aspects of Maturation in Mouse and Human Embryonic-Stem-Cell-Derived Cardiomyocytes

DOI: 10.1016/j.celrep.2015.08.042

Authors: Desy S. Lee; Jyh-Hong Chen; David J. Lundy; Chung-Hung Liu; Shiaw-Min Hwang; Lil Pabon; Ru-Chi Shieh; Chien-Chang Chen; Sheng-Nan Wu; Yu-Ting Yan; Sho-Tone Lee; Po-Min Chiang; Shu Chien; Charles E. Murry; Patrick C.H. Hsieh

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human ESC in “MEF-conditioned medium”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table.

Derivation of blood-brain barrier endothelial cells from human pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

Derivation of blood-brain barrier endothelial cells from human pluripotent stem cells

DOI: 10.1038/nbt.2247

Authors: Ethan S Lippmann; Samira M Azarin; Jennifer E Kay; Randy A Nessler; Hannah K Wilson; Abraham Al-Ahmad; Sean P Palecek; Eric V Shusta

Step 1: iPSC/ESC maintainance

day 0 ~ 2

culture “adherent” human PSC in “mTeSR” for “2” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should be changed once in “2” days.

Ideally, “PSC” should be obtained at the end of culture.

Derivation of Brain Capillary-like Endothelial Cells from Human Pluripotent Stem Cell-Derived Endothelial Progenitor Cells

Mon, 01 Jan 0001 00:00:00 +0000

Derivation of Brain Capillary-like Endothelial Cells from Human Pluripotent Stem Cell-Derived Endothelial Progenitor Cells

DOI: 10.1016/j.stemcr.2019.08.002

Authors: Catarina Praça; Susana C. Rosa; Emmanuel Sevin; Romeo Cecchelli; Marie-Pierre Dehouck; Lino S. Ferreira

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “unspecified medium”. For ECM, use “FN1” at “0.01” “g/m2”. Medium should contain factors shown in the following table.

	concentration	unit
collagenase IV	—	—

Step 2: mesoderm 1

day 1 ~ 11

Design of experiments for the automated development of a multicellular cardiac model for high-throughput screening

Mon, 01 Jan 0001 00:00:00 +0000

Design of experiments for the automated development of a multicellular cardiac model for high-throughput screening

DOI: 10.1016/j.slasd.2023.10.006

Authors: Kavita Raniga; William Stebbeds; Arun Shivalingam; Michelle Pemberton; Chris Denning

No steps available

Development of a multicellular in vitro model of the meningeal blood-CSF barrier to study Neisseria meningitidis infection

Mon, 01 Jan 0001 00:00:00 +0000

Development of a multicellular in vitro model of the meningeal blood-CSF barrier to study Neisseria meningitidis infection

DOI: 10.1186/s12987-022-00379-z

Authors: Leo M. Endres; Marvin Jungblut; Mustafa Divyapicigil; Markus Sauer; Christian Stigloher; Myron Christodoulides; Brandon J. Kim; Alexandra Schubert-Unkmeir

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “StemFlex medium” for “1” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should be changed once in “1” days.

Development of Human in vitro Brain-blood Barrier Model from Induced Pluripotent Stem Cell-derived Endothelial Cells to Predict the in vivo Permeability of Drugs

Mon, 01 Jan 0001 00:00:00 +0000

Development of Human in vitro Brain-blood Barrier Model from Induced Pluripotent Stem Cell-derived Endothelial Cells to Predict the in vivo Permeability of Drugs

DOI: 10.1007/s12264-019-00384-7

Authors: Yuan Li; Xueying Sun; Houfu Liu; Liang Huang; Guofeng Meng; Yu Ding; Wenji Su; Jiaqi Lu; Sophie Gong; Georg C. Terstappen; Ru Zhang; Wandong Zhang

Step 1: iPSC/ESC maintainance

day 0 ~ 2

culture “adherent” human PSC in “mTeSR” for “2” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should be changed once in “2” days.

Development of human iPSC-derived quiescent hepatic stellate cell-like cells for drug discovery and in vitro disease modeling

Mon, 01 Jan 0001 00:00:00 +0000

Development of human iPSC-derived quiescent hepatic stellate cell-like cells for drug discovery and in vitro disease modeling

DOI: 10.1016/j.stemcr.2021.11.002

Authors: Yuta Koui; Misao Himeno; Yusuke Mori; Yasuhiro Nakano; Eiko Saijou; Naoki Tanimizu; Yoshiko Kamiya; Hiroko Anzai; Natsuki Maeda; Luyao Wang; Tadanori Yamada; Yasuyuki Sakai; Ryuichiro Nakato; Atsushi Miyajima; Taketomo Kido

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “StemFlex Medium”. For ECM, use “Vitronectin” at “N/A” “N/A”.

Development of in vitro cardiovascular tissue models within capillary circuit microfluidic devices fabricated with 3D stereolithography printing

Mon, 01 Jan 0001 00:00:00 +0000

Development of in vitro cardiovascular tissue models within capillary circuit microfluidic devices fabricated with 3D stereolithography printing

DOI: 10.1007/s42452-023-05459-9

Authors: Aibhlin Esparza; Nicole Jimenez; Binata Joddar; Sylvia Natividad-Diaz

No steps available

Differential HDAC6 Activity Modulates Ciliogenesis and Subsequent Mechanosensing of Endothelial Cells Derived from Pluripotent Stem Cells

Mon, 01 Jan 0001 00:00:00 +0000

Differential HDAC6 Activity Modulates Ciliogenesis and Subsequent Mechanosensing of Endothelial Cells Derived from Pluripotent Stem Cells

DOI: 10.1016/j.celrep.2018.06.083

Authors: Quinton Smith; Bria Macklin; Xin Yi Chan; Hannah Jones; Michelle Trempel; Mervin C. Yoder; Sharon Gerecht

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “E8”. For ECM, use “Vitronectin” at “N/A” “N/A”.

Ideally, “PSC” should be obtained at the end of culture.

Differentiation and characterization of human iPSC-derived vascular endothelial cells under physiological shear stress

Mon, 01 Jan 0001 00:00:00 +0000

Differentiation and characterization of human iPSC-derived vascular endothelial cells under physiological shear stress

DOI: 10.1016/j.xpro.2021.100394

Authors: Nadia O. Abutaleb; George A. Truskey

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “mTeSR Plus” for “1” days. For ECM, use “Matrigel” at “1” “%”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

	concentration	unit
Y27632	1.0e-5	mol/l

Ideally, “PSC” should be obtained at the end of culture.

Differentiation and characterization of human pluripotent stem cell-derived brain microvascular endothelial cells

Mon, 01 Jan 0001 00:00:00 +0000

Differentiation and characterization of human pluripotent stem cell-derived brain microvascular endothelial cells

DOI: 10.1016/j.ymeth.2015.10.016

Authors: Matthew J. Stebbins; Hannah K. Wilson; Scott G. Canfield; Tongcheng Qian; Sean P. Palecek; Eric V. Shusta

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “mTeSR” for “1” days. For ECM, use “Matrigel” at “0.083” “g/l”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Differentiation and expansion of endothelial cells requires pre-optimization of KDR+ expression kinetics

Mon, 01 Jan 0001 00:00:00 +0000

Differentiation and expansion of endothelial cells requires pre-optimization of KDR+ expression kinetics

DOI: 10.1016/j.scr.2019.101685

Authors: Basharat Jahan; Kara E. McCloskey

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “E8” for “1” days. For ECM, use “Matrigel” at “0.2” “g/m2”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

	concentration	unit
Y27632	1.0e-8	mol/l

Ideally, “PSC” should be obtained at the end of culture.

Differentiation and Functional Comparison of Monocytes and Macrophages from hiPSCs with Peripheral Blood Derivatives

Mon, 01 Jan 0001 00:00:00 +0000

Differentiation and Functional Comparison of Monocytes and Macrophages from hiPSCs with Peripheral Blood Derivatives

DOI: 10.1016/j.stemcr.2019.05.003

Authors: Xu Cao; Gopala K. Yakala; Francijna E. van den Hil; Amy Cochrane; Christine L. Mummery; Valeria V. Orlova

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “E8” for “1” days. For ECM, use “Matrigel” at “0.075” “g/l”. Medium should be changed once in “1” days.

Ideally, “PSC” should be obtained at the end of culture.

Differentiation of Human Pluripotent Stem Cells into Functional Endothelial Cells in Scalable Suspension Culture

Mon, 01 Jan 0001 00:00:00 +0000

Differentiation of Human Pluripotent Stem Cells into Functional Endothelial Cells in Scalable Suspension Culture

DOI: 10.1016/j.stemcr.2018.03.017

Authors: Ruth Olmer; Lena Engels; Abdulai Usman; Sandra Menke; Muhammad Nasir Hayat Malik; Frank Pessler; Gudrun Göhring; Dorothee Bornhorst; Svenja Bolten; Salim Abdelilah-Seyfried; Thomas Scheper; Henning Kempf; Robert Zweigerdt; Ulrich Martin

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “spheroid” human PSC in “unspecified medium”.

Step 2: mesoderm 1

day 1 ~ 3

Differentiation of human pluripotent stem cells to brain microvascular endothelial cell-like cells suitable to study immune cell interactions

Mon, 01 Jan 0001 00:00:00 +0000

Differentiation of human pluripotent stem cells to brain microvascular endothelial cell-like cells suitable to study immune cell interactions

DOI: 10.1016/j.xpro.2021.100563

Authors: Hideaki Nishihara; Benjamin D. Gastfriend; Pelin Kasap; Sean P. Palecek; Eric V. Shusta; Britta Engelhardt

Step 1: iPSC/ESC maintainance

day 0 ~ 1

Direct programming of human pluripotent stem cells into endothelial progenitors with SOX17 and FGF2

Mon, 01 Jan 0001 00:00:00 +0000

Direct programming of human pluripotent stem cells into endothelial progenitors with SOX17 and FGF2

DOI: 10.1016/j.stemcr.2024.02.006

Authors: Michael W. Ream; Lauren N. Randolph; Yuqian Jiang; Yun Chang; Xiaoping Bao; Xiaojun Lance Lian

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “E8”. Medium should contain factors shown in the following table.

	concentration	unit
blasticidin	0.01	g/l

Step 2: endothelial 1

day 1 ~ 2

Direct reprogramming of epithelial cell rests of malassez into mesenchymal-like cells by epigenetic agents

Mon, 01 Jan 0001 00:00:00 +0000

Direct reprogramming of epithelial cell rests of malassez into mesenchymal-like cells by epigenetic agents

DOI: 10.1038/s41598-020-79426-4

Authors: Koki Yoshida; Osamu Uehara; Yoshihito Kurashige; Durga Paudel; Aya Onishi; Puja Neopane; Daichi Hiraki; Tetsuro Morikawa; Fumiya Harada; Rie Takai; Jun Sato; Masato Saitoh; Yoshihiro Abiko

Step 1: iPSC/ESC maintainance

day 0 ~ 14

culture “adherent” porcine ERM in “Primate ES medium” for “14” days. Medium should contain factors shown in the following table, and should be changed once in “14” days.

Direct Reprogramming of Murine Fibroblasts to Hematopoietic Progenitor Cells

Mon, 01 Jan 0001 00:00:00 +0000

Direct Reprogramming of Murine Fibroblasts to Hematopoietic Progenitor Cells

DOI: 10.1016/j.celrep.2014.11.002

Authors: Kiran Batta; Magdalena Florkowska; Valerie Kouskoff; Georges Lacaud

Step 1: endothelial 1

day 0 ~ 5

culture “adherent” mouse Fibroblast in “IMDM” for “5” days. For ECM, use “Gelatin” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “5” days.

	concentration	unit
PDS	—	—
PFM	10	%
Ascorbic Acid	5.0e-4	mol/l
MTG	4.5e-4	mol/l
Glutamine	0.002	mol/l
Transferrin	80	g/l
SCF	1	%
IL-3	1	%
GM-CSF	1	%
TPO	1	%
EPO	4000	U/l
M-CSF	1.0e-5	g/l
IL-6	1.0e-5	g/l
IL-11	5.0e-6	g/l
PSA	0.05	g/l
diethylaminoethyl dextran	0.01	g/l

Ideally, “EC” should express “CDH5”, “TEK”, “Icam1”, “PECAM1”, “Vwf” at the end of culture.

Directed differentiation and long-term maintenance of epicardial cells derived from human pluripotent stem cells under fully defined conditions

Mon, 01 Jan 0001 00:00:00 +0000

Directed differentiation and long-term maintenance of epicardial cells derived from human pluripotent stem cells under fully defined conditions

DOI: 10.1038/nprot.2017.080

Authors: Xiaoping Bao; Xiaojun Lian; Tongcheng Qian; Vijesh J Bhute; Tianxiao Han; Sean P Palecek

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “E8” for “1” days. For ECM, use “Synthemax” at “0.025” “g/l”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Disease Model of GATA4 Mutation Reveals Transcription Factor Cooperativity in Human Cardiogenesis

Mon, 01 Jan 0001 00:00:00 +0000

Disease Model of GATA4 Mutation Reveals Transcription Factor Cooperativity in Human Cardiogenesis

DOI: 10.1016/j.cell.2016.11.033

Authors: Yen-Sin Ang; Renee N. Rivas; Alexandre J.S. Ribeiro; Rohith Srivas; Janell Rivera; Nicole R. Stone; Karishma Pratt; Tamer M.A. Mohamed; Ji-Dong Fu; C. Ian Spencer; Nathaniel D. Tippens; Molong Li; Anil Narasimha; Ethan Radzinsky; Anita J. Moon-Grady; Haiyuan Yu; Beth L. Pruitt; Michael P. Snyder; Deepak Srivastava

Step 1: iPSC/ESC maintainance

day 0 ~ 3

culture “adherent” human PSC in “mTeSR” for “3” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “3” days.

Downregulation of LGR5 Expression Inhibits Cardiomyocyte Differentiation and Potentiates Endothelial Differentiation from Human Pluripotent Stem Cells

Mon, 01 Jan 0001 00:00:00 +0000

Downregulation of LGR5 Expression Inhibits Cardiomyocyte Differentiation and Potentiates Endothelial Differentiation from Human Pluripotent Stem Cells

DOI: 10.1016/j.stemcr.2017.07.006

Authors: Rajneesh Jha; Monalisa Singh; Qingling Wu; Cinsley Gentillon; Marcela K. Preininger; Chunhui Xu

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “MEF-CM” for “1” days. “PSC” should be “downregulated” for “LGR5”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Dual effect of TAT functionalized DHAH lipid nanoparticles with neurotrophic factors in human BBB and microglia cultures

Mon, 01 Jan 0001 00:00:00 +0000

Dual effect of TAT functionalized DHAH lipid nanoparticles with neurotrophic factors in human BBB and microglia cultures

DOI: 10.1186/s12987-022-00315-1

Authors: Sara Hernando; Polyxeni Nikolakopoulou; Dimitrios Voulgaris; Rosa Maria Hernandez; Manoli Igartua; Anna Herland

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “mTeSR” for “1” days. For ECM, use “Matrigel” at “8.3e-5” “g/well plate”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Dynamic chromatin organization and regulatory interactions in human endothelial cell differentiation

Mon, 01 Jan 0001 00:00:00 +0000

Dynamic chromatin organization and regulatory interactions in human endothelial cell differentiation

DOI: 10.1016/j.stemcr.2022.11.003

Authors: Kris G. Alavattam; Katie A. Mitzelfelt; Giancarlo Bonora; Paul A. Fields; Xiulan Yang; Han Sheng Chiu; Lil Pabon; Alessandro Bertero; Nathan J. Palpant; William S. Noble; Charles E. Murry

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” human ESC in “unspecified medium”.

Ideally, “ESC” should be obtained at the end of culture.

Effect of biochemical and biomechanical factors on vascularization of kidney organoid-on-a-chip

Mon, 01 Jan 0001 00:00:00 +0000

Effect of biochemical and biomechanical factors on vascularization of kidney organoid-on-a-chip

DOI: 10.1186/s40580-021-00285-4

Authors: Han Na Lee; Yoon Young Choi; Jin Won Kim; Young Seo Lee; Ji Wook Choi; Taewook Kang; Yong Kyun Kim; Bong Guen Chung

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “Matrigel” at “3” “%”. Medium should contain factors shown in the following table.

Effect of culture medium on propagation and phenotype of corneal stroma–derived stem cells

Mon, 01 Jan 0001 00:00:00 +0000

Effect of culture medium on propagation and phenotype of corneal stroma–derived stem cells

DOI: 10.1016/j.jcyt.2015.08.003

Authors: Laura E. Sidney; Matthew J. Branch; Harminder S. Dua; Andrew Hopkinson

No steps available

Effect of Human Donor Cell Source on Differentiation and Function of Cardiac Induced Pluripotent Stem Cells

Mon, 01 Jan 0001 00:00:00 +0000

Effect of Human Donor Cell Source on Differentiation and Function of Cardiac Induced Pluripotent Stem Cells

DOI: 10.1016/j.jacc.2014.04.056

Authors: Veronica Sanchez-Freire; Andrew S. Lee; Shijun Hu; Oscar J. Abilez; Ping Liang; Feng Lan; Bruno C. Huber; Sang-Ging Ong; Wan Xing Hong; Mei Huang; Joseph C. Wu

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “unspecified medium”. For ECM, use “Matrigel” at “N/A” “N/A”.

Effective endothelial cell and human pluripotent stem cell interactions generate functional insulin-producing beta cells

Mon, 01 Jan 0001 00:00:00 +0000

Effective endothelial cell and human pluripotent stem cell interactions generate functional insulin-producing beta cells

DOI: 10.1007/s00125-016-4078-1

Authors: Dodanim Talavera-Adame; Orison O. Woolcott; Joseph Ignatius-Irudayam; Vaithilingaraja Arumugaswami; David H. Geller; Donald C. Dafoe

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” human PSC in “mTeSR”. Medium should contain factors shown in the following table.

	concentration	unit
L-alanyl-L-glutamine	2.0e-4	mol/l
2-mercaptoethanol	1.0e-4	mol/l

Ideally, “PSC” should be obtained at the end of culture.

Efficient and Reproducible Differentiation Protocol for Pluripotent Stem Cells into Functional Endothelial Cells: Unveiling the Path to Vascular Regeneration

Mon, 01 Jan 0001 00:00:00 +0000

Efficient and Reproducible Differentiation Protocol for Pluripotent Stem Cells into Functional Endothelial Cells: Unveiling the Path to Vascular Regeneration

DOI: 10.1016/j.arcmed.2024.103142

Authors: Manizheh Azhdari; Axel zur Hausen; Nasser Aghdami; Mohamadreza Baghaban-Eslaminejad

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “DMEM/F12”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table.

	concentration	unit
Glutamine	0.002	mol/l
NEAA	1	%
2-mercaptoethanol	1.0e-4	mol/l
KSR	10	%
PSA	100000	IU/l
ITS	—	—
streptomycin	100	g/l
FGF	1.0e-4	g/l

Ideally, “PSC” should be obtained at the end of culture.

Efficient differentiation and purification of human induced pluripotent stem cell-derived endothelial progenitor cells and expansion with the use of inhibitors of ROCK, TGF-β, and GSK3β

Mon, 01 Jan 0001 00:00:00 +0000

Efficient differentiation and purification of human induced pluripotent stem cell-derived endothelial progenitor cells and expansion with the use of inhibitors of ROCK, TGF-β, and GSK3β

DOI: 10.1016/j.heliyon.2020.e03493

Authors: Hiromasa Aoki; Misaki Yamashita; Tadahiro Hashita; Koichi Ogami; Shinichi Hoshino; Takahiro Iwao; Tamihide Matsunaga

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “Essential 8 Flex medium” for “1” days. For ECM, use “VTN-N” at “0.01” “g/m2”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Efficient Differentiation of Human Pluripotent Stem Cells to Endothelial Progenitors via Small-Molecule Activation of WNT Signaling

Mon, 01 Jan 0001 00:00:00 +0000

Efficient Differentiation of Human Pluripotent Stem Cells to Endothelial Progenitors via Small-Molecule Activation of WNT Signaling

DOI: 10.1016/j.stemcr.2014.09.005

Authors: Xiaojun Lian; Xiaoping Bao; Abraham Al-Ahmad; Jialu Liu; Yue Wu; Wentao Dong; Kaitlin K. Dunn; Eric V. Shusta; Sean P. Palecek

Step 1: iPSC/ESC maintainance

day 0 ~ 1

Efficient Direct Reprogramming of Mature Amniotic Cells into Endothelial Cells by ETS Factors and TGFβ Suppression

Mon, 01 Jan 0001 00:00:00 +0000

Efficient Direct Reprogramming of Mature Amniotic Cells into Endothelial Cells by ETS Factors and TGFβ Suppression

DOI: 10.1016/j.cell.2012.09.032

Authors: Michael Ginsberg; Daylon James; Bi-Sen Ding; Daniel Nolan; Fuqiang Geng; Jason M. Butler; William Schachterle; Venkat R. Pulijaal; Susan Mathew; Stephen T. Chasen; Jenny Xiang; Zev Rosenwaks; Koji Shido; Olivier Elemento; Sina Y. Rabbany; Shahin Rafii

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human ESC in “KOSR medium”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table.

Efficient generation of human NOTCH ligand-expressing haemogenic endothelial cells as infrastructure for in vitro haematopoiesis and lymphopoiesis

Mon, 01 Jan 0001 00:00:00 +0000

Efficient generation of human NOTCH ligand-expressing haemogenic endothelial cells as infrastructure for in vitro haematopoiesis and lymphopoiesis

DOI: 10.1038/s41467-024-51974-7

Authors: Shicheng Sun; Ali Motazedian; Jacky Y. Li; Kevin Wijanarko; Joe Jiang Zhu; Kothila Tharmarajah; Kathleen A. Strumila; Anton Shkaruta; L. Rayburn Nigos; Jacqueline V. Schiesser; Yi Yu; Paul J. Neeson; Elizabeth S. Ng; Andrew G. Elefanty; Edouard G. Stanley

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “E8” for “1” days. For ECM, use “Geltrex” at “1” “%”. Medium should be changed once in “1” days.

Efficient generation of liver sinusoidal endothelial-like cells secreting coagulation factor VIII from human induced pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

Efficient generation of liver sinusoidal endothelial-like cells secreting coagulation factor VIII from human induced pluripotent stem cells

DOI: 10.1016/j.omtm.2024.101355

Authors: Seiji Mitani; Chihiro Hosoda; Yu Onodera; Yoko Takabayashi; Asuka Sakata; Midori Shima; Kohei Tatsumi

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “StemFit AK02N”. For ECM, use “Laminin” at “N/A” “N/A”.

Ideally, “PSC” should be obtained at the end of culture.

Efficient long-term survival of cell grafts after myocardial infarction with thick viable cardiac tissue entirely from pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

Efficient long-term survival of cell grafts after myocardial infarction with thick viable cardiac tissue entirely from pluripotent stem cells

DOI: 10.1038/srep16842

Authors: Takehiko Matsuo; Hidetoshi Masumoto; Shuhei Tajima; Takeshi Ikuno; Shiori Katayama; Kenji Minakata; Tadashi Ikeda; Kohei Yamamizu; Yasuhiko Tabata; Ryuzo Sakata; Jun K. Yamashita

Step 1: mesoderm 1

day 0 ~ 4

culture “adherent” mouse ESC in “alpha-MEM” for “4” days. For ECM, use “Gelatin” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “4” days.

Endothelial and hematopoietic hPSCs differentiation via a hematoendothelial progenitor

Mon, 01 Jan 0001 00:00:00 +0000

Endothelial and hematopoietic hPSCs differentiation via a hematoendothelial progenitor

DOI: 10.1186/s13287-022-02925-w

Authors: Alejandra Vargas-Valderrama; Anne-Charlotte Ponsen; Morgane Le Gall; Denis Clay; Sébastien Jacques; Tudor Manoliu; Valérie Rouffiac; Karine Ser-le-Roux; Cyril Quivoron; Fawzia Louache; Georges Uzan; Maria-Teresa Mitjavila-Garcia; Estelle Oberlin; Hind Guenou

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “KnockOut DMEM” for “1” days. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Endothelial precursor cell-based therapy to target the pathologic angiogenesis and compensate tumor hypoxia

Mon, 01 Jan 0001 00:00:00 +0000

Endothelial precursor cell-based therapy to target the pathologic angiogenesis and compensate tumor hypoxia

DOI: 10.1016/j.canlet.2015.11.008

Authors: Guillaume Collet; Krzysztof Szade; Witold Nowak; Krzysztof Klimkiewicz; Bouchra El Hafny-Rahbi; Karol Szczepanek; Daisuke Sugiyama; Kazimierz Weglarczyk; Alexandra Foucault-Collet; Alan Guichard; Andrzej Mazan; Mahdi Nadim; Fabienne Fasani; Nathalie Lamerant-Fayel; Catherine Grillon; Stéphane Petoud; Jean-Claude Beloeil; Alicja Jozkowicz; Jozef Dulak; Claudine Kieda

No steps available

Endothelial progenitor cells and neural progenitor cells synergistically protect cerebral endothelial cells from Hypoxia/reoxygenation-induced injury via activating the PI3K/Akt pathway

Mon, 01 Jan 0001 00:00:00 +0000

Endothelial progenitor cells and neural progenitor cells synergistically protect cerebral endothelial cells from Hypoxia/reoxygenation-induced injury via activating the PI3K/Akt pathway

DOI: 10.1186/s13041-016-0193-7

Authors: Jinju Wang; Yusen Chen; Yi Yang; Xiang Xiao; Shuzhen Chen; Cheng Zhang; Bradley Jacobs; Bin Zhao; Ji Bihl; Yanfang Chen

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “Matrigel” at “N/A” “N/A”.

Engineered myocardium model to study the roles of HIF-1α and HIF1A-AS1 in paracrine-only signaling under pathological level oxidative stress

Mon, 01 Jan 0001 00:00:00 +0000

Engineered myocardium model to study the roles of HIF-1α and HIF1A-AS1 in paracrine-only signaling under pathological level oxidative stress

DOI: 10.1016/j.actbio.2017.06.023

Authors: Aylin Acun; Pinar Zorlutuna

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “Geltrex” at “N/A” “N/A”.

Step 2: mesoderm 1

day 1 ~ 4

culture “adherent” human PSC in “N2B27 medium” for “3” days. For ECM, use “Geltrex” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “3” days.

Engineering hiPSC-CM and hiPSC-EC laden 3D nanofibrous splenic hydrogel for improving cardiac function through revascularization and remuscularization in infarcted heart

Mon, 01 Jan 0001 00:00:00 +0000

Engineering hiPSC-CM and hiPSC-EC laden 3D nanofibrous splenic hydrogel for improving cardiac function through revascularization and remuscularization in infarcted heart

DOI: 10.1016/j.bioactmat.2021.04.010

Authors: Ge Guan; Da Huo; Yanzhao Li; Xiaolin Zhao; Yinghao Li; Zhongliang Qin; Dayu Sun; Guanyuan Yang; Mingcan Yang; Ju Tan; Wen Zeng; Chuhong Zhu

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “mTeSR” for “1” days. For ECM, use “SpGel” at “N/A” “N/A”. Medium should be changed once in “1” days.

Engineering the haemogenic niche mitigates endogenous inhibitory signals and controls pluripotent stem cell-derived blood emergence

Mon, 01 Jan 0001 00:00:00 +0000

Engineering the haemogenic niche mitigates endogenous inhibitory signals and controls pluripotent stem cell-derived blood emergence

DOI: 10.1038/ncomms15380

Authors: Nafees Rahman; Patrick M. Brauer; Lilian Ho; Tatiana Usenko; Mukul Tewary; Juan Carlos Zúñiga-Pflücker; Peter W. Zandstra

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human ESC in “DMEM/F12” for “1” days. “ESC” should be “unspecified regulation” for “RUNX1C”. For ECM, use “Fibroblast” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Engraftment and morphological development of vascularized human iPS cell-derived 3D-cardiomyocyte tissue after xenotransplantation

Mon, 01 Jan 0001 00:00:00 +0000

Engraftment and morphological development of vascularized human iPS cell-derived 3D-cardiomyocyte tissue after xenotransplantation

DOI: 10.1038/s41598-017-14053-0

Authors: Hirokazu Narita; Fumiaki Shima; Junya Yokoyama; Shigeru Miyagawa; Yoshinari Tsukamoto; Yasushi Takamura; Ayami Hiura; Ken Fukumoto; Tomohiro Chiba; Seiji Watanabe; Yoshiki Sawa; Mitsuru Akashi; Hiroshi Shimoda

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “device” human PSC in “E8” for “1” days. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Engraftment of human induced pluripotent stem cell-derived hepatocytes in immunocompetent mice via 3D co-aggregation and encapsulation

Mon, 01 Jan 0001 00:00:00 +0000

Engraftment of human induced pluripotent stem cell-derived hepatocytes in immunocompetent mice via 3D co-aggregation and encapsulation

DOI: 10.1038/srep16884

Authors: Wei Song; Yen-Chun Lu; Angela S. Frankel; Duo An; Robert E. Schwartz; Minglin Ma

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “unspecified medium”. For ECM, use “Matrigel” at “N/A” “N/A”.

Ideally, “PSC” should be obtained at the end of culture.

Enhanced HSC-like cell generation from mouse pluripotent stem cells in a 3D induction system cocultured with stromal cells

Mon, 01 Jan 0001 00:00:00 +0000

Enhanced HSC-like cell generation from mouse pluripotent stem cells in a 3D induction system cocultured with stromal cells

DOI: 10.1186/s13287-021-02434-2

Authors: Wei Shan; Qin Yu; Yan Long; Qian Luo; Honghu Li; Yingli Han; Yulin Xu; Shan Fu; Xiangjun Zeng; Cong Wei; Yang Gao; Xiaoqing Li; Xia Li; Lifei Zhang; Lizhen Liu; Ming Chen; Pengxu Qian; He Huang

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

Enhanced Lung Epithelial Specification of Human Induced Pluripotent Stem Cells on Decellularized Lung Matrix

Mon, 01 Jan 0001 00:00:00 +0000

Enhanced Lung Epithelial Specification of Human Induced Pluripotent Stem Cells on Decellularized Lung Matrix

DOI: 10.1016/j.athoracsur.2014.05.080

Authors: Sarah E. Gilpin; Xi Ren; Tatsuya Okamoto; Jacques P. Guyette; Hongmei Mou; Jayaraj Rajagopal; Douglas J. Mathisen; Joseph P. Vacanti; Harald C. Ott

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “Geltrex” at “N/A” “N/A”.

Ideally, “PSC” should be obtained at the end of culture.

Enhanced xeno-free differentiation of hiPSC-derived astroglia applied in a blood–brain barrier model

Mon, 01 Jan 0001 00:00:00 +0000

Enhanced xeno-free differentiation of hiPSC-derived astroglia applied in a blood–brain barrier model

DOI: 10.1186/s12987-019-0147-4

Authors: Louise Delsing; Therése Kallur; Henrik Zetterberg; Ryan Hicks; Jane Synnergren

Step 1: endothelial 1

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” unknown species cell and human EC in “unspecified medium”. Medium should contain factors shown in the following table.

	concentration	unit
serum	—	—

Ideally, “EC” should be obtained at the end of culture.

Enhancement of airway epithelial cell differentiation by pulmonary endothelial cell co-culture

Mon, 01 Jan 0001 00:00:00 +0000

Enhancement of airway epithelial cell differentiation by pulmonary endothelial cell co-culture

DOI: 10.1016/j.scr.2022.102967

Authors: Umida Burkhanova; Ann Harris; Shih-Hsing Leir

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” human PSC in “unspecified medium”.

Ideally, “PSC” should be obtained at the end of culture.

Epigenetic landscape reveals MECOM as an endothelial lineage regulator

Mon, 01 Jan 0001 00:00:00 +0000

Epigenetic landscape reveals MECOM as an endothelial lineage regulator

DOI: 10.1038/s41467-023-38002-w

Authors: Jie Lv; Shu Meng; Qilin Gu; Rongbin Zheng; Xinlei Gao; Jun-dae Kim; Min Chen; Bo Xia; Yihan Zuo; Sen Zhu; Dongyu Zhao; Yanqiang Li; Guangyu Wang; Xin Wang; Qingshu Meng; Qi Cao; John P. Cooke; Longhou Fang; Kaifu Chen; Lili Zhang

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “mTeSR” for “1” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should be changed once in “1” days.

Establishment of a translational endothelial cell model using directed differentiation of induced pluripotent stem cells from Cynomolgus monkey

Mon, 01 Jan 0001 00:00:00 +0000

Establishment of a translational endothelial cell model using directed differentiation of induced pluripotent stem cells from Cynomolgus monkey

DOI: 10.1038/srep35830

Authors: Eva C. Thoma; Tobias Heckel; David Keller; Nicolas Giroud; Brian Leonard; Klaus Christensen; Adrian Roth; Cristina Bertinetti-Lapatki; Martin Graf; Christoph Patsch

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” Cynomolgus monkey PSC in “MT medium” for “1” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Evaluating the potential of endothelial cells derived from human induced pluripotent stem cells to form microvascular networks in 3D cultures

Mon, 01 Jan 0001 00:00:00 +0000

Evaluating the potential of endothelial cells derived from human induced pluripotent stem cells to form microvascular networks in 3D cultures

DOI: 10.1038/s41598-018-20966-1

Authors: Jonathan R. Bezenah; Yen P. Kong; Andrew J. Putnam

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” human PSC in “unspecified medium”.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: endothelial 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

Evaluation of a human iPSC-derived BBB model for repeated dose toxicity testing with cyclosporine A as model compound

Mon, 01 Jan 0001 00:00:00 +0000

Evaluation of a human iPSC-derived BBB model for repeated dose toxicity testing with cyclosporine A as model compound

DOI: 10.1016/j.tiv.2021.105112

Authors: Sara Wellens; Lucie Dehouck; Vidya Chandrasekaran; Pranika Singh; Rodrigo Azevedo Loiola; Emmanuel Sevin; Thomas Exner; Paul Jennings; Fabien Gosselet; Maxime Culot

Step 1: iPSC/ESC maintainance

day 0 ~ 1

Evaluation of the impact of iPSC differentiation protocols on transcriptomic signatures

Mon, 01 Jan 0001 00:00:00 +0000

Evaluation of the impact of iPSC differentiation protocols on transcriptomic signatures

DOI: 10.1016/j.tiv.2024.105826

Authors: Vidya Chandrasekaran; Sara Wellens; Aurore Bourguignon; Ivo Djidrovski; Leonie Fransen; Sreya Ghosh; Zahra Mazidi; Cormac Murphy; Carolina Nunes; Pranika Singh; Melinda Zana; Lyle Armstrong; András Dinnyés; Johannes Grillari; Regina Grillari-Voglauer; Martin O. Leonard; Catherine Verfaillie; Anja Wilmes; Marie-Gabrielle Zurich; Thomas Exner; Paul Jennings; Maxime Culot

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

Exosomes secreted by endothelial cells derived from human induced pluripotent stem cells improve recovery from myocardial infarction in mice

Mon, 01 Jan 0001 00:00:00 +0000

Exosomes secreted by endothelial cells derived from human induced pluripotent stem cells improve recovery from myocardial infarction in mice

DOI: 10.1186/s13287-023-03462-w

Authors: Hao Li; Lu Wang; Teng Ma; Zhongmin Liu; Ling Gao

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” human PSC in “unspecified medium”.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: endothelial 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

Exosomes secreted by human-induced pluripotent stem cell-derived mesenchymal stem cells attenuate limb ischemia by promoting angiogenesis in mice

Mon, 01 Jan 0001 00:00:00 +0000

Exosomes secreted by human-induced pluripotent stem cell-derived mesenchymal stem cells attenuate limb ischemia by promoting angiogenesis in mice

DOI: 10.1186/scrt546

Authors: Guo-wen Hu; Qing Li; Xin Niu; Bin Hu; Juan Liu; Shu-min Zhou; Shang-chun Guo; Hai-li Lang; Chang-qing Zhang; Yang Wang; Zhi-feng Deng

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “Matrigel” at “N/A” “N/A”.

Expandable hESC-derived cardiovascular progenitor cells generate functional cardiac lineage cells for microtissue construction

Mon, 01 Jan 0001 00:00:00 +0000

Expandable hESC-derived cardiovascular progenitor cells generate functional cardiac lineage cells for microtissue construction

DOI: 10.1186/s13287-024-03919-6

Authors: Siamak Rezaeiani; Malihe Rezaee; Mojtaba Shafaghi; Mohammad Karami; Roghayeh Hamidi; Hamid Khodayari; Sadaf Vahdat; Sara Pahlavan; Hossein Baharvand

Step 1: iPSC/ESC maintainance

day 0 ~ 5

culture “spheroid” human ESC in “unspecified medium” for “5” days. Medium should be changed once in “5” days.

Step 2: mesoderm 1

day 5 ~ 6

culture “spheroid” human ESC in “unspecified medium” for “1” days. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Exploring dysfunctional barrier phenotypes associated with glaucoma using a human pluripotent stem cell-based model of the neurovascular unit

Mon, 01 Jan 0001 00:00:00 +0000

Exploring dysfunctional barrier phenotypes associated with glaucoma using a human pluripotent stem cell-based model of the neurovascular unit

DOI: 10.1186/s12987-024-00593-x

Authors: Sailee S. Lavekar; Jason M. Hughes; Cátia Gomes; Kang-Chieh Huang; Jade Harkin; Scott G. Canfield; Jason S. Meyer

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “mTeSR” for “1” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should be changed once in “1” days.

Extracellular vesicles from human embryonic stem cell-derived cardiovascular progenitor cells promote cardiac infarct healing through reducing cardiomyocyte death and promoting angiogenesis

Mon, 01 Jan 0001 00:00:00 +0000

Extracellular vesicles from human embryonic stem cell-derived cardiovascular progenitor cells promote cardiac infarct healing through reducing cardiomyocyte death and promoting angiogenesis

DOI: 10.1038/s41419-020-2508-y

Authors: Qiang Wu; Jinxi Wang; Wilson Lek Wen Tan; Yun Jiang; Shihui Wang; Qiang Li; Xiujian Yu; Jiliang Tan; Shenyan Liu; Peng Zhang; Zenia Tiang; Zhongyan Chen; Roger Sik-Yin Foo; Huang-Tian Yang

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human ESC in “mTeSR”. For ECM, use “Matrigel” at “N/A” “N/A”.

Fabricating 3-dimensional human brown adipose microtissues for transplantation studies

Mon, 01 Jan 0001 00:00:00 +0000

Fabricating 3-dimensional human brown adipose microtissues for transplantation studies

DOI: 10.1016/j.bioactmat.2022.10.022

Authors: Ou Wang; Li Han; Haishuang Lin; Mingmei Tian; Shuyang Zhang; Bin Duan; Soonkyu Chung; Chi Zhang; Xiaojun Lian; Yong Wang; Yuguo Lei

No steps available

Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells

DOI: 10.1016/j.isci.2023.108599

Authors: Clifford Z. Liu; Aditi Prasad; Bharati Jadhav; Yu Liu; Mingxia Gu; Andrew J. Sharp; Bruce D. Gelb

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “mTeSR Plus” for “1” days. For ECM, use “Matrigel” at “6.6” “%”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Fetal sheep support the development of hematopoietic cells in vivo from human induced pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

Fetal sheep support the development of hematopoietic cells in vivo from human induced pluripotent stem cells

DOI: 10.1016/j.exphem.2020.12.006

Authors: Tomoyuki Abe; Hideki Uosaki; Hiroaki Shibata; Hiromasa Hara; Borjigin Sarentonglaga; Yoshikazu Nagao; Yutaka Hanazono

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “Primate ES Cell Medium”. For ECM, use “MEF feeder” at “N/A” “N/A”. Medium should contain factors shown in the following table.

FLI1 and PKC co-activation promote highly efficient differentiation of human embryonic stem cells into endothelial-like cells

Mon, 01 Jan 0001 00:00:00 +0000

FLI1 and PKC co-activation promote highly efficient differentiation of human embryonic stem cells into endothelial-like cells

DOI: 10.1038/s41419-017-0162-9

Authors: Hao Zhao; Yan Zhao; Zili Li; Qi Ouyang; Yi Sun; Di Zhou; Pingyuan Xie; Sicong Zeng; Lingfeng Dong; Hua Wen; Guangxiu Lu; Ge Lin; Liang Hu

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “mTeSR” for “1” days. “PSC” should be “unspecified regulation” for “FLI1”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Functional characterization of iPSC-derived arterial- and venous-like endothelial cells

Mon, 01 Jan 0001 00:00:00 +0000

Functional characterization of iPSC-derived arterial- and venous-like endothelial cells

DOI: 10.1038/s41598-019-40417-9

Authors: S. Rosa; C. Praça; P. R. Pitrez; P. José Gouveia; X. L. Aranguren; L. Ricotti; L. Silva Ferreira

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “CDM”. For ECM, use “FN1” at “N/A” “N/A”. Medium should contain factors shown in the following table.

	concentration	unit
BSA	5	g/l
insulin	7	g/l
Transferrin	15	g/l
PSA	50000	U/l

Step 2: mesoderm 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

Functional Vascular Endothelium Derived from Human Induced Pluripotent Stem Cells

Mon, 01 Jan 0001 00:00:00 +0000

Functional Vascular Endothelium Derived from Human Induced Pluripotent Stem Cells

DOI: 10.1016/j.stemcr.2013.06.007

Authors: William J. Adams; Yuzhi Zhang; Jennifer Cloutier; Pranati Kuchimanchi; Gail Newton; Seema Sehrawat; William C. Aird; Tanya N. Mayadas; Francis W. Luscinskas; Guillermo García-Cardeña

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” human PSC in “unspecified medium”.

Step 2: endothelial 1

day 1 ~ 11

culture “spheroid” human PSC in “unspecified medium” for “10” days. Medium should contain factors shown in the following table, and should be changed once in “10” days.

GATA2 Is Dispensable for Specification of Hemogenic Endothelium but Promotes Endothelial-to-Hematopoietic Transition

Mon, 01 Jan 0001 00:00:00 +0000

GATA2 Is Dispensable for Specification of Hemogenic Endothelium but Promotes Endothelial-to-Hematopoietic Transition

DOI: 10.1016/j.stemcr.2018.05.002

Authors: HyunJun Kang; Walatta-Tseyon Mesquitta; Ho Sun Jung; Oleg V. Moskvin; James A. Thomson; Igor I. Slukvin

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human ESC in “E8”. “ESC” should be “downregulated” for “GATA2”. For ECM, use “Matrigel” at “N/A” “N/A”.

Ideally, “ESC” should be obtained at the end of culture.

GATA2 Promotes Hematopoietic Development and Represses Cardiac Differentiation of Human Mesoderm

Mon, 01 Jan 0001 00:00:00 +0000

GATA2 Promotes Hematopoietic Development and Represses Cardiac Differentiation of Human Mesoderm

DOI: 10.1016/j.stemcr.2019.07.009

Authors: Julio Castaño; Sergi Aranda; Clara Bueno; Fernando J. Calero-Nieto; Eva Mejia-Ramirez; Jose Luis Mosquera; Enrique Blanco; Xiaonan Wang; Cristina Prieto; Lorea Zabaleta; Elisabetta Mereu; Meritxell Rovira; Senda Jiménez-Delgado; Daniel R. Matson; Holger Heyn; Emery H. Bresnick; Berthold Göttgens; Luciano Di Croce; Pablo Menendez; Angel Raya; Alessandra Giorgetti

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “mTeSR” for “1” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should be changed once in “1” days.

Generating high-purity cardiac and endothelial derivatives from patterned mesoderm using human pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

Generating high-purity cardiac and endothelial derivatives from patterned mesoderm using human pluripotent stem cells

DOI: 10.1038/nprot.2016.153

Authors: Nathan J Palpant; Lil Pabon; Clayton E Friedman; Meredith Roberts; Brandon Hadland; Rebecca J Zaunbrecher; Irwin Bernstein; Ying Zheng; Charles E Murry

Step 1: iPSC/ESC maintainance

day 0 ~ 1

Generation and characterisation of scalable and stable human pluripotent stem cell-derived microvascular-like endothelial cells for cardiac applications

Mon, 01 Jan 0001 00:00:00 +0000

Generation and characterisation of scalable and stable human pluripotent stem cell-derived microvascular-like endothelial cells for cardiac applications

DOI: 10.1007/s10456-024-09929-5

Authors: Qasim A. Majid; Bishwa R. Ghimire; Bela Merkely; Anna M. Randi; Sian E. Harding; Virpi Talman; Gábor Földes

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human ESC in “mTeSR” for “1” days. For ECM, use “Matrigel” at “2” “%”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Generation and characterization of a control and patient-derived human iPSC line containing the Hermansky Pudlak type 2 (HPS2) associated heterozygous compound mutation in AP3B1

Mon, 01 Jan 0001 00:00:00 +0000

Generation and characterization of a control and patient-derived human iPSC line containing the Hermansky Pudlak type 2 (HPS2) associated heterozygous compound mutation in AP3B1

DOI: 10.1016/j.scr.2021.102444

Authors: Cathelijn E.M. Aarts; Ellie Karampini; Tatjana Wüst; Steven Webbers; Eszter Varga; Judy Geissler; Jan Voorberg; Marieke von Lindern; Ruben Bierings; Emile van den Akker; Taco W. Kuijpers

Step 1: iPSC/ESC maintainance

day 0 ~ 2

Generation and characterization of cardiac valve endothelial-like cells from human pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

Generation and characterization of cardiac valve endothelial-like cells from human pluripotent stem cells

DOI: 10.1038/s42003-021-02571-7

Authors: LinXi Cheng; MingHui Xie; WeiHua Qiao; Yu Song; YanYong Zhang; YingChao Geng; WeiLin Xu; Lin Wang; Zheng Wang; Kai Huang; NianGuo Dong; YuHua Sun

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table.

Generation and characterization of three induced pluripotent stem cell lines for modeling coronary artery vasospasm

Mon, 01 Jan 0001 00:00:00 +0000

Generation and characterization of three induced pluripotent stem cell lines for modeling coronary artery vasospasm

DOI: 10.1016/j.scr.2024.103644

Authors: Ines Ross Tacco; Joseph Olshausen; Tse Yuan Chan; Naima Turbes; Ming-Yow Hung; Chi-Tai Yeh; Patricia K. Nguyen; Karim Sallam; Nazish Sayed; Ian Y. Chen

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “StemMACS iPS-Brew XF”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table.

Generation and validation of a PITX2–EGFP reporter line of human induced pluripotent stem cells enables isolation of periocular mesenchymal cells

Mon, 01 Jan 0001 00:00:00 +0000

Generation and validation of a PITX2–EGFP reporter line of human induced pluripotent stem cells enables isolation of periocular mesenchymal cells

DOI: 10.1074/jbc.RA119.010713

Authors: Toru Okubo; Ryuhei Hayashi; Shun Shibata; Yuji Kudo; Yuki Ishikawa; Saki Inoue; Yuki Kobayashi; Ai Honda; Yoichi Honma; Satoshi Kawasaki; Kohji Nishida

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “StemFit medium”. For ECM, use “Laminin” at “0.005” “g/m2”.

Generation of a doxycycline-inducible ETV2 expression cell line using PiggyBac transposase system

Mon, 01 Jan 0001 00:00:00 +0000

Generation of a doxycycline-inducible ETV2 expression cell line using PiggyBac transposase system

DOI: 10.1016/j.scr.2022.102985

Authors: Meng Zhou; Jie Ni; Pufeng Huang; Xujie Liu

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human ESC in “PGM1 medium” for “1” days. “ESC” should be “unspecified regulation” for “ETV2”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Generation of an EFNB2-2A-mCherry reporter human embryonic stem cell line using CRISPR/Cas9-mediated site-specific homologous recombination

Mon, 01 Jan 0001 00:00:00 +0000

Generation of an EFNB2-2A-mCherry reporter human embryonic stem cell line using CRISPR/Cas9-mediated site-specific homologous recombination

DOI: 10.1016/j.scr.2021.102241

Authors: Ying Huang; Hongchun Wu; Xinglong Han; Jie Wu; Miao Yu; Zhen-Ao Zhao; Zhenya Shen; Shijun Hu; Wei Lei

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human ESC in “E8”. “ESC” should be “unspecified regulation” for “EFNB2”. For ECM, use “Matrigel” at “N/A” “N/A”.

Generation of anti-GD2 CAR macrophages from human pluripotent stem cells for cancer immunotherapies

Mon, 01 Jan 0001 00:00:00 +0000

Generation of anti-GD2 CAR macrophages from human pluripotent stem cells for cancer immunotherapies

DOI: 10.1016/j.stemcr.2022.12.012

Authors: Jue Zhang; Sarah Webster; Bret Duffin; Matthew N. Bernstein; John Steill; Scott Swanson; Matthew H. Forsberg; Jennifer Bolin; Matthew E. Brown; Aditi Majumder; Christian M. Capitini; Ron Stewart; James A. Thomson; Igor I. Slukvin

Step 1: endothelial 1

day 0 ~ 6

culture “unspecified method” unknown species cell in “unspecified medium” for “6” days. Medium should be changed once in “6” days.

Generation of FOS gene knockout lines from a human embryonic stem cell line using CRISPR/Cas9

Mon, 01 Jan 0001 00:00:00 +0000

Generation of FOS gene knockout lines from a human embryonic stem cell line using CRISPR/Cas9

DOI: 10.1016/j.scr.2019.101479

Authors: Chunfu Li; Qing Wang; Zhiyong Peng; Yuchen Lin; Huaying Liu; Xiuling Yang; Shan Li; XiaoTing Liu; Jingru Chen

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human ESC in “mTeSR”. “ESC” should be “downregulated” for “FOS”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table.

Generation of fully functional hepatocyte-like organoids from human induced pluripotent stem cells mixed with Endothelial Cells

Mon, 01 Jan 0001 00:00:00 +0000

Generation of fully functional hepatocyte-like organoids from human induced pluripotent stem cells mixed with Endothelial Cells

DOI: 10.1038/s41598-019-45514-3

Authors: Giuseppe Pettinato; Sylvain Lehoux; Rajesh Ramanathan; Mohamed M. Salem; Li-Xia He; Oluwatoyosi Muse; Robert Flaumenhaft; Melissa T. Thompson; Emily A. Rouse; Richard D. Cummings; Xuejun Wen; Robert A. Fisher

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “Matrigel” at “N/A” “N/A”.

Generation of functional endothelial cells with progenitor-like features from murine induced pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

Generation of functional endothelial cells with progenitor-like features from murine induced pluripotent stem cells

DOI: 10.1016/j.vph.2016.07.008

Authors: Neli Kachamakova-Trojanowska; Witold Nowak; Krzysztof Szade; Jacek Stepniewski; Karolina Bukowska-Strakova; Monika Zukowska; Hevidar Taha; Antonina Chmura-Skirlinska; Michael Beilharz; Jozef Dulak; Alicja Jozkowicz

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” mouse PSC in “unspecified medium”.

Ideally, “PSC” should express “SSEA-1”, “NANOG”, “PECAM1”, “CD34”, “Tie-2”, “CD105” at the end of culture.

Generation of human induced pluripotent stem cell line EURACi015-A from a patient affected by dilated cardiomyopathy carrying the Lamin A/C p.Glu161Lys mutation

Mon, 01 Jan 0001 00:00:00 +0000

Generation of human induced pluripotent stem cell line EURACi015-A from a patient affected by dilated cardiomyopathy carrying the Lamin A/C p.Glu161Lys mutation

DOI: 10.1016/j.scr.2023.103172

Authors: Giada Cattelan; Laura Sophie Frommelt; Chiara Volani; Andrea Colliva; Giulio Ciucci; Alessia Paldino; Matteo Dal Ferro; Marina Di Segni; Rosamaria Silipigni; Peter P. Pramstaller; Marzia De Bortoli; Serena Zacchigna; Alessandra Rossini

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

Generation of human induced pluripotent stem cell-derived liver buds with chemically defined and animal origin-free media

Mon, 01 Jan 0001 00:00:00 +0000

Generation of human induced pluripotent stem cell-derived liver buds with chemically defined and animal origin-free media

DOI: 10.1038/s41598-020-73908-1

Authors: Keisuke Sekine; Shimpei Ogawa; Syusaku Tsuzuki; Tatsuya Kobayashi; Kazuki Ikeda; Noriko Nakanishi; Kenta Takeuchi; Eriko Kanai; Yugo Otake; Satoshi Okamoto; Tsuyoshi Kobayashi; Takanori Takebe; Hideki Taniguchi

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “StemFit AK03N”. For ECM, use “laminin 511E8” at “N/A” “N/A”.

Generation of human iPSC-derived 3D bile duct within liver organoid by incorporating human iPSC-derived blood vessel

Mon, 01 Jan 0001 00:00:00 +0000

Generation of human iPSC-derived 3D bile duct within liver organoid by incorporating human iPSC-derived blood vessel

DOI: 10.1038/s41467-024-51487-3

Authors: Erica Carolina; Yoshiki Kuse; Ayumu Okumura; Kenji Aoshima; Tomomi Tadokoro; Shinya Matsumoto; Eriko Kanai; Takashi Okumura; Toshiharu Kasai; Souichiro Yamabe; Yuji Nishikawa; Kiyoshi Yamaguchi; Yoichi Furukawa; Naoki Tanimizu; Hideki Taniguchi

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “StemFit AK02N”. For ECM, use “Laminin-511E8” at “N/A” “N/A”.

Generation of kidney organoids from human pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

Generation of kidney organoids from human pluripotent stem cells

DOI: 10.1038/nprot.2016.098

Authors: Minoru Takasato; Pei X Er; Han S Chiu; Melissa H Little

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “MEF-CM” for “1” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

	concentration	unit
FGF	1.0e-5	g/l
PSA	—	—
knockout serum replacement	—	—
NEAA	—	—
GlutaMAX	—	—
2-mercaptoethanol	—	—

Ideally, “PSC” should be obtained at the end of culture.

Generation of Lungs by Blastocyst Complementation in Apneumic Fgf10-Deficient Mice

Mon, 01 Jan 0001 00:00:00 +0000

Generation of Lungs by Blastocyst Complementation in Apneumic Fgf10-Deficient Mice

DOI: 10.1016/j.celrep.2020.107626

Authors: Akihiko Kitahara; Qingsong Ran; Kanako Oda; Akihiro Yasue; Manabu Abe; Xulu Ye; Toshikuni Sasaoka; Masanori Tsuchida; Kenji Sakimura; Yoichi Ajioka; Yasuo Saijo; Qiliang Zhou

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” mouse ESC in “Knockout DMEM”. Medium should contain factors shown in the following table.

	concentration	unit
FBS	8.8	%
KSR	8.8	%
NEAA	8.8e-5	mol/l
Glutamine	0.0018	mol/l
2-mercaptoethanol	8.8e-5	mol/l
LIF	884000	U/l

Ideally, “ESC” should express “GFP” at the end of culture.

Generation of multilineage liver organoids with luminal vasculature and bile ducts from human pluripotent stem cells via modulation of Notch signaling

Mon, 01 Jan 0001 00:00:00 +0000

Generation of multilineage liver organoids with luminal vasculature and bile ducts from human pluripotent stem cells via modulation of Notch signaling

DOI: 10.1186/s13287-023-03235-5

Authors: Hyo Jin Kim; Gyeongmin Kim; Kyun Yoo Chi; Hyemin Kim; Yu Jin Jang; Seongyea Jo; Jihun Lee; Youngseok Lee; Dong-Hun Woo; Choongseong Han; Sang Kyum Kim; Han-Jin Park; Jong-Hoon Kim

Step 1: Primitive streak 1

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” unknown species cell in “unspecified medium”.

Generation of Multipotential Mesendodermal Progenitors from Mouse Embryonic Stem Cells via Sustained Wnt Pathway Activation

Mon, 01 Jan 0001 00:00:00 +0000

Generation of Multipotential Mesendodermal Progenitors from Mouse Embryonic Stem Cells via Sustained Wnt Pathway Activation

DOI: 10.1074/jbc.M704287200

Authors: Manjiri Manohar Bakre; Aina Hoi; Jamie Chen Yee Mong; Yvonne Yiling Koh; Kee Yew Wong; Lawrence W. Stanton

Step 1: iPSC/ESC maintainance

day 0 ~ 2

culture “adherent” mouse ESC in “DMEM” for “2” days. For ECM, use “Gelatin” at “0.1” “%”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Generation of pure lymphatic endothelial cells from human pluripotent stem cells and their therapeutic effects on wound repair

Mon, 01 Jan 0001 00:00:00 +0000

Generation of pure lymphatic endothelial cells from human pluripotent stem cells and their therapeutic effects on wound repair

DOI: 10.1038/srep11019

Authors: Shin-Jeong Lee; Changwon Park; Ji Yoon Lee; Sangsung Kim; Pil Jae Kwon; Woansang Kim; Yong Heui Jeon; Eugine Lee; Young-sup Yoon

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “unspecified medium”.

Step 2: endothelial 1

day 1 ~ 8

Generation of vascular endothelial and smooth muscle cells from human pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

Generation of vascular endothelial and smooth muscle cells from human pluripotent stem cells

DOI: 10.1038/ncb3205

Authors: Christoph Patsch; Ludivine Challet-Meylan; Eva C. Thoma; Eduard Urich; Tobias Heckel; John F. O’Sullivan; Stephanie J. Grainger; Friedrich G. Kapp; Lin Sun; Klaus Christensen; Yulei Xia; Mary H. C. Florido; Wei He; Wei Pan; Michael Prummer; Curtis R. Warren; Roland Jakob-Roetne; Ulrich Certa; Ravi Jagasia; Per-Ola Freskgård; Isaac Adatto; Dorothee Kling; Paul Huang; Leonard I. Zon; Elliot L. Chaikof; Robert E. Gerszten; Martin Graf; Roberto Iacone; Chad A. Cowan

Generation of vascularized pancreatic progenitors through co-differentiation of endoderm and mesoderm from human pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

Generation of vascularized pancreatic progenitors through co-differentiation of endoderm and mesoderm from human pluripotent stem cells

DOI: 10.1186/s13287-024-04120-5

Authors: Xiaopu Sang; Junming Xu; Yihang Wang; Jingyi Li; Jiasen Xu; Xiaoni Chen; Xianjie Shi; Fenfang Wu

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “mTeSR” for “1” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should be changed once in “1” days.

Ideally, “PSC” should express “NANOG”, “POU5F1” at the end of culture.

Generation, expansion and functional analysis of endothelial cells and pericytes derived from human pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

Generation, expansion and functional analysis of endothelial cells and pericytes derived from human pluripotent stem cells

DOI: 10.1038/nprot.2014.102

Authors: Valeria V Orlova; Francijna E van den Hil; Sandra Petrus-Reurer; Yvette Drabsch; Peter ten Dijke; Christine L Mummery

Step 1: iPSC/ESC maintainance

day 0 ~ 3

culture “adherent” human PSC in “mTeSR” for “3” days. For ECM, use “Matrigel” at “0.2” “g/m2”. Medium should contain factors shown in the following table, and should be changed once in “3” days.

Generation, functional analysis and applications of isogenic three-dimensional self-aggregating cardiac microtissues from human pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

Generation, functional analysis and applications of isogenic three-dimensional self-aggregating cardiac microtissues from human pluripotent stem cells

DOI: 10.1038/s41596-021-00497-2

Authors: Giulia Campostrini; Viviana Meraviglia; Elisa Giacomelli; Ruben W. J. van Helden; Loukia Yiangou; Richard P. Davis; Milena Bellin; Valeria V. Orlova; Christine L. Mummery

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “E8” for “1” days. For ECM, use “Vitronectin” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Genomic chart guiding embryonic stem cell cardiopoiesis

Mon, 01 Jan 0001 00:00:00 +0000

Genomic chart guiding embryonic stem cell cardiopoiesis

DOI: 10.1186/gb-2008-9-1-r6

Authors: Randolph S Faustino; Atta Behfar; Carmen Perez-Terzic; Andre Terzic

Step 1: iPSC/ESC maintainance

day 0 ~ 2

culture “unspecified method” mouse ESC in “GMEM” for “2” days. Medium should contain factors shown in the following table, and should be changed once in “2” days.

	concentration	unit
FBS	7.5	%

Ideally, “ESC” should be obtained at the end of culture.

Glycan characteristics of human heart constituent cells maintaining organ function: relatively stable glycan profiles in cellular senescence

Mon, 01 Jan 0001 00:00:00 +0000

Glycan characteristics of human heart constituent cells maintaining organ function: relatively stable glycan profiles in cellular senescence

DOI: 10.1007/s10522-021-09940-z

Authors: Yoko Itakura; Norihiko Sasaki; Masashi Toyoda

No steps available

Graft immaturity and safety concerns in transplanted human kidney organoids

Mon, 01 Jan 0001 00:00:00 +0000

Graft immaturity and safety concerns in transplanted human kidney organoids

DOI: 10.1038/s12276-019-0336-x

Authors: Sun Ah Nam; Eunjeong Seo; Jin Won Kim; Hyung Wook Kim; Hong Lim Kim; Kyuryung Kim; Tae-Min Kim; Ji Hyeon Ju; Ivan G. Gomez; Kohei Uchimura; Benjamin D. Humphreys; Chul Woo Yang; Jae Yeon Lee; Jin Kim; Dong Woo Cho; Benjamin S. Freedman; Yong Kyun Kim

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

Grafted human-induced pluripotent stem cells-derived oligodendrocyte progenitor cells combined with human umbilical vein endothelial cells contribute to functional recovery following spinal cord injury

Mon, 01 Jan 0001 00:00:00 +0000

Grafted human-induced pluripotent stem cells-derived oligodendrocyte progenitor cells combined with human umbilical vein endothelial cells contribute to functional recovery following spinal cord injury

DOI: 10.1186/s13287-024-03651-1

Authors: Qian Li; Sumei Liu; Tianqi Zheng; Mo Li; Boling Qi; Liping Zhou; Bochao Liu; Dan Ma; Chao Zhao; Zhiguo Chen

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “E8” for “1” days. “PSC” should be “unspecified regulation” for “GFP”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Graphene Oxide promotes embryonic stem cell differentiation to haematopoietic lineage

Mon, 01 Jan 0001 00:00:00 +0000

Graphene Oxide promotes embryonic stem cell differentiation to haematopoietic lineage

DOI: 10.1038/srep25917

Authors: Eva Garcia-Alegria; Maria Iliut; Monika Stefanska; Claudio Silva; Sebastian Heeg; Susan J. Kimber; Valerie Kouskoff; Georges Lacaud; Aravind Vijayaraghavan; Kiran Batta

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human ESC in “E8”. For ECM, use “Geltrex” at “N/A” “N/A”.

Ideally, “ESC” should be obtained at the end of culture.

Growth-factor reduced Matrigel source influences stem cell derived brain microvascular endothelial cell barrier properties

Mon, 01 Jan 0001 00:00:00 +0000

Growth-factor reduced Matrigel source influences stem cell derived brain microvascular endothelial cell barrier properties

DOI: 10.1186/s12987-016-0030-5

Authors: Ronak Patel; Abraham J. Alahmad

Step 1: iPSC/ESC maintainance

day 0 ~ 5

culture “adherent” human PSC in “E8” for “5” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should be changed once in “5” days.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: endothelial 1

day 5 ~ 6

GSK-3 inhibition reverts mesenchymal transition in primary human corneal endothelial cells

Mon, 01 Jan 0001 00:00:00 +0000

GSK-3 inhibition reverts mesenchymal transition in primary human corneal endothelial cells

DOI: 10.1016/j.ejcb.2023.151302

Authors: Eleonora Maurizi; Alessia Merra; Claudio Macaluso; Davide Schiroli; Graziella Pellegrini

No steps available

GSK3β inhibition activates the CDX/HOX pathway and promotes hemogenic endothelial progenitor differentiation from human pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

GSK3β inhibition activates the CDX/HOX pathway and promotes hemogenic endothelial progenitor differentiation from human pluripotent stem cells

DOI: 10.1016/j.exphem.2015.09.007

Authors: Kenji Kitajima; Marino Nakajima; Mai Kanokoda; Michael Kyba; Abhijit Dandapat; Jakub Tolar; Megumu K. Saito; Masashi Toyoda; Akihiro Umezawa; Takahiko Hara

Step 1: iPSC/ESC maintainance

day 0 ~ 1

Guiding T lymphopoiesis from pluripotent stem cells by defined transcription factors

Mon, 01 Jan 0001 00:00:00 +0000

Guiding T lymphopoiesis from pluripotent stem cells by defined transcription factors

DOI: 10.1038/s41422-019-0251-7

Authors: Rongqun Guo; Fangxiao Hu; Qitong Weng; Cui Lv; Hongling Wu; Lijuan Liu; Zongcheng Li; Yang Zeng; Zhijie Bai; Mengyun Zhang; Yuting Liu; Xiaofei Liu; Chengxiang Xia; Tongjie Wang; Peiqing Zhou; Kaitao Wang; Yong Dong; Yuxuan Luo; Xiangzhong Zhang; Yuxian Guan; Yang Geng; Juan Du; Yangqiu Li; Yu Lan; Jiekai Chen; Bing Liu; Jinyong Wang

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

Haematopoietic stem and progenitor cell heterogeneity is inherited from the embryonic endothelium

Mon, 01 Jan 0001 00:00:00 +0000

Haematopoietic stem and progenitor cell heterogeneity is inherited from the embryonic endothelium

DOI: 10.1038/s41556-023-01187-9

Authors: Joey J. Ghersi; Gabriel Baldissera; Jared Hintzen; Stephanie A. Luff; Siyuan Cheng; Ivan Fan Xia; Christopher M. Sturgeon; Stefania Nicoli

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human ESC in “unspecified medium” for “1” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should be changed once in “1” days.

Step 2: Primitive streak 1

day 1 ~ 4

Hepatic patch by stacking patient-specific liver progenitor cell sheets formed on multiscale electrospun fibers promotes regenerative therapy for liver injury

Mon, 01 Jan 0001 00:00:00 +0000

Hepatic patch by stacking patient-specific liver progenitor cell sheets formed on multiscale electrospun fibers promotes regenerative therapy for liver injury

DOI: 10.1016/j.biomaterials.2021.120899

Authors: Yohan Kim; Young Won Kim; Seung Bum Lee; Kyojin Kang; Sangtae Yoon; Dongho Choi; Suk-Hee Park; Jaemin Jeong

No steps available

High-efficient serum-free differentiation of endothelial cells from human iPS cells

Mon, 01 Jan 0001 00:00:00 +0000

High-efficient serum-free differentiation of endothelial cells from human iPS cells

DOI: 10.1186/s13287-022-02924-x

Authors: Sarkawt Hamad; Daniel Derichsweiler; John Antonydas Gaspar; Konrad Brockmeier; Jürgen Hescheler; Agapios Sachinidis; Kurt Paul Pfannkuche

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “E8” for “1” days. For ECM, use “Matrigel” at “0.1” “g/m2”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

	concentration	unit
L-ascorbic acid phosphate magnesium	0.064	g/l
insulin	0.02	g/l
Transferrin	0.005	g/l
sodium selenite	1.4e-5	g/l
Heparin	1.0e-4	g/l
FGF	1.0e-4	g/l
TGFb	2.0e-6	g/l
Y27632	5.0e-6	mol/l

Ideally, “PSC” should be obtained at the end of culture.

Hox genes are involved in vascular wall-resident multipotent stem cell differentiation into smooth muscle cells

Mon, 01 Jan 0001 00:00:00 +0000

Hox genes are involved in vascular wall-resident multipotent stem cell differentiation into smooth muscle cells

DOI: 10.1038/srep02178

Authors: Diana Klein; Mohamed Benchellal; Veronika Kleff; Heinz Günther Jakob; Süleyman Ergün

No steps available

Human definitive haemogenic endothelium and arterial vascular endothelium represent distinct lineages

Mon, 01 Jan 0001 00:00:00 +0000

Human definitive haemogenic endothelium and arterial vascular endothelium represent distinct lineages

DOI: 10.1038/ncb3161

Authors: Andrea Ditadi; Christopher M. Sturgeon; Joanna Tober; Geneve Awong; Marion Kennedy; Amanda D. Yzaguirre; Lisa Azzola; Elizabeth S. Ng; Edouard G. Stanley; Deborah L. French; Xin Cheng; Paul Gadue; Nancy A. Speck; Andrew G. Elefanty; Gordon Keller

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human ESC in “unspecified medium” for “1” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should be changed once in “1” days.

Human dental pulp pluripotent-like stem cells promote wound healing and muscle regeneration

Mon, 01 Jan 0001 00:00:00 +0000

Human dental pulp pluripotent-like stem cells promote wound healing and muscle regeneration

DOI: 10.1186/s13287-017-0621-3

Authors: Ester Martínez-Sarrà; Sheyla Montori; Carlos Gil-Recio; Raquel Núñez-Toldrà; Domiziana Costamagna; Alessio Rotini; Maher Atari; Aernout Luttun; Maurilio Sampaolesi

Step 1: iPSC/ESC maintainance

day 0 ~ 4

culture “adherent” human DPPSC in “DMEM-low glucose” for “4” days. For ECM, use “FN1” at “1.0e-4” “g/l”. Medium should contain factors shown in the following table, and should be changed once in “4” days.

Human embryonic stem cell-derived CD34+ cells function as MSC progenitor cells

Mon, 01 Jan 0001 00:00:00 +0000

Human embryonic stem cell-derived CD34+ cells function as MSC progenitor cells

DOI: 10.1016/j.bone.2010.06.020

Authors: Ross A. Kopher; Vesselin R. Penchev; Mohammad S. Islam; Katherine L. Hill; Sundeep Khosla; Dan S. Kaufman

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human ESC in “DMEM/F12”. Medium should contain factors shown in the following table.

	concentration	unit
KSR	15	%
NEAA	1	%
PSA	0.5	%
GlutaMAX	0.002	mol/l
2-mercaptoethanol	1.0e-4	mol/l
FGF	4.0e-6	g/l

Ideally, “ESC” should be obtained at the end of culture.

Human forebrain endothelial cell therapy for psychiatric disorders

Mon, 01 Jan 0001 00:00:00 +0000

Human forebrain endothelial cell therapy for psychiatric disorders

DOI: 10.1038/s41380-020-0839-9

Authors: Debkanya Datta; Sivan Subburaju; Sarah Kaye; Jugajyoti Baruah; Yong Kee Choi; Yeqi Nian; Jahan S. Khalili; Sangmi Chung; Abdallah Elkhal; Anju Vasudevan

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human ESC in “E8”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table.

	concentration	unit
EDTA	5.0e-4	mol/l

Step 2: mesoderm 1

day 1 ~ 3

Human iPS cell-derived mural cells as an in vitro model of hereditary cerebral small vessel disease

Mon, 01 Jan 0001 00:00:00 +0000

Human iPS cell-derived mural cells as an in vitro model of hereditary cerebral small vessel disease

DOI: 10.1186/s13041-020-00573-w

Authors: Yumi Yamamoto; Katsutoshi Kojima; Daisuke Taura; Masakatsu Sone; Kazuo Washida; Naohiro Egawa; Takayuki Kondo; Eiko N. Minakawa; Kayoko Tsukita; Takako Enami; Hidekazu Tomimoto; Toshiki Mizuno; Raj N. Kalaria; Nobuya Inagaki; Ryosuke Takahashi; Mariko Harada-Shiba; Masafumi Ihara; Haruhisa Inoue

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

Human iPS cell-engineered cardiac tissue sheets with cardiomyocytes and vascular cells for cardiac regeneration

Mon, 01 Jan 0001 00:00:00 +0000

Human iPS cell-engineered cardiac tissue sheets with cardiomyocytes and vascular cells for cardiac regeneration

DOI: 10.1038/srep06716

Authors: Hidetoshi Masumoto; Takeshi Ikuno; Masafumi Takeda; Hiroyuki Fukushima; Akira Marui; Shiori Katayama; Tatsuya Shimizu; Tadashi Ikeda; Teruo Okano; Ryuzo Sakata; Jun K. Yamashita

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “MEF-CM”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table.

Human isogenic cells of the neurovascular unit exert transcriptomic cell type-specific effects on a blood-brain barrier in vitro model of late-onset Alzheimer disease

Mon, 01 Jan 0001 00:00:00 +0000

Human isogenic cells of the neurovascular unit exert transcriptomic cell type-specific effects on a blood-brain barrier in vitro model of late-onset Alzheimer disease

DOI: 10.1186/s12987-023-00471-y

Authors: Undine Haferkamp; Carla Hartmann; Chaudhry Luqman Abid; Andreas Brachner; Alevtina Höchner; Anna Gerhartl; Bernadette Harwardt; Selin Leckzik; Jennifer Leu; Marco Metzger; Marina Nastainczyk-Wulf; Winfried Neuhaus; Sabrina Oerter; Ole Pless; Dan Rujescu; Matthias Jung; Antje Appelt-Menzel

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “mTeSR” for “1” days. For ECM, use “Matrigel” at “8.3e-5” “g/well”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Human liver organoids generated with single donor-derived multiple cells rescue mice from acute liver failure

Mon, 01 Jan 0001 00:00:00 +0000

Human liver organoids generated with single donor-derived multiple cells rescue mice from acute liver failure

DOI: 10.1186/s13287-017-0749-1

Authors: Yun-Zhong Nie; Yun-Wen Zheng; Miyuki Ogawa; Etsuko Miyagi; Hideki Taniguchi

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “Matrigel” at “N/A” “N/A”.

Ideally, “PSC” should express “POU5F1”, “NANOG”, “SOX2”, “LIN28A”, “KLF4”, “SSEA4”, “TRA-1-60” at the end of culture.

Human Organ-Specific Endothelial Cell Heterogeneity

Mon, 01 Jan 0001 00:00:00 +0000

Human Organ-Specific Endothelial Cell Heterogeneity

DOI: 10.1016/j.isci.2018.05.003

Authors: Raluca Marcu; Yoon Jung Choi; Jun Xue; Chelsea L. Fortin; Yuliang Wang; Ryan J. Nagao; Jin Xu; James W. MacDonald; Theo K. Bammler; Charles E. Murry; Kimberly Muczynski; Kelly R. Stevens; Jonathan Himmelfarb; Stephen M. Schwartz; Ying Zheng

No steps available

Human pancreatic microenvironment promotes β-cell differentiation via non-canonical WNT5A/JNK and BMP signaling

Mon, 01 Jan 0001 00:00:00 +0000

Human pancreatic microenvironment promotes β-cell differentiation via non-canonical WNT5A/JNK and BMP signaling

DOI: 10.1038/s41467-022-29646-1

Authors: Jolanta Chmielowiec; Wojciech J. Szlachcic; Diane Yang; Marissa A. Scavuzzo; Katrina Wamble; Alejandro Sarrion-Perdigones; Omaima M. Sabek; Koen J. T. Venken; Malgorzata Borowiak

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human ESC in “E8” for “1” days. For ECM, use “Geltrex” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Human umbilical cord mesenchymal stem cells improve the reserve function of perimenopausal ovary via a paracrine mechanism

Mon, 01 Jan 0001 00:00:00 +0000

Human umbilical cord mesenchymal stem cells improve the reserve function of perimenopausal ovary via a paracrine mechanism

DOI: 10.1186/s13287-017-0514-5

Authors: Jia Li; QiuXian Mao; JingJun He; HaoQing She; Zhi Zhang; ChunYan Yin

No steps available

Human Vascular Tissue Models Formed from Human Induced Pluripotent Stem Cell Derived Endothelial Cells

Mon, 01 Jan 0001 00:00:00 +0000

Human Vascular Tissue Models Formed from Human Induced Pluripotent Stem Cell Derived Endothelial Cells

DOI: 10.1007/s12015-014-9549-5

Authors: David G. Belair; Jordan A. Whisler; Jorge Valdez; Jeremy Velazquez; James A. Molenda; Vernella Vickerman; Rachel Lewis; Christine Daigh; Tyler D. Hansen; David A. Mann; James A. Thomson; Linda G. Griffith; Roger D. Kamm; Michael P. Schwartz; William L. Murphy

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

Human vascularized macrophage-islet organoids to model immune-mediated pancreatic β cell pyroptosis upon viral infection

Mon, 01 Jan 0001 00:00:00 +0000

Human vascularized macrophage-islet organoids to model immune-mediated pancreatic β cell pyroptosis upon viral infection

DOI: 10.1016/j.stem.2024.08.007

Authors: Liuliu Yang; Yuling Han; Tuo Zhang; Xue Dong; Jian Ge; Aadita Roy; Jiajun Zhu; Tiankun Lu; J. Jeya Vandana; Neranjan de Silva; Catherine C. Robertson; Jenny Z. Xiang; Chendong Pan; Yanjie Sun; Jianwen Que; Todd Evans; Chengyang Liu; Wei Wang; Ali Naji; Stephen C.J. Parker; Robert E. Schwartz; Shuibing Chen

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

Hypoxia drives hematopoiesis with the enhancement of T lineage through eliciting arterial specification of hematopoietic endothelial progenitors from hESC

Mon, 01 Jan 0001 00:00:00 +0000

Hypoxia drives hematopoiesis with the enhancement of T lineage through eliciting arterial specification of hematopoietic endothelial progenitors from hESC

DOI: 10.1186/s13287-022-02967-0

Authors: Ning Wang; Chuxin Chen; Yang Cheng; Yingjie Fu; Zhiyong Zhong; Yu Yang; Ling Lv; Honglin Chen; Jian Huang; Yuyou Duan

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human ESC in “DMEM/F12”. For ECM, use “Fibroblast” at “N/A” “N/A”. Medium should contain factors shown in the following table.

Hypoxia-enhanced Blood-Brain Barrier Chip recapitulates human barrier function and shuttling of drugs and antibodies

Mon, 01 Jan 0001 00:00:00 +0000

Hypoxia-enhanced Blood-Brain Barrier Chip recapitulates human barrier function and shuttling of drugs and antibodies

DOI: 10.1038/s41467-019-10588-0

Authors: Tae-Eun Park; Nur Mustafaoglu; Anna Herland; Ryan Hasselkus; Robert Mannix; Edward A. FitzGerald; Rachelle Prantil-Baun; Alexander Watters; Olivier Henry; Maximilian Benz; Henry Sanchez; Heather J. McCrea; Liliana Christova Goumnerova; Hannah W. Song; Sean P. Palecek; Eric Shusta; Donald E. Ingber

Step 1: iPSC/ESC maintainance

day 0 ~ 3

culture “adherent” human PSC in “mTeSR” for “3” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should be changed once in “3” days.

ICU patient-on-a-chip emulating orchestration of mast cells and cerebral organoids in neuroinflammation

Mon, 01 Jan 0001 00:00:00 +0000

ICU patient-on-a-chip emulating orchestration of mast cells and cerebral organoids in neuroinflammation

DOI: 10.1038/s42003-024-07313-z

Authors: Pelin Saglam-Metiner; Sena Yanasik; Yusuf Caglar Odabasi; Jennifer Modamio; Moritz Negwer; Cigir Biray-Avci; Ayse Guler; Ali Erturk; Ender Yildirim; Ozlem Yesil-Celiktas

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table.

ID3 contributes to the acquisition of molecular stem cell-like signature in microvascular endothelial cells: Its implication for understanding microvascular diseases

Mon, 01 Jan 0001 00:00:00 +0000

ID3 contributes to the acquisition of molecular stem cell-like signature in microvascular endothelial cells: Its implication for understanding microvascular diseases

DOI: 10.1016/j.mvr.2015.01.006

Authors: Jayanta K. Das; Norbert F. Voelkel; Quentin Felty

Step 1: endothelial 1

day 0 ~ 4

culture “spheroid” human EC in “DMEM/F12” for “4” days. Medium should contain factors shown in the following table, and should be changed once in “4” days.

	concentration	unit
B27	—	—
SU5416	—	—

Ideally, “EC” should be obtained at the end of culture.

Identification of a combination of transcription factors that synergistically increases endothelial cell barrier resistance

Mon, 01 Jan 0001 00:00:00 +0000

Identification of a combination of transcription factors that synergistically increases endothelial cell barrier resistance

DOI: 10.1038/s41598-020-60688-x

Authors: Filip Roudnicky; Bo Kyoung Kim; Yanjun Lan; Roland Schmucki; Verena Küppers; Klaus Christensen; Martin Graf; Christoph Patsch; Mark Burcin; Claas Aiko Meyer; Peter D. Westenskow; Chad A. Cowan

Step 1: endothelial 1

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” unknown species cell in “StemPro”. Medium should contain factors shown in the following table.

Identification of a retinoic acid-dependent haemogenic endothelial progenitor from human pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

Identification of a retinoic acid-dependent haemogenic endothelial progenitor from human pluripotent stem cells

DOI: 10.1038/s41556-022-00898-9

Authors: Stephanie A. Luff; J. Philip Creamer; Sara Valsoni; Carissa Dege; Rebecca Scarfò; Analisa Dacunto; Sara Cascione; Lauren N. Randolph; Eleonora Cavalca; Ivan Merelli; Samantha A. Morris; Andrea Ditadi; Christopher M. Sturgeon

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “unspecified medium” for “1” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should be changed once in “1” days.

Identification of Cell Fate Determining Transcription Factors for Generating Brain Endothelial Cells

Mon, 01 Jan 0001 00:00:00 +0000

Identification of Cell Fate Determining Transcription Factors for Generating Brain Endothelial Cells

DOI: 10.1007/s12015-025-10842-7

Authors: Roya Ramezankhani; Jonathan De Smedt; Burak Toprakhisar; Bernard K. van der Veer; Tine Tricot; Gert Vanmarcke; Bradley Balaton; Leo van Grunsven; Massoud Vosough; Yoke Chin Chai; Catherine Verfaillie

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human ESC in “mTeSR” for “1” days. “ESC” should be “upregulated” for “ETV2”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Identification of novel genes and networks governing hematopoietic stem cell development

Mon, 01 Jan 0001 00:00:00 +0000

Identification of novel genes and networks governing hematopoietic stem cell development

DOI: 10.15252/embr.201642395

Authors: Tianxu Han; Chao‐Shun Yang; Kung‐Yen Chang; Danhua Zhang; Farhad B Imam; Tariq M Rana

Step 1: iPSC/ESC maintainance

day 0 ~ 2

culture “adherent” mouse ESC in “DMEM” for “2” days. Medium should contain factors shown in the following table, and should be changed once in “2” days.

	concentration	unit
FBS	15	%
NEAA	1.0e-4	mol/l
glutamine	0.002	mol/l
PSA	0.05	g/l
streptomycin	0.075	g/l
MTG	8.6e-4	%
LIF	1.0e+6	U/l

Ideally, “ESC” should be obtained at the end of culture.

Identification of the Hemogenic Endothelial Progenitor and Its Direct Precursor in Human Pluripotent Stem Cell Differentiation Cultures

Mon, 01 Jan 0001 00:00:00 +0000

Identification of the Hemogenic Endothelial Progenitor and Its Direct Precursor in Human Pluripotent Stem Cell Differentiation Cultures

DOI: 10.1016/j.celrep.2012.08.002

Authors: Kyung-Dal Choi; Maxim A. Vodyanik; Padma Priya Togarrati; Kran Suknuntha; Akhilesh Kumar; Fnu Samarjeet; Mitchell D. Probasco; Shulan Tian; Ron Stewart; James A. Thomson; Igor I. Slukvin

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “unspecified medium”.

Step 2: mesoderm 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

Immortalized CNS pericytes are quiescent smooth muscle actin-negative and pluripotent

Mon, 01 Jan 0001 00:00:00 +0000

Immortalized CNS pericytes are quiescent smooth muscle actin-negative and pluripotent

DOI: 10.1016/j.mvr.2011.04.003

Authors: Paula Dore-Duffy; Afroza Mehedi; Xueqian Wang; Michael Bradley; Richard Trotter; Alexander Gow

No steps available

Improving hematopoietic differentiation from human induced pluripotent stem cells by the modulation of Hippo signaling with a diarylheptanoid derivative

Mon, 01 Jan 0001 00:00:00 +0000

Improving hematopoietic differentiation from human induced pluripotent stem cells by the modulation of Hippo signaling with a diarylheptanoid derivative

DOI: 10.1186/s13287-024-03686-4

Authors: Umnuaychoke Thongsa-ad; Anongnat Wongpan; Wasinee Wongkummool; Phaewa Chaiwijit; Kwanchanok Uppakara; Gorawin Chaiyakitpattana; Passanan Singpant; Pirut Tong-ngam; Amnat Chukhan; Wachirachai Pabuprappap; Sirapope Wongniam; Apichart Suksamrarn; Suradej Hongeng; Usanarat Anurathapan; Kasem Kulkeaw; Alisa Tubsuwan; Kanit Bhukhai

Step 1: iPSC/ESC maintainance

day 0 ~ 1

In vitro conversion of adult murine endothelial cells to hematopoietic stem cells

Mon, 01 Jan 0001 00:00:00 +0000

In vitro conversion of adult murine endothelial cells to hematopoietic stem cells

DOI: 10.1038/s41596-018-0060-3

Authors: José Gabriel Barcia Durán; Raphaël Lis; Tyler M. Lu; Shahin Rafii

Step 1: endothelial 1

day 0 ~ 2

culture “adherent” unknown species cell and mouse EC and human EC in “M199” for “2” days. For ECM, use “FN1” at “1” “g/l”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

In Vitro Differentiation of Gata2 and Ly6a Reporter Embryonic Stem Cells Corresponds to In Vivo Waves of Hematopoietic Cell Generation

Mon, 01 Jan 0001 00:00:00 +0000

In Vitro Differentiation of Gata2 and Ly6a Reporter Embryonic Stem Cells Corresponds to In Vivo Waves of Hematopoietic Cell Generation

DOI: 10.1016/j.stemcr.2017.11.018

Authors: Mari-Liis Kauts; Carmen Rodriguez-Seoane; Polynikis Kaimakis; Sandra C. Mendes; Xabier Cortés-Lavaud; Undine Hill; Elaine Dzierzak

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” mouse ESC in “DMEM”. Medium should contain factors shown in the following table.

	concentration	unit
FBS	15	%
GlutaMAX	0.002	mol/l
Na-pyruvate	0.001	mol/l
PSA	1	%
2-mercaptoethanol	5.0e-5	mol/l
NEAA	1.0e-4	mol/l
LIF	1.0e+6	U/l

Ideally, “ESC” should be obtained at the end of culture.

In vitro modelling of alveolar repair at the air-liquid interface using alveolar epithelial cells derived from human induced pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

In vitro modelling of alveolar repair at the air-liquid interface using alveolar epithelial cells derived from human induced pluripotent stem cells

DOI: 10.1038/s41598-020-62226-1

Authors: Sander van Riet; Dennis K. Ninaber; Harald M. M. Mikkers; Teresa D. Tetley; Carolina R. Jost; Aat A. Mulder; Thijs Pasman; Danielle Baptista; André A. Poot; Roman Truckenmüller; Christine L. Mummery; Christian Freund; Robbert J. Rottier; Pieter S. Hiemstra

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

In Vitro System for Differentiating Pluripotent Neural Crest Cells into Smooth Muscle Cells

Mon, 01 Jan 0001 00:00:00 +0000

In Vitro System for Differentiating Pluripotent Neural Crest Cells into Smooth Muscle Cells

DOI: 10.1074/jbc.273.11.5993

Authors: Mukesh K. Jain; Matthew D. Layne; Masafumi Watanabe; Michael T. Chin; Mark W. Feinberg; Nicholas E.S. Sibinga; Chung-Ming Hsieh; Shaw-Fang Yet; Derek L. Stemple; Mu-En Lee

No steps available

Induced pluripotent stem cell-derived cells model brain microvascular endothelial cell glucose metabolism

Mon, 01 Jan 0001 00:00:00 +0000

Induced pluripotent stem cell-derived cells model brain microvascular endothelial cell glucose metabolism

DOI: 10.1186/s12987-022-00395-z

Authors: Callie M. Weber; Bilal Moiz; Sophia M. Zic; Viviana Alpízar Vargas; Andrew Li; Alisa Morss Clyne

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR-Plus”. For ECM, use “Matrigel” at “N/A” “N/A”.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: endothelial 1

day 1 ~ 2

Induced pluripotent stem cell-derived endothelial progenitor cells attenuate ischemic acute kidney injury and cardiac dysfunction

Mon, 01 Jan 0001 00:00:00 +0000

Induced pluripotent stem cell-derived endothelial progenitor cells attenuate ischemic acute kidney injury and cardiac dysfunction

DOI: 10.1186/s13287-018-1092-x

Authors: Wen-Ching Shen; Yu-Hsiang Chou; Hsiang-Po Huang; Jenn-Feng Sheen; Shih-Chieh Hung; Hsin-Fu Chen

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “unspecified medium”. “PSC” should be “upregulated” for “GFP”. For ECM, use “Fibroblast” at “N/A” “N/A”.

Ideally, “PSC” should express “CD133” at the end of culture.

Induced pluripotent stem cells as a model for diabetes investigation

Mon, 01 Jan 0001 00:00:00 +0000

Induced pluripotent stem cells as a model for diabetes investigation

DOI: 10.1038/srep08597

Authors: J. Stepniewski; N. Kachamakova-Trojanowska; D. Ogrocki; M. Szopa; M. Matlok; M. Beilharz; G. Dyduch; M. T. Malecki; A. Jozkowicz; J. Dulak

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” unknown species cell and mouse PSC in “DMEM” for “1” days. For ECM, use “MEF feeder” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Induced Pluripotent Stem Cells to Model Human Fibrodysplasia Ossificans Progressiva

Mon, 01 Jan 0001 00:00:00 +0000

Induced Pluripotent Stem Cells to Model Human Fibrodysplasia Ossificans Progressiva

DOI: 10.1016/j.stemcr.2015.10.020

Authors: Jie Cai; Valeria V. Orlova; Xiujuan Cai; Elisabeth M.W. Eekhoff; Keqin Zhang; Duanqing Pei; Guangjin Pan; Christine L. Mummery; Peter ten Dijke

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” human PSC in “mTeSR”.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: Primitive streak 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

Induced pluripotent stem cells with NOTCH1 gene mutation show impaired differentiation into smooth muscle and endothelial cells: Implications for bicuspid aortic valve-related aortopathy

Mon, 01 Jan 0001 00:00:00 +0000

DOI: 10.1016/j.jtcvs.2018.02.087

Authors: Jiao Jiao; Weihua Tian; Ping Qiu; Elizabeth L. Norton; Michael M. Wang; Y. Eugene Chen; Bo Yang

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “E8”. “PSC” should be “downregulated” for “NOTCH1”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table.

Inflammatory Responses and Barrier Function of Endothelial Cells Derived from Human Induced Pluripotent Stem Cells

Mon, 01 Jan 0001 00:00:00 +0000

Inflammatory Responses and Barrier Function of Endothelial Cells Derived from Human Induced Pluripotent Stem Cells

DOI: 10.1016/j.stemcr.2018.03.012

Authors: Oleh V. Halaidych; Christian Freund; Francijna van den Hil; Daniela C.F. Salvatori; Mara Riminucci; Christine L. Mummery; Valeria V. Orlova

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “unspecified medium”.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: endothelial 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

Inhibition of angiogenesis by the secretome from iPSC-derived retinal ganglion cells with Leber's hereditary optic neuropathy-like phenotypes

Mon, 01 Jan 0001 00:00:00 +0000

Inhibition of angiogenesis by the secretome from iPSC-derived retinal ganglion cells with Leber’s hereditary optic neuropathy-like phenotypes

DOI: 10.1016/j.biopha.2024.117270

Authors: Shih-Yuan Peng; Chih-Ying Chen; Hsin Chen; Yi-Ping Yang; Mong-Lien Wang; Fu-Ting Tsai; Chian-Shiu Chien; Pei-Yu Weng; En-Tung Tsai; I-Chieh Wang; Chih-Chien Hsu; Tai-Chi Lin; De-Kuang Hwang; Shih-Jen Chen; Shih-Hwa Chiou; Chuan-Chin Chiao; Yueh Chien

Step 1: iPSC/ESC maintainance

day 0 ~ 5

culture “adherent” human PSC in “StemFlex medium” for “5” days. For ECM, use “Geltrex” at “N/A” “N/A”. Medium should be changed once in “5” days.

Inhibition of the E3 ligase UBR5 stabilizes TERT and protects vascular organoids from oxidative stress

Mon, 01 Jan 0001 00:00:00 +0000

Inhibition of the E3 ligase UBR5 stabilizes TERT and protects vascular organoids from oxidative stress

DOI: 10.1186/s12967-024-05887-0

Authors: Haijing Zhao; Nian Cao; Qi Liu; Yingyue Zhang; Rui Jin; Huiying Lai; Li Zheng; Honghong Zhang; Yue Zhu; Yuhan Ma; Zengao Yang; Zhengfeng Wu; Weini Li; Yuqi Liu; Long Cheng; Yundai Chen

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” human PSC and human PSC in “E8”. “PSC” should be “downregulated” for “UBR5”. “PSC” should be “upregulated” for “TERT”.

Inhibition of transforming growth factor beta signaling pathway promotes differentiation of human induced pluripotent stem cell-derived brain microvascular endothelial-like cells

Mon, 01 Jan 0001 00:00:00 +0000

Inhibition of transforming growth factor beta signaling pathway promotes differentiation of human induced pluripotent stem cell-derived brain microvascular endothelial-like cells

DOI: 10.1186/s12987-020-00197-1

Authors: Misaki Yamashita; Hiromasa Aoki; Tadahiro Hashita; Takahiro Iwao; Tamihide Matsunaga

Step 1: iPSC/ESC maintainance

day 0 ~ 3

culture “adherent” human PSC in “StemSure hPSC medium” for “3” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “3” days.

Integrated Multiparametric High-Content Profiling of Endothelial Cells

Mon, 01 Jan 0001 00:00:00 +0000

Integrated Multiparametric High-Content Profiling of Endothelial Cells

DOI: 10.1177/2472555218820848

Authors: Erika Wiseman; Annj Zamuner; Zuming Tang; James Rogers; Sabrina Munir; Lucy Di Silvio; Davide Danovi; Lorenzo Veschini

No steps available

Integration-free reprogramming of human umbilical arterial endothelial cells into induced pluripotent stem cells IHSTMi001-A

Mon, 01 Jan 0001 00:00:00 +0000

Integration-free reprogramming of human umbilical arterial endothelial cells into induced pluripotent stem cells IHSTMi001-A

DOI: 10.1016/j.scr.2018.08.007

Authors: Huilin Li; Haiyun Pei; Jiqin Nie; Mingyi Qu; Zeng Fan; Yali Jia; Lijuan He; Xue Nan; Wen Yue; Xuetao Pei

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “E8”. For ECM, use “Geltrex” at “N/A” “N/A”.

Ideally, “PSC” should express “POU5F1”, “SOX2”, “NANOG”, “SSEA-4”, “TRA-1-60” at the end of culture.

Integrative epigenomic and transcriptomic analysis reveals the requirement of JUNB for hematopoietic fate induction

Mon, 01 Jan 0001 00:00:00 +0000

Integrative epigenomic and transcriptomic analysis reveals the requirement of JUNB for hematopoietic fate induction

DOI: 10.1038/s41467-022-30789-4

Authors: Xia Chen; Peiliang Wang; Hui Qiu; Yonglin Zhu; Xingwu Zhang; Yaxuan Zhang; Fuyu Duan; Shuangyuan Ding; Jianying Guo; Yue Huang; Jie Na

Step 1: iPSC/ESC maintainance

day 0 ~ 1

Intrinsic epigenetic control of angiogenesis in induced pluripotent stem cell-derived endothelium regulates vascular regeneration

Mon, 01 Jan 0001 00:00:00 +0000

Intrinsic epigenetic control of angiogenesis in induced pluripotent stem cell-derived endothelium regulates vascular regeneration

DOI: 10.1038/s41536-022-00223-w

Authors: Bria L. Macklin; Ying-Yu Lin; Kevin Emmerich; Emily Wisniewski; Brian M. Polster; Konstantinos Konstantopoulos; Jeff S. Mumm; Sharon Gerecht

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “E8” for “1” days. For ECM, use “Vitronectin” at “N/A” “N/A”. Medium should be changed once in “1” days.

Ideally, “PSC” should be obtained at the end of culture.

Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells

DOI: 10.1038/nbt.2239

Authors: Micha Drukker; Chad Tang; Reza Ardehali; Yuval Rinkevich; Jun Seita; Andrew S Lee; Adriane R Mosley; Irving L Weissman; Yoav Soen

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “Matrigel” at “N/A” “N/A”.

Step 2: Primitive streak 1

day 1 ~ 4

KSCBi005-A-10(hiPSC-HIF1αKO), a HIF1α knockout human induced pluripotent stem cell line, for demonstrating the role of cellular response to hypoxia

Mon, 01 Jan 0001 00:00:00 +0000

KSCBi005-A-10(hiPSC-HIF1αKO), a HIF1α knockout human induced pluripotent stem cell line, for demonstrating the role of cellular response to hypoxia

DOI: 10.1016/j.scr.2024.103415

Authors: Hyeong-jun Han; Jung-Hyun Kim

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “E8”. “PSC” should be “downregulated” for “HIF-1α”. For ECM, use “vitronectin” at “N/A” “N/A”.

Ideally, “PSC” should express “POU5F1”, “SSEA4”, “TRA-1-60”, “TRA-1-81” at the end of culture.

Lamin A/C-dependent chromatin architecture safeguards naïve pluripotency to prevent aberrant cardiovascular cell fate and function

Mon, 01 Jan 0001 00:00:00 +0000

Lamin A/C-dependent chromatin architecture safeguards naïve pluripotency to prevent aberrant cardiovascular cell fate and function

DOI: 10.1038/s41467-022-34366-7

Authors: Yinuo Wang; Adel Elsherbiny; Linda Kessler; Julio Cordero; Haojie Shi; Heike Serke; Olga Lityagina; Felix A. Trogisch; Mona Malek Mohammadi; Ibrahim El-Battrawy; Johannes Backs; Thomas Wieland; Joerg Heineke; Gergana Dobreva

Step 1: iPSC/ESC maintainance

day 0 ~ 2

culture “adherent” mouse ESC in “DMEM/F12” for “2” days. For ECM, use “Gelatin” at “0.1” “%”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Laminin 221 fragment is suitable for the differentiation of human induced pluripotent stem cells into brain microvascular endothelial-like cells with robust barrier integrity

Mon, 01 Jan 0001 00:00:00 +0000

Laminin 221 fragment is suitable for the differentiation of human induced pluripotent stem cells into brain microvascular endothelial-like cells with robust barrier integrity

DOI: 10.1186/s12987-020-00186-4

Authors: Hiromasa Aoki; Misaki Yamashita; Tadahiro Hashita; Takahiro Iwao; Tamihide Matsunaga

Step 1: iPSC/ESC maintainance

day 0 ~ 3

culture “adherent” human PSC in “StemSure hPSC medium” for “3” days. For ECM, use “LN221F” at “0.01” “g/m2”. Medium should contain factors shown in the following table, and should be changed once in “3” days.

Laminin 411 mediates endothelial specification via multiple signaling axes that converge on β-catenin

Mon, 01 Jan 0001 00:00:00 +0000

Laminin 411 mediates endothelial specification via multiple signaling axes that converge on β-catenin

DOI: 10.1016/j.stemcr.2022.01.005

Authors: Mikayla L. Hall; Sophie Givens; Natasha Santosh; Michelina Iacovino; Michael Kyba; Brenda M. Ogle

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “Matrigel” at “N/A” “N/A”.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: Primitive streak 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells

DOI: 10.1038/srep35680

Authors: Ryo Ohta; Akira Niwa; Yukimasa Taniguchi; Naoya M. Suzuki; Junko Toga; Emiko Yagi; Norikazu Saiki; Yoko Nishinaka-Arai; Chihiro Okada; Akira Watanabe; Tatsutoshi Nakahata; Kiyotoshi Sekiguchi; Megumu K. Saito

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “LM511-E8” at “0.004” “g/m2”.

Step 2: mesoderm 1

day 1 ~ 3

Lineage specification of Flk-1+ progenitors is associated with divergent Sox7 expression in cardiopoiesis

Mon, 01 Jan 0001 00:00:00 +0000

Lineage specification of Flk-1+ progenitors is associated with divergent Sox7 expression in cardiopoiesis

DOI: 10.1016/j.diff.2008.10.012

Authors: Timothy J. Nelson; Anca Chiriac; Randolph S. Faustino; Ruben J. Crespo-Diaz; Atta Behfar; Andre Terzic

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” mouse ESC in “GMEM”. Medium should contain factors shown in the following table.

	concentration	unit
pyruvate	—	—
NEAA	—	—
2-mercaptoethanol	—	—
FCS	7.5	%
LIF	—	—

Step 2: Primitive streak 1

day 1 ~ 3

Liver specification of human iPSC-derived endothelial cells transplanted into mouse liver

Mon, 01 Jan 0001 00:00:00 +0000

Liver specification of human iPSC-derived endothelial cells transplanted into mouse liver

DOI: 10.1016/j.jhepr.2024.101023

Authors: Kiryu K. Yap; Jan Schröder; Yi-Wen Gerrand; Aleksandar Dobric; Anne M. Kong; Adrian M. Fox; Brett Knowles; Simon W. Banting; Andrew G. Elefanty; Eduoard G. Stanley; George C. Yeoh; Glen P. Lockwood; Victoria C. Cogger; Wayne A. Morrison; Jose M. Polo; Geraldine M. Mitchell

Step 1: iPSC/ESC maintainance

day 0 ~ 1

Long-term engrafting multilineage hematopoietic cells differentiated from human induced pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

Long-term engrafting multilineage hematopoietic cells differentiated from human induced pluripotent stem cells

DOI: 10.1038/s41587-024-02360-7

Authors: Elizabeth S. Ng; Gulcan Sarila; Jacky Y. Li; Hasindu S. Edirisinghe; Ritika Saxena; Shicheng Sun; Freya F. Bruveris; Tanya Labonne; Nerida Sleebs; Alexander Maytum; Raymond Y. Yow; Chantelle Inguanti; Ali Motazedian; Vincenzo Calvanese; Sandra Capellera-Garcia; Feiyang Ma; Hieu T. Nim; Mirana Ramialison; Constanze Bonifer; Hanna K. A. Mikkola; Edouard G. Stanley; Andrew G. Elefanty

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

Long-term self-renewing human epicardial cells generated from pluripotent stem cells under defined xeno-free conditions

Mon, 01 Jan 0001 00:00:00 +0000

Long-term self-renewing human epicardial cells generated from pluripotent stem cells under defined xeno-free conditions

DOI: 10.1038/s41551-016-0003

Authors: Xiaoping Bao; Xiaojun Lian; Timothy A. Hacker; Eric G. Schmuck; Tongcheng Qian; Vijesh J. Bhute; Tianxiao Han; Mengxuan Shi; Lauren Drowley; Alleyn T. Plowright; Qing-Dong Wang; Marie-Jose Goumans; Sean P. Palecek

Step 1: iPSC/ESC maintainance

day 0 ~ 1

Manipulation of a VEGF-Notch signaling circuit drives formation of functional vascular endothelial progenitors from human pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

Manipulation of a VEGF-Notch signaling circuit drives formation of functional vascular endothelial progenitors from human pluripotent stem cells

DOI: 10.1038/cr.2014.59

Authors: Makoto Sahara; Emil M Hansson; Oliver Wernet; Kathy O Lui; Daniela Später; Kenneth R Chien

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “mTeSR-1” for “1” days. For ECM, use “Matrigel” at “0.3” “g/l”. Medium should be changed once in “1” days.

Ideally, “PSC” should be obtained at the end of culture.

Mapping human pluripotent stem cell differentiation pathways using high throughput single-cell RNA-sequencing

Mon, 01 Jan 0001 00:00:00 +0000

Mapping human pluripotent stem cell differentiation pathways using high throughput single-cell RNA-sequencing

DOI: 10.1186/s13059-018-1426-0

Authors: Xiaoping Han; Haide Chen; Daosheng Huang; Huidong Chen; Lijiang Fei; Chen Cheng; He Huang; Guo-Cheng Yuan; Guoji Guo

Step 1: iPSC/ESC maintainance

day 0 ~ 20

culture “unspecified method” human ESC in “RSeT medium” for “20” days. Medium should contain factors shown in the following table, and should be changed once in “20” days.

	concentration	unit
PD0325901	—	—

Ideally, “ESC” should express “POU5F1”, “NANOG”, “PRDM14”, “NODAL”, “LEFTY2”, “GDF3”, “KLF4”, “DNMT3L”, “IL6ST”, “DPPA2”, “TDGF1”, “SOX2” at the end of culture.

Mature human induced pluripotent stem cell-derived cardiomyocytes promote angiogenesis through alpha-B crystallin

Mon, 01 Jan 0001 00:00:00 +0000

Mature human induced pluripotent stem cell-derived cardiomyocytes promote angiogenesis through alpha-B crystallin

DOI: 10.1186/s13287-023-03468-4

Authors: Yuki Tanaka; Shin Kadota; Jian Zhao; Hideki Kobayashi; Satomi Okano; Masaki Izumi; Yusuke Honda; Hajime Ichimura; Naoko Shiba; Takeshi Uemura; Yuko Wada; Shinichiro Chuma; Tsutomu Nakada; Shugo Tohyama; Keiichi Fukuda; Mitsuhiko Yamada; Tatsuichiro Seto; Koichiro Kuwahara; Yuji Shiba

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “E8”. For ECM, use “Matrigel” at “N/A” “N/A”.

Mature induced-pluripotent-stem-cell-derived human podocytes reconstitute kidney glomerular-capillary-wall function on a chip

Mon, 01 Jan 0001 00:00:00 +0000

Mature induced-pluripotent-stem-cell-derived human podocytes reconstitute kidney glomerular-capillary-wall function on a chip

DOI: 10.1038/s41551-017-0069

Authors: Samira Musah; Akiko Mammoto; Thomas C. Ferrante; Sauveur S. F. Jeanty; Mariko Hirano-Kobayashi; Tadanori Mammoto; Kristen Roberts; Seyoon Chung; Richard Novak; Miles Ingram; Tohid Fatanat-Didar; Sandeep Koshy; James C. Weaver; George M. Church; Donald E. Ingber

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “mTeSR” for “1” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should be changed once in “1” days.

Medial HOXA genes demarcate haematopoietic stem cell fate during human development

Mon, 01 Jan 0001 00:00:00 +0000

Medial HOXA genes demarcate haematopoietic stem cell fate during human development

DOI: 10.1038/ncb3354

Authors: Diana R. Dou; Vincenzo Calvanese; Maria I. Sierra; Andrew T. Nguyen; Arazin Minasian; Pamela Saarikoski; Rajkumar Sasidharan; Christina M. Ramirez; Jerome A. Zack; Gay M. Crooks; Zoran Galic; Hanna K. A. Mikkola

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human ESC in “DMEM/F12”. For ECM, use “MEF feeder” at “N/A” “N/A”. Medium should contain factors shown in the following table.

MEIS1 Regulates Hemogenic Endothelial Generation, Megakaryopoiesis, and Thrombopoiesis in Human Pluripotent Stem Cells by Targeting TAL1 and FLI1

Mon, 01 Jan 0001 00:00:00 +0000

MEIS1 Regulates Hemogenic Endothelial Generation, Megakaryopoiesis, and Thrombopoiesis in Human Pluripotent Stem Cells by Targeting TAL1 and FLI1

DOI: 10.1016/j.stemcr.2017.12.017

Authors: Hongtao Wang; Cuicui Liu; Xin Liu; Mengge Wang; Dan Wu; Jie Gao; Pei Su; Tatsutoshi Nakahata; Wen Zhou; Yuanfu Xu; Lihong Shi; Feng Ma; Jiaxi Zhou

Step 1: iPSC/ESC maintainance

day 0 ~ 1

Mesp1 Marked Cardiac Progenitor Cells Repair Infarcted Mouse Hearts

Mon, 01 Jan 0001 00:00:00 +0000

Mesp1 Marked Cardiac Progenitor Cells Repair Infarcted Mouse Hearts

DOI: 10.1038/srep31457

Authors: Yu Liu; Li Chen; Andrea Diaz Diaz; Ashley Benham; Xueping Xu; Cori S. Wijaya; Faisal Fa’ak; Weijia Luo; Benjamin Soibam; Alon Azares; Wei Yu; Qiongying Lyu; M. David Stewart; Preethi Gunaratne; Austin Cooney; Bradley K. McConnell; Robert J. Schwartz

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” mouse ESC in “DMEM”. Medium should contain factors shown in the following table.

Microgravity simulation activates Cdc42 via Rap1GDS1 to promote vascular branch morphogenesis during vasculogenesis

Mon, 01 Jan 0001 00:00:00 +0000

Microgravity simulation activates Cdc42 via Rap1GDS1 to promote vascular branch morphogenesis during vasculogenesis

DOI: 10.1016/j.scr.2017.11.002

Authors: Shouli Wang; Zhao Yin; Bei Zhao; Yanmei Qi; Jie Liu; Saum A. Rahimi; Leonard Y. Lee; Shaohua Li

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” mouse ESC in “unspecified medium”.

Step 2: mesoderm 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

Migratory and anti-fibrotic programmes define the regenerative potential of human cardiac progenitors

Mon, 01 Jan 0001 00:00:00 +0000

Migratory and anti-fibrotic programmes define the regenerative potential of human cardiac progenitors

DOI: 10.1038/s41556-022-00899-8

Authors: Christine M. Poch; Kylie S. Foo; Maria Teresa De Angelis; Karin Jennbacken; Gianluca Santamaria; Andrea Bähr; Qing-Dong Wang; Franziska Reiter; Nadja Hornaschewitz; Dorota Zawada; Tarik Bozoglu; Ilaria My; Anna Meier; Tatjana Dorn; Simon Hege; Miia L. Lehtinen; Yat Long Tsoi; Daniel Hovdal; Johan Hyllner; Sascha Schwarz; Stefanie Sudhop; Victoria Jurisch; Marcella Sini; Mick D. Fellows; Matthew Cummings; Jonathan Clarke; Ricardo Baptista; Elif Eroglu; Eckhard Wolf; Nikolai Klymiuk; Kun Lu; Roland Tomasi; Andreas Dendorfer; Marco Gaspari; Elvira Parrotta; Giovanni Cuda; Markus Krane; Daniel Sinnecker; Petra Hoppmann; Christian Kupatt; Regina Fritsche-Danielson; Alessandra Moretti; Kenneth R. Chien; Karl-Ludwig Laugwitz

Mitochondrial permeability transition dictates mitochondrial maturation upon switch in cellular identity of hematopoietic precursors

Mon, 01 Jan 0001 00:00:00 +0000

Mitochondrial permeability transition dictates mitochondrial maturation upon switch in cellular identity of hematopoietic precursors

DOI: 10.1038/s42003-024-06671-y

Authors: Sandeep P. Dumbali; Paulina D. Horton; Travis I. Moore; Pamela L. Wenzel

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” mouse PSC in “DMEM”. For ECM, use “Gelatin” at “N/A” “N/A”. Medium should contain factors shown in the following table.

	concentration	unit
PSA	—	—
streptomycin	—	—
FBS	—	—
NEAA	—	—
LIF	—	—

Ideally, “PSC” should be obtained at the end of culture.

Mitophagy mediates metabolic reprogramming of induced pluripotent stem cells undergoing endothelial differentiation

Mon, 01 Jan 0001 00:00:00 +0000

Mitophagy mediates metabolic reprogramming of induced pluripotent stem cells undergoing endothelial differentiation

DOI: 10.1016/j.jbc.2021.101410

Authors: Sarah Krantz; Young-Mee Kim; Shubhi Srivastava; Joseph W. Leasure; Peter T. Toth; Glenn Marsboom; Jalees Rehman

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table.

	concentration	unit
Dispase	1000	U/l
Y27632	5.0e-6	mol/l

Step 2: mesoderm 1

day 1 ~ 2

Modeling and rescue of defective blood–brain barrier function of induced brain microvascular endothelial cells from childhood cerebral adrenoleukodystrophy patients

Mon, 01 Jan 0001 00:00:00 +0000

Modeling and rescue of defective blood–brain barrier function of induced brain microvascular endothelial cells from childhood cerebral adrenoleukodystrophy patients

DOI: 10.1186/s12987-018-0094-5

Authors: Catherine A. A. Lee; Hannah S. Seo; Anibal G. Armien; Frank S. Bates; Jakub Tolar; Samira M. Azarin

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “E8”. For ECM, use “Matrigel” at “N/A” “N/A”.

Ideally, “PSC” should be obtained at the end of culture.

Modeling Congenital Adrenal Hyperplasia and Testing Interventions for Adrenal Insufficiency Using Donor-Specific Reprogrammed Cells

Mon, 01 Jan 0001 00:00:00 +0000

Modeling Congenital Adrenal Hyperplasia and Testing Interventions for Adrenal Insufficiency Using Donor-Specific Reprogrammed Cells

DOI: 10.1016/j.celrep.2018.01.003

Authors: Gerard Ruiz-Babot; Mariya Balyura; Irene Hadjidemetriou; Sharon J. Ajodha; David R. Taylor; Lea Ghataore; Norman F. Taylor; Undine Schubert; Christian G. Ziegler; Helen L. Storr; Maralyn R. Druce; Evelien F. Gevers; William M. Drake; Umasuthan Srirangalingam; Gerard S. Conway; Peter J. King; Louise A. Metherell; Stefan R. Bornstein; Leonardo Guasti

No steps available

Molecular profiling of the basement membrane of pluripotent epiblast cells in post-implantation stage mouse embryos

Mon, 01 Jan 0001 00:00:00 +0000

Molecular profiling of the basement membrane of pluripotent epiblast cells in post-implantation stage mouse embryos

DOI: 10.1016/j.reth.2019.04.010

Authors: Sugiko Futaki; Itsuko Nakano; Miwa Kawasaki; Noriko Sanzen; Kiyotoshi Sekiguchi

No steps available

Motor neurons and endothelial cells additively promote development and fusion of human iPSC-derived skeletal myocytes

Mon, 01 Jan 0001 00:00:00 +0000

Motor neurons and endothelial cells additively promote development and fusion of human iPSC-derived skeletal myocytes

DOI: 10.1186/s13395-024-00336-4

Authors: Suradip Das; Melanie C. Hilman; Feikun Yang; Foteini Mourkioti; Wenli Yang; D. Kacy Cullen

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “StemMACS iPS-Brew medium” for “1” days. Medium should be changed once in “1” days.

Ideally, “PSC” should be obtained at the end of culture.

Mouse embryonic stem cell-derived blood–brain barrier model: applicability to studying antibody triggered receptor mediated transcytosis

Mon, 01 Jan 0001 00:00:00 +0000

Mouse embryonic stem cell-derived blood–brain barrier model: applicability to studying antibody triggered receptor mediated transcytosis

DOI: 10.1186/s12987-023-00437-0

Authors: Anna Jezierski; Jez Huang; Arsalan S. Haqqani; Julie Haukenfrers; Ziying Liu; Ewa Baumann; Caroline Sodja; Claudie Charlebois; Christie E. Delaney; Alexandra T. Star; Qing Liu; Danica B. Stanimirovic

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”.

Step 2: Primitive streak 1

day 1 ~ 6

MSX2 suppression through inhibition of TGFβ signaling enhances hematopoietic differentiation of human embryonic stem cells

Mon, 01 Jan 0001 00:00:00 +0000

MSX2 suppression through inhibition of TGFβ signaling enhances hematopoietic differentiation of human embryonic stem cells

DOI: 10.1186/s13287-020-01653-3

Authors: Hongtao Wang; Mengge Wang; Yu Wang; Yuqi Wen; Xiaoyuan Chen; Dan Wu; Pei Su; Wen Zhou; Lihong Shi; Jiaxi Zhou

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human ESC in “mTeSR”. For ECM, use “Matrigel” at “N/A” “N/A”.

Ideally, “ESC” should express “NANOG”, “SOX2”, “POU5F1” at the end of culture.

Multilineage differentiation potential of hematoendothelial progenitors derived from human induced pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

Multilineage differentiation potential of hematoendothelial progenitors derived from human induced pluripotent stem cells

DOI: 10.1186/s13287-020-01997-w

Authors: Ratchapong Netsrithong; Siriwal Suwanpitak; Bootsakorn Boonkaew; Kongtana Trakarnsanga; Lung-Ji Chang; Chartsiam Tipgomut; Chinnavuth Vatanashevanopakorn; Kovit Pattanapanyasat; Methichit Wattanapanitch

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “E8”. For ECM, use “Matrigel” at “2.5” “%”. Medium should contain factors shown in the following table.

Nod1-dependent NF-kB activation initiates hematopoietic stem cell specification in response to small Rho GTPases

Mon, 01 Jan 0001 00:00:00 +0000

Nod1-dependent NF-kB activation initiates hematopoietic stem cell specification in response to small Rho GTPases

DOI: 10.1038/s41467-023-43349-1

Authors: Xiaoyi Cheng; Radwa Barakat; Giulia Pavani; Masuma Khatun Usha; Rodolfo Calderon; Elizabeth Snella; Abigail Gorden; Yudi Zhang; Paul Gadue; Deborah L. French; Karin S. Dorman; Antonella Fidanza; Clyde A. Campbell; Raquel Espin-Palazon

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “StemPro hESC SFM” for “1” days. For ECM, use “Vitronectin” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Non-viral vector based gene transfection with human induced pluripotent stem cells derived cardiomyocytes

Mon, 01 Jan 0001 00:00:00 +0000

Non-viral vector based gene transfection with human induced pluripotent stem cells derived cardiomyocytes

DOI: 10.1038/s41598-019-50980-w

Authors: Shihua Tan; Zhonghao Tao; Szejie Loo; Liping Su; Xin Chen; Lei Ye

Step 1: iPSC/ESC maintainance

day 0 ~ 4

culture “adherent” human PSC in “mTeSR” for “4” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “4” days.

	concentration	unit
E8	50	%

Ideally, “PSC” should be obtained at the end of culture.

NOTCH signaling specifies arterial-type definitive hemogenic endothelium from human pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

NOTCH signaling specifies arterial-type definitive hemogenic endothelium from human pluripotent stem cells

DOI: 10.1038/s41467-018-04134-7

Authors: Gene I. Uenishi; Ho Sun Jung; Akhilesh Kumar; Mi Ae Park; Brandon K. Hadland; Ethan McLeod; Matthew Raymond; Oleg Moskvin; Catherine E. Zimmerman; Derek J. Theisen; Scott Swanson; Owen J. Tamplin; Leonard I. Zon; James A. Thomson; Irwin D. Bernstein; Igor I. Slukvin

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “E8” for “1” days. For ECM, use “Collagen” at “0.005” “g/m2”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Novel codon-optimized mini-intronic plasmid for efficient, inexpensive and xeno-free induction of pluripotency

Mon, 01 Jan 0001 00:00:00 +0000

Novel codon-optimized mini-intronic plasmid for efficient, inexpensive and xeno-free induction of pluripotency

DOI: 10.1038/srep08081

Authors: Sebastian Diecke; Jiamiao Lu; Jaecheol Lee; Vittavat Termglinchan; Nigel G. Kooreman; Paul W. Burridge; Antje D. Ebert; Jared M. Churko; Arun Sharma; Mark A. Kay; Joseph C. Wu

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “E8”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table.

Nox2 contributes to the arterial endothelial specification of mouse induced pluripotent stem cells by upregulating Notch signaling

Mon, 01 Jan 0001 00:00:00 +0000

Nox2 contributes to the arterial endothelial specification of mouse induced pluripotent stem cells by upregulating Notch signaling

DOI: 10.1038/srep33737

Authors: Xueling Kang; Xiangxiang Wei; Xinhong Wang; Li Jiang; Cong Niu; Jianyi Zhang; Sifeng Chen; Dan Meng

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” mouse PSC in “DMEM”. “PSC” should be “downregulated” for “Nox2”. Medium should contain factors shown in the following table.

Nox4 promotes endothelial differentiation through chromatin remodeling

Mon, 01 Jan 0001 00:00:00 +0000

Nox4 promotes endothelial differentiation through chromatin remodeling

DOI: 10.1016/j.redox.2022.102381

Authors: F. Hahner; F. Moll; T. Warwick; D.M. Hebchen; G.K. Buchmann; J. Epah; W. Abplanalp; T. Schader; S. Günther; R. Gilsbach; R.P. Brandes; K. Schröder

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” mouse PSC in “DMEM”. “PSC” should be “downregulated” for “Nox4”. For ECM, use “feeder cells” at “N/A” “N/A”. Medium should contain factors shown in the following table.

On-chip real-time impedance monitoring of hiPSC-derived and artificial basement membrane-supported endothelium

Mon, 01 Jan 0001 00:00:00 +0000

On-chip real-time impedance monitoring of hiPSC-derived and artificial basement membrane-supported endothelium

DOI: 10.1016/j.bios.2023.115324

Authors: Xiaochen Huang; Feng Liang; Boxin Huang; Haoyue Luo; Jian Shi; Li Wang; Juan Peng; Yong Chen

Step 1: iPSC/ESC maintainance

day 0 ~ 2

culture “adherent” human PSC in “E8” for “2” days. For ECM, use “vitronectin” at “1” “%”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Optimization and Critical Evaluation of Decellularization Strategies to Develop Renal Extracellular Matrix Scaffolds as Biological Templates for Organ Engineering and Transplantation

Mon, 01 Jan 0001 00:00:00 +0000

Optimization and Critical Evaluation of Decellularization Strategies to Develop Renal Extracellular Matrix Scaffolds as Biological Templates for Organ Engineering and Transplantation

DOI: 10.1111/ajt.12999

Authors: M. Caralt; J.S. Uzarski; S. Iacob; K.P. Obergfell; N. Berg; B.M. Bijonowski; K.M. Kiefer; H.H. Ward; A. Wandinger-Ness; W.M. Miller; Z.J. Zhang; M.M. Abecassis; J.A. Wertheim

No steps available

Optimization of Synthetic mRNA for Highly Efficient Translation and its Application in the Generation of Endothelial and Hematopoietic Cells from Human and Primate Pluripotent Stem Cells

Mon, 01 Jan 0001 00:00:00 +0000

Optimization of Synthetic mRNA for Highly Efficient Translation and its Application in the Generation of Endothelial and Hematopoietic Cells from Human and Primate Pluripotent Stem Cells

DOI: 10.1007/s12015-018-9805-1

Authors: Kran Suknuntha; Lihong Tao; Vera Brok-Volchanskaya; Saritha S. D’Souza; Akhilesh Kumar; Igor Slukvin

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “E8”. For ECM, use “vitronectin” at “N/A” “N/A”.

Ideally, “PSC” should be obtained at the end of culture.

Optimizing mesoderm progenitor selection and three-dimensional microniche culture allows highly efficient endothelial differentiation and ischemic tissue repair from human pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

Optimizing mesoderm progenitor selection and three-dimensional microniche culture allows highly efficient endothelial differentiation and ischemic tissue repair from human pluripotent stem cells

DOI: 10.1186/s13287-016-0455-4

Authors: Fengzhi Zhang; Lin Wang; Yaqian Li; Wei Liu; Fuyu Duan; Rujin Huang; Xi Chen; Sophia Chia-Ning Chang; Yanan Du; Jie Na

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “E8”. For ECM, use “Gelatin” at “0.1” “%”.

Orthogonally induced differentiation of stem cells for the programmatic patterning of vascularized organoids and bioprinted tissues

Mon, 01 Jan 0001 00:00:00 +0000

Orthogonally induced differentiation of stem cells for the programmatic patterning of vascularized organoids and bioprinted tissues

DOI: 10.1038/s41551-022-00856-8

Authors: Mark A. Skylar-Scott; Jeremy Y. Huang; Aric Lu; Alex H. M. Ng; Tomoya Duenki; Songlei Liu; Lucy L. Nam; Sarita Damaraju; George M. Church; Jennifer A. Lewis

Step 1: iPSC/ESC maintainance

day 0 ~ 1

Overexpression of GATA2 Enhances Development and Maintenance of Human Embryonic Stem Cell-Derived Hematopoietic Stem Cell-like Progenitors

Mon, 01 Jan 0001 00:00:00 +0000

Overexpression of GATA2 Enhances Development and Maintenance of Human Embryonic Stem Cell-Derived Hematopoietic Stem Cell-like Progenitors

DOI: 10.1016/j.stemcr.2019.05.007

Authors: Ya Zhou; Yonggang Zhang; Bo Chen; Yong Dong; Yimeng Zhang; Bin Mao; Xu Pan; Mowen Lai; Yijin Chen; Guohui Bian; Qiongxiu Zhou; Tatsutoshi Nakahata; Jiaxi Zhou; Min Wu; Feng Ma

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human ESC in “mTeSR”. For ECM, use “Matrigel” at “N/A” “N/A”.

P450 oxidoreductase regulates barrier maturation by mediating retinoic acid metabolism in a model of the human BBB

Mon, 01 Jan 0001 00:00:00 +0000

P450 oxidoreductase regulates barrier maturation by mediating retinoic acid metabolism in a model of the human BBB

DOI: 10.1016/j.stemcr.2022.07.010

Authors: Dor Zlotnik; Tatiana Rabinski; Aviv Halfon; Shira Anzi; Inbar Plaschkes; Hadar Benyamini; Yuval Nevo; Orly Yahalom Gershoni; Benyamin Rosental; Eli Hershkovitz; Ayal Ben-Zvi; Gad D. Vatine

Step 1: iPSC/ESC maintainance

day 0 ~ 3

culture “unspecified method” human PSC in “NutriStem” for “3” days. Medium should be changed once in “3” days.

Patient-derived organoids for precision oncology: a platform to facilitate clinical decision making

Mon, 01 Jan 0001 00:00:00 +0000

Patient-derived organoids for precision oncology: a platform to facilitate clinical decision making

DOI: 10.1186/s12885-023-11078-9

Authors: Swati Chitrangi; Pooja Vaity; Aishwarya Jamdar; Shweta Bhatt

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “Matrigel” at “N/A” “N/A”.

Ideally, “PSC” should express “POU5F1”, “NANOG”, “SOX2”, “SSEA4”, “TRA-1-81”, “TRA-1-60”, “ALP” at the end of culture.

Step 2: mesoderm 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

Patient-Induced Pluripotent Stem Cell–Derived Hepatostellate Organoids Establish a Basis for Liver Pathologies in Telomeropathies

Mon, 01 Jan 0001 00:00:00 +0000

Patient-Induced Pluripotent Stem Cell–Derived Hepatostellate Organoids Establish a Basis for Liver Pathologies in Telomeropathies

DOI: 10.1016/j.jcmgh.2023.06.003

Authors: Young-Jun Choi; Melissa S. Kim; Joshua H. Rhoades; Nicolette M. Johnson; Corbett T. Berry; Sarah Root; Qijun Chen; Yuhua Tian; Rafael J. Fernandez; Zvi Cramer; Stephanie Adams-Tzivelekidis; Ning Li; F. Brad Johnson; Christopher J. Lengner

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “StemMACS iPSC-Brew XF medium” for “1” days. “PSC” should be “unspecified regulation” for “DKC1”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Pigment Epithelium-Derived Factor Secreted from Retinal Pigment Epithelium Facilitates Apoptotic Cell Death of iPSC

Mon, 01 Jan 0001 00:00:00 +0000

Pigment Epithelium-Derived Factor Secreted from Retinal Pigment Epithelium Facilitates Apoptotic Cell Death of iPSC

DOI: 10.1038/srep02334

Authors: Hoshimi Kanemura; Masahiro J. Go; Naoki Nishishita; Noriko Sakai; Hiroyuki Kamao; Yoji Sato; Masayo Takahashi; Shin Kawamata

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “ReproFF2”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table.

Pluripotent stem cell model of Shwachman–Diamond syndrome reveals apoptotic predisposition of hemoangiogenic progenitors

Mon, 01 Jan 0001 00:00:00 +0000

Pluripotent stem cell model of Shwachman–Diamond syndrome reveals apoptotic predisposition of hemoangiogenic progenitors

DOI: 10.1038/s41598-020-71844-8

Authors: Takayuki Hamabata; Katsutsugu Umeda; Kagehiro Kouzuki; Takayuki Tanaka; Tomoo Daifu; Seishiro Nodomi; Satoshi Saida; Itaru Kato; Shiro Baba; Hidefumi Hiramatsu; Mitsujiro Osawa; Akira Niwa; Megumu K. Saito; Yasuhiko Kamikubo; Souichi Adachi; Yoshiko Hashii; Akira Shimada; Hiroyoshi Watanabe; Kenji Osafune; Keisuke Okita; Tatsutoshi Nakahata; Kenichiro Watanabe; Junko Takita; Toshio Heike

Step 1: iPSC/ESC maintainance

day 0 ~ 7

Pluripotent stem cell-induced skeletal muscle progenitor cells with givinostat promote myoangiogenesis and restore dystrophin in injured Duchenne dystrophic muscle

Mon, 01 Jan 0001 00:00:00 +0000

Pluripotent stem cell-induced skeletal muscle progenitor cells with givinostat promote myoangiogenesis and restore dystrophin in injured Duchenne dystrophic muscle

DOI: 10.1186/s13287-021-02174-3

Authors: Wanling Xuan; Mahmood Khan; Muhammad Ashraf

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “mTeSR” for “1” days. For ECM, use “vitronectin” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

	concentration	unit
Y27632	5.0e-6	mol/l

Step 2: mesoderm 1

day 1 ~ 3

Porcine ovarian cortex-derived putative stem cells can differentiate into endothelial cells in vitro

Mon, 01 Jan 0001 00:00:00 +0000

Porcine ovarian cortex-derived putative stem cells can differentiate into endothelial cells in vitro

DOI: 10.1007/s00418-021-02016-6

Authors: Kamil Wartalski; Gabriela Gorczyca; Jerzy Wiater; Zbigniew Tabarowski; Małgorzata Duda

Step 1: iPSC/ESC maintainance

day 0 ~ 2

culture “adherent” unknown species cell and pig PSC in “DMEM/F12” for “2” days. For ECM, use “poly-L-lysine” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Possible linkages between the inner and outer cellular states of human induced pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

Possible linkages between the inner and outer cellular states of human induced pluripotent stem cells

DOI: 10.1186/1752-0509-5-S1-S17

Authors: Shigeru Saito; Yasuko Onuma; Yuzuru Ito; Hiroaki Tateno; Masashi Toyoda; Akutsu Hidenori; Koichiro Nishino; Emi Chikazawa; Yoshihiro Fukawatase; Yoshitaka Miyagawa; Hajime Okita; Nobutaka Kiyokawa; Yohichi Shimma; Akihiro Umezawa; Jun Hirabayashi; Katsuhisa Horimoto; Makoto Asashima

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “iPSellon medium”. Medium should contain factors shown in the following table.

PRC2 specifies ectoderm lineages and maintains pluripotency in primed but not naïve ESCs

Mon, 01 Jan 0001 00:00:00 +0000

PRC2 specifies ectoderm lineages and maintains pluripotency in primed but not naïve ESCs

DOI: 10.1038/s41467-017-00668-4

Authors: Yongli Shan; Zechuan Liang; Qi Xing; Tian Zhang; Bo Wang; Shulan Tian; Wenhao Huang; Yanqi Zhang; Jiao Yao; Yanling Zhu; Ke Huang; Yujian Liu; Xiaoshan Wang; Qianyu Chen; Jian Zhang; Bizhi Shang; Shengbiao Li; Xi Shi; Baojian Liao; Cong Zhang; Keyu Lai; Xiaofen Zhong; Xiaodong Shu; Jinyong Wang; Hongjie Yao; Jiekai Chen; Duanqing Pei; Guangjin Pan

Preparation of iPS cell-derived CD31+ endothelial cells using three-dimensional suspension culture

Mon, 01 Jan 0001 00:00:00 +0000

Preparation of iPS cell-derived CD31+ endothelial cells using three-dimensional suspension culture

DOI: 10.1016/j.reth.2018.06.004

Authors: Shinako Masuda; Katsuhisa Matsuura; Tatsuya Shimizu

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “Primate ES Cell Medium”. Medium should contain factors shown in the following table.

	concentration	unit
FGF	5.0e-6	g/l

Ideally, “PSC” should be obtained at the end of culture.

Step 2: Primitive streak 1

day 1 ~ 3

Presence of a mutation in PSEN1 or PSEN2 gene is associated with an impaired brain endothelial cell phenotype in vitro

Mon, 01 Jan 0001 00:00:00 +0000

Presence of a mutation in PSEN1 or PSEN2 gene is associated with an impaired brain endothelial cell phenotype in vitro

DOI: 10.1186/s12987-020-00235-y

Authors: Snehal Raut; Ronak Patel; Abraham J. Al-Ahmad

Step 1: iPSC/ESC maintainance

day 0 ~ 5

culture “adherent” unknown species cell and human PSC in “E8” for “5” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should be changed once in “5” days.

Ideally, “PSC” should be obtained at the end of culture.

Protocol for differentiating hematopoietic progenitor cells from human pluripotent stem cells in chemically defined monolayer culture

Mon, 01 Jan 0001 00:00:00 +0000

Protocol for differentiating hematopoietic progenitor cells from human pluripotent stem cells in chemically defined monolayer culture

DOI: 10.1016/j.xpro.2024.103545

Authors: Shaokang Mo; Kengyuan Qu; Jun Shen; Kuangyu Yen

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human cell in “E8” for “1” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

	concentration	unit
Y27632	1.0e-5	mol/l

Ideally, “dummy” should express “TRA-1-81”, “SSEA4” at the end of culture.

Protocol for efficient generation of human artery and vein endothelial cells from pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

Protocol for efficient generation of human artery and vein endothelial cells from pluripotent stem cells

DOI: 10.1016/j.xpro.2024.103494

Authors: Kyle M. Loh; Sherry Li Zheng; Kevin J. Liu; Qingqing Yin; Zhainib A. Amir-Ugokwe; Sawan K. Jha; Yue Qi; Vanessa K. Wazny; Alana T. Nguyen; Angela Chen; Faith-Masong Njunkeng; Christine Cheung; Edda Spiekerkoetter; Kristy Red-Horse; Lay Teng Ang

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “mTeSR Plus” for “1” days. For ECM, use “Geltrex” at “1” “%”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Protocol to generate endothelial cells, pericytes, and fibroblasts in one differentiation round from human-induced pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

Protocol to generate endothelial cells, pericytes, and fibroblasts in one differentiation round from human-induced pluripotent stem cells

DOI: 10.1016/j.xpro.2023.102292

Authors: Tea Soon Park; Rishabh Hirday; Amir Ali; Roba Megersa; Rafael Villasmil; Eric Nguyen; Kapil Bharti

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “E8”. For ECM, use “vitronectin” at “1” “%”. Medium should contain factors shown in the following table.

PU.1 drives specification of pluripotent stem cell-derived endothelial cells to LSEC-like cells

Mon, 01 Jan 0001 00:00:00 +0000

PU.1 drives specification of pluripotent stem cell-derived endothelial cells to LSEC-like cells

DOI: 10.1038/s41419-020-03356-2

Authors: Jonathan De Smedt; Elise Anne van Os; Irene Talon; Sreya Ghosh; Burak Toprakhisar; Rodrigo Furtado Madeiro Da Costa; Samantha Zaunz; Marta Aguirre Vazquez; Ruben Boon; Pieter Baatsen; Ayla Smout; Stefaan Verhulst; Leo A. van Grunsven; Catherine M. Verfaillie

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human ESC in “E8 Flex medium”. Medium should contain factors shown in the following table.

RAAS-deficient organoids indicate delayed angiogenesis as a possible cause for autosomal recessive renal tubular dysgenesis

Mon, 01 Jan 0001 00:00:00 +0000

RAAS-deficient organoids indicate delayed angiogenesis as a possible cause for autosomal recessive renal tubular dysgenesis

DOI: 10.1038/s41467-023-43795-x

Authors: Naomi Pode-Shakked; Megan Slack; Nambirajan Sundaram; Ruth Schreiber; Kyle W. McCracken; Benjamin Dekel; Michael Helmrath; Raphael Kopan

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC and human PSC in “mTeSR” for “1” days. “PSC” should be “downregulated” for “ACE”. “PSC” should be “downregulated” for “AGTR1”. For ECM, use “Cultrex” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Reactive astrocytes transduce inflammation in a blood-brain barrier model through a TNF-STAT3 signaling axis and secretion of alpha 1-antichymotrypsin

Mon, 01 Jan 0001 00:00:00 +0000

Reactive astrocytes transduce inflammation in a blood-brain barrier model through a TNF-STAT3 signaling axis and secretion of alpha 1-antichymotrypsin

DOI: 10.1038/s41467-022-34412-4

Authors: Hyosung Kim; Kun Leng; Jinhee Park; Alexander G. Sorets; Suil Kim; Alena Shostak; Rebecca J. Embalabala; Kate Mlouk; Ketaki A. Katdare; Indigo V. L. Rose; Sarah M. Sturgeon; Emma H. Neal; Yan Ao; Shinong Wang; Michael V. Sofroniew; Jonathan M. Brunger; Douglas G. McMahon; Matthew S. Schrag; Martin Kampmann; Ethan S. Lippmann

Reconstituted basement membrane enables airway epithelium modeling and nanoparticle toxicity testing

Mon, 01 Jan 0001 00:00:00 +0000

Reconstituted basement membrane enables airway epithelium modeling and nanoparticle toxicity testing

DOI: 10.1016/j.ijbiomac.2022.02.018

Authors: Elrade Rofaani; Boxin Huang; Feng Liang; Juan Peng; Yong Chen

Step 1: iPSC/ESC maintainance

day 0 ~ 2

culture “adherent” human PSC in “E8 Flex medium” for “2” days. For ECM, use “vitronectin” at “1” “%”. Medium should be changed once in “2” days.

Ideally, “PSC” should be obtained at the end of culture.

Regenerative potential of induced pluripotent stem cells derived from patients undergoing haemodialysis in kidney regeneration

Mon, 01 Jan 0001 00:00:00 +0000

Regenerative potential of induced pluripotent stem cells derived from patients undergoing haemodialysis in kidney regeneration

DOI: 10.1038/s41598-018-33256-7

Authors: Susumu Tajiri; Shuichiro Yamanaka; Toshinari Fujimoto; Kei Matsumoto; Atsuhiro Taguchi; Ryuichi Nishinakamura; Hirotaka James Okano; Takashi Yokoo

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” human PSC in “unspecified medium”.

Ideally, “PSC” should be obtained at the end of culture.

Renal Subcapsular Transplantation of PSC-Derived Kidney Organoids Induces Neo-vasculogenesis and Significant Glomerular and Tubular Maturation In Vivo

Mon, 01 Jan 0001 00:00:00 +0000

Renal Subcapsular Transplantation of PSC-Derived Kidney Organoids Induces Neo-vasculogenesis and Significant Glomerular and Tubular Maturation In Vivo

DOI: 10.1016/j.stemcr.2018.01.041

Authors: Cathelijne W. van den Berg; Laila Ritsma; M. Cristina Avramut; Loes E. Wiersma; Bernard M. van den Berg; Daniëlle G. Leuning; Ellen Lievers; Marije Koning; Jessica M. Vanslambrouck; Abraham J. Koster; Sara E. Howden; Minoru Takasato; Melissa H. Little; Ton J. Rabelink

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “E8” for “1” days. For ECM, use “vitronectin” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Repair of Ischemic Injury by Pluripotent Stem Cell Based Cell Therapy without Teratoma through Selective Photosensitivity

Mon, 01 Jan 0001 00:00:00 +0000

Repair of Ischemic Injury by Pluripotent Stem Cell Based Cell Therapy without Teratoma through Selective Photosensitivity

DOI: 10.1016/j.stemcr.2015.10.004

Authors: Seung-Ju Cho; So-Yeon Kim; Ho-Chang Jeong; Hyeonsik Cheong; Doseok Kim; Soon-Jung Park; Jong-Jin Choi; Hyongbum Kim; Hyung-Min Chung; Sung-Hwan Moon; Hyuk-Jin Cha

Step 1: Primitive streak 1

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “spheroid” mouse ESC in “unspecified medium”.

Step 2: Primitive streak 2

day 1 ~ 5

Restricted Presence of POU6F2 in Human Corneal Endothelial Cells Uncovered by Extension of the Promoter-level Expression Atlas

Mon, 01 Jan 0001 00:00:00 +0000

Restricted Presence of POU6F2 in Human Corneal Endothelial Cells Uncovered by Extension of the Promoter-level Expression Atlas

DOI: 10.1016/j.ebiom.2017.10.024

Authors: Masahito Yoshihara; Susumu Hara; Motokazu Tsujikawa; Satoshi Kawasaki; Yoshihide Hayashizaki; Masayoshi Itoh; Hideya Kawaji; Kohji Nishida

Step 1: endothelial 1

day 0 ~ 28

culture “adherent” human HCEP in “DMEM” for “28” days. For ECM, use “FNC coating mix” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “28” days.

Retrotransposons in Werner syndrome-derived macrophages trigger type I interferon-dependent inflammation in an atherosclerosis model

Mon, 01 Jan 0001 00:00:00 +0000

Retrotransposons in Werner syndrome-derived macrophages trigger type I interferon-dependent inflammation in an atherosclerosis model

DOI: 10.1038/s41467-024-48663-w

Authors: Sudip Kumar Paul; Motohiko Oshima; Ashwini Patil; Masamitsu Sone; Hisaya Kato; Yoshiro Maezawa; Hiyori Kaneko; Masaki Fukuyo; Bahityar Rahmutulla; Yasuo Ouchi; Kyoko Tsujimura; Mahito Nakanishi; Atsushi Kaneda; Atsushi Iwama; Koutaro Yokote; Koji Eto; Naoya Takayama

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “AK02N medium”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table.

Robust differentiation of human pluripotent stem cells into mural progenitor cells via transient activation of NKX3.1

Mon, 01 Jan 0001 00:00:00 +0000

Robust differentiation of human pluripotent stem cells into mural progenitor cells via transient activation of NKX3.1

DOI: 10.1038/s41467-024-52678-8

Authors: Umji Lee; Yadong Zhang; Yonglin Zhu; Allen Chilun Luo; Liyan Gong; Daniel M. Tremmel; Yunhye Kim; Victoria Sofia Villarreal; Xi Wang; Ruei-Zeng Lin; Miao Cui; Minglin Ma; Ke Yuan; Kai Wang; Kaifu Chen; Juan M. Melero-Martin

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “mTeSR Plus” for “1” days. “PSC” should be “upregulated” for “NKX3.1”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Robust Revascularization in Models of Limb Ischemia Using a Clinically Translatable Human Stem Cell-Derived Endothelial Cell Product

Mon, 01 Jan 0001 00:00:00 +0000

Robust Revascularization in Models of Limb Ischemia Using a Clinically Translatable Human Stem Cell-Derived Endothelial Cell Product

DOI: 10.1016/j.ymthe.2018.03.017

Authors: Mark G. MacAskill; Jaimy Saif; Alison Condie; Maurits A. Jansen; Thomas J. MacGillivray; Adriana A.S. Tavares; Lucija Fleisinger; Helen L. Spencer; Marie Besnier; Ernesto Martin; Giovanni Biglino; David E. Newby; Patrick W.F. Hadoke; Joanne C. Mountford; Costanza Emanueli; Andrew H. Baker

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

Role of Mir-155 in Controlling HIF-1α Level and Promoting Endothelial Cell Maturation

Mon, 01 Jan 0001 00:00:00 +0000

Role of Mir-155 in Controlling HIF-1α Level and Promoting Endothelial Cell Maturation

DOI: 10.1038/srep35316

Authors: Deguang Yang; Jinhong Wang; Meng Xiao; Tao Zhou; Xu Shi

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “DMEM/F12”. For ECM, use “Fibroblast” at “N/A” “N/A”. Medium should contain factors shown in the following table.

	concentration	unit
KSR	20	%
NEAA	—	—
Glutamine	0.002	mol/l
2-mercaptoethanol	1.0e-4	mol/l
FGF	2.0e-5	g/l
PSA	1	%

Ideally, “PSC” should be obtained at the end of culture.

Role of Notch1 in the arterial specification and angiogenic potential of mouse embryonic stem cell-derived endothelial cells

Mon, 01 Jan 0001 00:00:00 +0000

Role of Notch1 in the arterial specification and angiogenic potential of mouse embryonic stem cell-derived endothelial cells

DOI: 10.1186/s13287-018-0945-7

Authors: Jae Kyung Park; Tae Wook Lee; Eun Kyoung Do; Hye Ji Moon; Jae Ho Kim

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” mouse ESC in “DMEM”. For ECM, use “Fibroblast” at “N/A” “N/A”. Medium should contain factors shown in the following table.

Role of YAP in hematopoietic differentiation and erythroid lineage specification of human-induced pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

Role of YAP in hematopoietic differentiation and erythroid lineage specification of human-induced pluripotent stem cells

DOI: 10.1186/s13287-023-03508-z

Authors: Chuti Laowtammathron; Chanchao Lorthongpanich; Nittaya Jiamvoraphong; Pimonwan Srisook; Phatchanat Klaihmon; Pakpoom Kheolamai; Sudjit Luanpitpong; Surapol Issaragrisil

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC and human PSC and human PSC in “Nutristem medium”. “PSC” should be “downregulated” for “YAP1”. “PSC” should be “upregulated” for “YAP1”. For ECM, use “Matrigel” at “N/A” “N/A”.

Safety and efficacy of human ESC-derived corneal endothelial cells for corneal endothelial dysfunction

Mon, 01 Jan 0001 00:00:00 +0000

Safety and efficacy of human ESC-derived corneal endothelial cells for corneal endothelial dysfunction

DOI: 10.1186/s13578-023-01145-w

Authors: Juan Yu; Nianye Yu; Yao Tian; Yifan Fang; Bin An; Guihai Feng; Jun Wu; Liu Wang; Jie Hao; Liqiang Wang; Qi Zhou; Wei Li; Yukai Wang; Baoyang Hu

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human ESC in “E8” for “1” days. For ECM, use “vitronectin” at “N/A” “N/A”. Medium should be changed once in “1” days.

Sall4 and Gata4 induce cardiac fibroblast transition towards a partially multipotent state with cardiogenic potential

Mon, 01 Jan 0001 00:00:00 +0000

Sall4 and Gata4 induce cardiac fibroblast transition towards a partially multipotent state with cardiogenic potential

DOI: 10.1038/s41598-024-73975-8

Authors: Hong Gao; Saliha Pathan; Beverly R. E. A. Dixon; Aarthi Pugazenthi; Megumi Mathison; Tamer M.A. Mohamed; Todd K. Rosengart; Jianchang Yang

Step 1: endothelial 1

day 0 ~ 7

culture “unspecified method” unknown species cell in “EGM2” for “7” days. Medium should contain factors shown in the following table, and should be changed once in “7” days.

SARS-CoV-2 infection of human pluripotent stem cell-derived vascular cells reveals smooth muscle cells as key mediators of vascular pathology during infection

Mon, 01 Jan 0001 00:00:00 +0000

SARS-CoV-2 infection of human pluripotent stem cell-derived vascular cells reveals smooth muscle cells as key mediators of vascular pathology during infection

DOI: 10.1038/s41467-024-54917-4

Authors: Alexsia Richards; Andrew S. Khalil; Max Friesen; Troy W. Whitfield; Xinlei Gao; Tenzin Lungjangwa; Roger D. Kamm; Zhengpeng Wan; Lee Gehrke; David Mooney; Rudolf Jaenisch

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “E8”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table.

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Mon, 01 Jan 0001 00:00:00 +0000

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iPSC ESC Maintainance / result

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Self-assembling 3D vessel-on-chip model with hiPSC-derived astrocytes

Mon, 01 Jan 0001 00:00:00 +0000

Self-assembling 3D vessel-on-chip model with hiPSC-derived astrocytes

DOI: 10.1016/j.stemcr.2024.05.006

Authors: Dennis M. Nahon; Marc Vila Cuenca; Francijna E. van den Hil; Michel Hu; Tessa de Korte; Jean-Philippe Frimat; Arn M.J.M. van den Maagdenberg; Christine L. Mummery; Valeria V. Orlova

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” human PSC in “unspecified medium”.

Ideally, “PSC” should be obtained at the end of culture.

SETD7 promotes lateral plate mesoderm formation by modulating the Wnt/β-catenin signaling pathway

Mon, 01 Jan 0001 00:00:00 +0000

SETD7 promotes lateral plate mesoderm formation by modulating the Wnt/β-catenin signaling pathway

DOI: 10.1016/j.isci.2023.106917

Authors: Ding Wang; Yapu Li; Changlu Xu; Hongtao Wang; Xin Huang; Xu Jin; Sirui Ren; Jie Gao; Jingyuan Tong; Jinhua Liu; Jiaxi Zhou; Lihong Shi

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human ESC in “mTeSR”. “ESC” should be “downregulated” for “SETD7”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table.

Sex-dependent VEGF expression underlies variations in human pluripotent stem cell to endothelial progenitor differentiation

Mon, 01 Jan 0001 00:00:00 +0000

Sex-dependent VEGF expression underlies variations in human pluripotent stem cell to endothelial progenitor differentiation

DOI: 10.1038/s41598-019-53054-z

Authors: Lauren N. Randolph; Xiaoping Bao; Michael Oddo; Xiaojun Lance Lian

Step 1: iPSC/ESC maintainance

day 0 ~ 2

culture “adherent” human PSC in “LaSR medium” for “2” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

	concentration	unit
Y-27623	5.0e-6	mol/l

Step 2: Primitive streak 1

day 2 ~ 3

Simultaneous induction of vasculature and neuronal network formation on a chip reveals a dynamic interrelationship between cell types

Mon, 01 Jan 0001 00:00:00 +0000

Simultaneous induction of vasculature and neuronal network formation on a chip reveals a dynamic interrelationship between cell types

DOI: 10.1186/s12964-023-01159-4

Authors: Lotta Isosaari; Hanna Vuorenpää; Alma Yrjänäinen; Fikret Emre Kapucu; Minna Kelloniemi; Toni-Karri Pakarinen; Susanna Miettinen; Susanna Narkilahti

No steps available

Single cell RNA sequencing of human liver reveals distinct intrahepatic macrophage populations

Mon, 01 Jan 0001 00:00:00 +0000

Single cell RNA sequencing of human liver reveals distinct intrahepatic macrophage populations

DOI: 10.1038/s41467-018-06318-7

Authors: Sonya A. MacParland; Jeff C. Liu; Xue-Zhong Ma; Brendan T. Innes; Agata M. Bartczak; Blair K. Gage; Justin Manuel; Nicholas Khuu; Juan Echeverri; Ivan Linares; Rahul Gupta; Michael L. Cheng; Lewis Y. Liu; Damra Camat; Sai W. Chung; Rebecca K. Seliga; Zigong Shao; Elizabeth Lee; Shinichiro Ogawa; Mina Ogawa; Michael D. Wilson; Jason E. Fish; Markus Selzner; Anand Ghanekar; David Grant; Paul Greig; Gonzalo Sapisochin; Nazia Selzner; Neil Winegarden; Oyedele Adeyi; Gordon Keller; Gary D. Bader; Ian D. McGilvray

Single-cell clones of liver cancer stem cells have the potential of differentiating into different types of tumor cells

Mon, 01 Jan 0001 00:00:00 +0000

Single-cell clones of liver cancer stem cells have the potential of differentiating into different types of tumor cells

DOI: 10.1038/cddis.2013.340

Authors: H Liu; W Zhang; Y Jia; Q Yu; G E Grau; L Peng; Y Ran; Z Yang; H Deng; J Lou

No steps available

Single-cell transcriptomic analysis of corneal organoids during development

Mon, 01 Jan 0001 00:00:00 +0000

Single-cell transcriptomic analysis of corneal organoids during development

DOI: 10.1016/j.stemcr.2023.10.022

Authors: Aditi Swarup; Ragini Phansalkar; Maurizio Morri; Aditi Agarwal; Varun Subramaniam; BaoXiang Li; Albert Y. Wu

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” human PSC in “unspecified medium”.

Step 2: mesoderm 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

culture “unspecified method” unknown species cell in “unspecified medium”.

SOX17/ETV2 improves the direct reprogramming of adult fibroblasts to endothelial cells

Mon, 01 Jan 0001 00:00:00 +0000

SOX17/ETV2 improves the direct reprogramming of adult fibroblasts to endothelial cells

DOI: 10.1016/j.crmeth.2024.100732

Authors: Alexander Grath; Guohao Dai

Step 1: endothelial 1

day 0 ~ 2

culture “adherent” unknown species cell and human Fibroblast in “EGM2” for “2” days. For ECM, use “Gelatin” at “2” “%”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

	concentration	unit
Growth Medium 2 Supplement Mix	—	—
PSA	1	%
GlutaMax	1	%
doxycycline	0.001	g/l

Ideally, “EC” should express “VE-CAD”, “PECAM1”, “VWF”, “EphrinB2” at the end of culture. Ideally, “EC” should express “ETV2”, “SOX17” at the end of culture.

SOX18-enforced expression diverts hemogenic endothelium-derived progenitors from T towards NK lymphoid pathways

Mon, 01 Jan 0001 00:00:00 +0000

SOX18-enforced expression diverts hemogenic endothelium-derived progenitors from T towards NK lymphoid pathways

DOI: 10.1016/j.isci.2023.106621

Authors: Ho Sun Jung; Kran Suknuntha; Yun Hee Kim; Peng Liu; Samuel T. Dettle; Divine Mensah Sedzro; Portia R. Smith; James A. Thomson; Irene M. Ong; Igor I. Slukvin

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human cell in “E8” for “1” days. For ECM, use “Collagen IV” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Specification and Diversification of Pericytes and Smooth Muscle Cells from Mesenchymoangioblasts

Mon, 01 Jan 0001 00:00:00 +0000

Specification and Diversification of Pericytes and Smooth Muscle Cells from Mesenchymoangioblasts

DOI: 10.1016/j.celrep.2017.05.019

Authors: Akhilesh Kumar; Saritha Sandra D’Souza; Oleg V. Moskvin; Huishi Toh; Bowen Wang; Jue Zhang; Scott Swanson; Lian-Wang Guo; James A. Thomson; Igor I. Slukvin

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “unspecified medium”. For ECM, use “mouse embryonic fibroblasts” at “N/A” “N/A”.

Step 2: mesoderm 1

day 1 ~ 3

SPI1-KLF1/LYL1 axis regulates lineage commitment during endothelial-to-hematopoietic transition from human pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

SPI1-KLF1/LYL1 axis regulates lineage commitment during endothelial-to-hematopoietic transition from human pluripotent stem cells

DOI: 10.1016/j.isci.2024.110409

Authors: Kengyuan Qu; Shaokang Mo; Junfeng Huang; Shan Liu; Shuo Zhang; Jun Shen; Kuangyu Yen

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human ESC in “E8” for “1” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

SREBP1 suppresses the differentiation and epithelial function of hiPSC-derived endothelial cells by inhibiting the microRNA199b-5p pathway

Mon, 01 Jan 0001 00:00:00 +0000

SREBP1 suppresses the differentiation and epithelial function of hiPSC-derived endothelial cells by inhibiting the microRNA199b-5p pathway

DOI: 10.1016/j.scr.2021.102174

Authors: Xin Qian; Xiangjiang Guo; Qihong Ni; Han Wang; Meng Ye; Lan Zhang

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “Matrigel” at “N/A” “N/A”.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: Primitive streak 1

day 1 ~ 4

Stable engineered vascular networks from human induced pluripotent stem cell-derived endothelial cells cultured in synthetic hydrogels

Mon, 01 Jan 0001 00:00:00 +0000

Stable engineered vascular networks from human induced pluripotent stem cell-derived endothelial cells cultured in synthetic hydrogels

DOI: 10.1016/j.actbio.2016.03.001

Authors: Matthew R. Zanotelli; Hamisha Ardalani; Jue Zhang; Zhonggang Hou; Eric H. Nguyen; Scott Swanson; Bao Kim Nguyen; Jennifer Bolin; Angela Elwell; Lauren L. Bischel; Angela W. Xie; Ron Stewart; David J. Beebe; James A. Thomson; Michael P. Schwartz; William L. Murphy

No steps available

Stepwise differentiation and functional characterization of human induced pluripotent stem cell-derived choroidal endothelial cells

Mon, 01 Jan 0001 00:00:00 +0000

Stepwise differentiation and functional characterization of human induced pluripotent stem cell-derived choroidal endothelial cells

DOI: 10.1186/s13287-020-01903-4

Authors: Kelly Mulfaul; Joseph C. Giacalone; Andrew P. Voigt; Megan J. Riker; Dalyz Ochoa; Ian C. Han; Edwin M. Stone; Robert F. Mullins; Budd A. Tucker

Step 1: iPSC/ESC maintainance

day 0 ~ 1

Targeted CRISPR activation is functional in engineered human pluripotent stem cells but undergoes silencing after differentiation into cardiomyocytes and endothelium

Mon, 01 Jan 0001 00:00:00 +0000

Targeted CRISPR activation is functional in engineered human pluripotent stem cells but undergoes silencing after differentiation into cardiomyocytes and endothelium

DOI: 10.1007/s00018-023-05101-2

Authors: Elaheh Karbassi; Ruby Padgett; Alessandro Bertero; Hans Reinecke; Jordan M. Klaiman; Xiulan Yang; Stephen D. Hauschka; Charles E. Murry

Step 1: iPSC/ESC maintainance

day 0 ~ 1

Targeted proteomics for absolute quantification of glucose transporter 1 in mammalian brain cells using liquid chromatography-mass spectrometry

Mon, 01 Jan 0001 00:00:00 +0000

Targeted proteomics for absolute quantification of glucose transporter 1 in mammalian brain cells using liquid chromatography-mass spectrometry

DOI: 10.1016/j.jcoa.2024.100198

Authors: Yash Mehta; Dhavalkumar Patel; Iqra Pervaiz; Ulrich Bickel; Abraham Jacob Al-Ahmad

Step 1: iPSC/ESC maintainance

day 0 ~ 4

culture “adherent” human PSC in “E8” for “4” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should be changed once in “4” days.

Ideally, “PSC” should be obtained at the end of culture.

TGF-β1-induced differentiation of SHED into functional smooth muscle cells

Mon, 01 Jan 0001 00:00:00 +0000

TGF-β1-induced differentiation of SHED into functional smooth muscle cells

DOI: 10.1186/s13287-016-0459-0

Authors: Jian Guang Xu; Shao Yue Zhu; Boon Chin Heng; Waruna Lakmal Dissanayaka; Cheng Fei Zhang

No steps available

The ACVR1 R206H mutation found in fibrodysplasia ossificans progressiva increases human induced pluripotent stem cell-derived endothelial cell formation and collagen production through BMP-mediated SMAD1/5/8 signaling

Mon, 01 Jan 0001 00:00:00 +0000

The ACVR1 R206H mutation found in fibrodysplasia ossificans progressiva increases human induced pluripotent stem cell-derived endothelial cell formation and collagen production through BMP-mediated SMAD1/5/8 signaling

DOI: 10.1186/s13287-016-0372-6

Authors: Emilie Barruet; Blanca M. Morales; Wint Lwin; Mark P. White; Christina V. Theodoris; Hannah Kim; Ashley Urrutia; Sarah Anne Wong; Deepak Srivastava; Edward C. Hsiao

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. “PSC” should be “unspecified regulation” for “ACVR1”. For ECM, use “Matrigel” at “0.15” “g/l”. Medium should contain factors shown in the following table.

The effect of estrogen on diabetic wound healing is mediated through increasing the function of various bone marrow-derived progenitor cells

Mon, 01 Jan 0001 00:00:00 +0000

The effect of estrogen on diabetic wound healing is mediated through increasing the function of various bone marrow-derived progenitor cells

DOI: 10.1016/j.jvs.2018.04.069

Authors: Ying Zhuge; Manuela M. Regueiro; Runxia Tian; Yan Li; Xiaomei Xia; Roberto Vazquez-Padron; Sharon Elliot; Seth R. Thaller; Zhao-Jun Liu; Omaida C. Velazquez

No steps available

The growth of endothelial-like cells in zebrafish embryoid body culture

Mon, 01 Jan 0001 00:00:00 +0000

The growth of endothelial-like cells in zebrafish embryoid body culture

DOI: 10.1016/j.yexcr.2020.112032

Authors: Muhammad Ibrahim; Bing Xie; Michael K. Richardson

Step 1: endothelial 1

day 0 ~ 4

culture “spheroid” zebrafish cell in “LDF medium” for “4” days. Medium should contain factors shown in the following table, and should be changed once in “4” days.

	concentration	unit
EGS	4.1	%
FBS	20	%
ZEE	0.05	g/l
VEGF	4.0e-5	g/l
FGF	1.0e-5	g/l

Ideally, “EC” should express “fli1”, “kdrl” at the end of culture.

The histone lysine methyltransferase SETD8 regulates angiogenesis through HES-1 in human umbilical vein endothelial cells

Mon, 01 Jan 0001 00:00:00 +0000

The histone lysine methyltransferase SETD8 regulates angiogenesis through HES-1 in human umbilical vein endothelial cells

DOI: 10.1038/s41598-020-69103-x

Authors: Dong Kyu Choi; Young Kyu Kim; Sang Wook Park; Heejin Lee; Seul Lee; Sang A. Kim; Soo Jin Kim; Junyeop Lee; Wanil Kim; Sang-Hyun Min; Ji Hoon Yu

Step 1: iPSC/ESC maintainance

day 0 ~ 1

The influence of a spatiotemporal 3D environment on endothelial cell differentiation of human induced pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

The influence of a spatiotemporal 3D environment on endothelial cell differentiation of human induced pluripotent stem cells

DOI: 10.1016/j.biomaterials.2014.01.037

Authors: Sophia Zhang; James R. Dutton; Liping Su; Jianyi Zhang; Lei Ye

Step 1: iPSC/ESC maintainance

day 0 ~ 2

culture “spheroid” human PSC in “unspecified medium” for “2” days. “PSC” should be “upregulated” for “GFP”. For ECM, use “fibrin” at “N/A” “N/A”. Medium should be changed once in “2” days.

Step 2: mesoderm 1

day 2 ~ 3

The method to generate pulsatile circulatory fluid flow using microfluidics

Mon, 01 Jan 0001 00:00:00 +0000

The method to generate pulsatile circulatory fluid flow using microfluidics

DOI: 10.1016/j.mex.2021.101269

Authors: Jingjing Li; Osmond Lao; Robert E. Nordon

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human ESC in “E8”. For ECM, use “Geltrex” at “1” “%”.

Ideally, “ESC” should be obtained at the end of culture.

The miR-148/152 family contributes to angiogenesis of human pluripotent stem cell- derived endothelial cells by inhibiting MEOX2

Mon, 01 Jan 0001 00:00:00 +0000

The miR-148/152 family contributes to angiogenesis of human pluripotent stem cell- derived endothelial cells by inhibiting MEOX2

DOI: 10.1016/j.omtn.2023.04.020

Authors: Fengyue Ding; Hongchun Wu; Xinglong Han; Xue Jiang; Yang Xiao; Yuanyuan Tu; Miao Yu; Wei Lei; Shijun Hu

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human ESC in “PSCeasy medium”. “ESC” should be “downregulated” for “miR-148/152”. For ECM, use “Matrigel” at “N/A” “N/A”.

The modulation of endothelial cell morphology, function, and survival using anisotropic nanofibrillar collagen scaffolds

Mon, 01 Jan 0001 00:00:00 +0000

The modulation of endothelial cell morphology, function, and survival using anisotropic nanofibrillar collagen scaffolds

DOI: 10.1016/j.biomaterials.2013.02.036

Authors: Ngan F. Huang; Janet Okogbaa; Jerry C. Lee; Arshi Jha; Tatiana S. Zaitseva; Michael V. Paukshto; John S. Sun; Niraj Punjya; Gerald G. Fuller; John P. Cooke

No steps available

The prostaglandin H2 analog U-46619 improves the differentiation efficiency of human induced pluripotent stem cells into endothelial cells by activating both p38MAPK and ERK1/2 signaling pathways

Mon, 01 Jan 0001 00:00:00 +0000

The prostaglandin H2 analog U-46619 improves the differentiation efficiency of human induced pluripotent stem cells into endothelial cells by activating both p38MAPK and ERK1/2 signaling pathways

DOI: 10.1186/s13287-018-1061-4

Authors: Liping Su; Xiaocen Kong; Szeyun Lim; Szejie Loo; Shihua Tan; Kiankeong Poh; James Dutton; Colin Stewart; Stuart Cook; Xiaofei Su; Jianhua Ma; Jianyi Zhang; Lei Ye

Step 1: iPSC/ESC maintainance

day 0 ~ 4

culture “spheroid” human PSC in “E8” for “4” days. Medium should contain factors shown in the following table, and should be changed once in “4” days.

The role of mutations associated with familial neurodegenerative disorders on blood–brain barrier function in an iPSC model

Mon, 01 Jan 0001 00:00:00 +0000

The role of mutations associated with familial neurodegenerative disorders on blood–brain barrier function in an iPSC model

DOI: 10.1186/s12987-019-0139-4

Authors: Moriah E. Katt; Lakyn N. Mayo; Shannon E. Ellis; Vasiliki Mahairaki; Jeffrey D. Rothstein; Linzhao Cheng; Peter C. Searson

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “E8” for “1” days. For ECM, use “Vitronectin” at “N/A” “N/A”. Medium should be changed once in “1” days.

Therapeutic angiogenesis by transplantation of induced pluripotent stem cell-derived Flk-1 positive cells

Mon, 01 Jan 0001 00:00:00 +0000

Therapeutic angiogenesis by transplantation of induced pluripotent stem cell-derived Flk-1 positive cells

DOI: 10.1186/1471-2121-11-72

Authors: Hirohiko Suzuki; Rei Shibata; Tetsutaro Kito; Masakazu Ishii; Ping Li; Toru Yoshikai; Naomi Nishio; Sachiko Ito; Yasushi Numaguchi; Jun K Yamashita; Toyoaki Murohara; Kenichi Isobe

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” mouse PSC in “DMEM”. For ECM, use “MEF feeder” at “N/A” “N/A”. Medium should contain factors shown in the following table.

Therapeutic correction of hemophilia A by transplantation of hPSC-derived liver sinusoidal endothelial cell progenitors

Mon, 01 Jan 0001 00:00:00 +0000

Therapeutic correction of hemophilia A by transplantation of hPSC-derived liver sinusoidal endothelial cell progenitors

DOI: 10.1016/j.celrep.2022.110621

Authors: Blair K. Gage; Simone Merlin; Cristina Olgasi; Antonia Follenzi; Gordon M. Keller

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “DMEM/F12”. For ECM, use “Matrigel” at “0.25” “%”. Medium should contain factors shown in the following table.

	concentration	unit
KSR	—	—
FGF	1.0e-5	g/l
Y-27623	1.0e-5	mol/l

Step 2: mesoderm 1

day 1 ~ 4

Three-Dimensional Human iPSC-Derived Artificial Skeletal Muscles Model Muscular Dystrophies and Enable Multilineage Tissue Engineering

Mon, 01 Jan 0001 00:00:00 +0000

Three-Dimensional Human iPSC-Derived Artificial Skeletal Muscles Model Muscular Dystrophies and Enable Multilineage Tissue Engineering

DOI: 10.1016/j.celrep.2018.03.091

Authors: Sara Martina Maffioletti; Shilpita Sarcar; Alexander B.H. Henderson; Ingra Mannhardt; Luca Pinton; Louise Anne Moyle; Heather Steele-Stallard; Ornella Cappellari; Kim E. Wells; Giulia Ferrari; Jamie S. Mitchell; Giulia E. Tyzack; Vassilios N. Kotiadis; Moustafa Khedr; Martina Ragazzi; Weixin Wang; Michael R. Duchen; Rickie Patani; Peter S. Zammit; Dominic J. Wells; Thomas Eschenhagen; Francesco Saverio Tedesco

Tissue-engineered blood-brain barrier models via directed differentiation of human induced pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

Tissue-engineered blood-brain barrier models via directed differentiation of human induced pluripotent stem cells

DOI: 10.1038/s41598-019-50193-1

Authors: Gabrielle N. Grifno; Alanna M. Farrell; Raleigh M. Linville; Diego Arevalo; Joo Ho Kim; Luo Gu; Peter C. Searson

Step 1: iPSC/ESC maintainance

day 0 ~ 3

culture “adherent” human PSC and human PSC in “mTeSR” for “3” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “3” days.

Tracing the first hematopoietic stem cell generation in human embryo by single-cell RNA sequencing

Mon, 01 Jan 0001 00:00:00 +0000

Tracing the first hematopoietic stem cell generation in human embryo by single-cell RNA sequencing

DOI: 10.1038/s41422-019-0228-6

Authors: Yang Zeng; Jian He; Zhijie Bai; Zongcheng Li; Yandong Gong; Chen Liu; Yanli Ni; Junjie Du; Chunyu Ma; Lihong Bian; Yu Lan; Bing Liu

No steps available

Transcriptionally and Functionally Distinct Mesenchymal Subpopulations Are Generated from Human Pluripotent Stem Cells

Mon, 01 Jan 0001 00:00:00 +0000

Transcriptionally and Functionally Distinct Mesenchymal Subpopulations Are Generated from Human Pluripotent Stem Cells

DOI: 10.1016/j.stemcr.2017.12.005

Authors: Chee Jia Chin; Suwen Li; Mirko Corselli; David Casero; Yuhua Zhu; Chong Bin He; Reef Hardy; Bruno Péault; Gay M. Crooks

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human ESC in “unspecified medium”.

Step 2: mesoderm 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

Transcriptome profiling of 3D co-cultured cardiomyocytes and endothelial cells under oxidative stress using a photocrosslinkable hydrogel system

Mon, 01 Jan 0001 00:00:00 +0000

Transcriptome profiling of 3D co-cultured cardiomyocytes and endothelial cells under oxidative stress using a photocrosslinkable hydrogel system

DOI: 10.1016/j.actbio.2017.06.031

Authors: Xiaoshan Yue; Aylin Acun; Pinar Zorlutuna

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “mTeSR” for “1” days. For ECM, use “Geltrex” at “N/A” “N/A”. Medium should be changed once in “1” days.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: endothelial 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

Transcriptomically Guided Mesendoderm Induction of Human Pluripotent Stem Cells Using a Systematically Defined Culture Scheme

Mon, 01 Jan 0001 00:00:00 +0000

Transcriptomically Guided Mesendoderm Induction of Human Pluripotent Stem Cells Using a Systematically Defined Culture Scheme

DOI: 10.1016/j.stemcr.2019.11.001

Authors: Richard L. Carpenedo; Sarah Y. Kwon; R. Matthew Tanner; Julien Yockell-Lelièvre; Chandarong Choey; Carole Doré; Mirabelle Ho; Duncan J. Stewart; Theodore J. Perkins; William L. Stanford

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “E8”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table.

Transdifferentiated Human Vascular Smooth Muscle Cells are a New Potential Cell Source for Endothelial Regeneration

Mon, 01 Jan 0001 00:00:00 +0000

Transdifferentiated Human Vascular Smooth Muscle Cells are a New Potential Cell Source for Endothelial Regeneration

DOI: 10.1038/s41598-017-05665-7

Authors: Xuechong Hong; Andriana Margariti; Alexandra Le Bras; Laureen Jacquet; Wei Kong; Yanhua Hu; Qingbo Xu

Step 1: Primitive streak 1

day 0 ~ 2

culture “adherent” human Pericyte/SMC in “KnockOut DMEM/F12” for “2” days. “Pericyte/SMC” should be “upregulated” for “POU5F1”, “upregulated” for “SOX2”, “upregulated” for “KLF4”, “upregulated” for “c-MYC”. For ECM, use “Gelatin” at “0.05” “%”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Transdifferentiation of human endothelial progenitors into smooth muscle cells

Mon, 01 Jan 0001 00:00:00 +0000

Transdifferentiation of human endothelial progenitors into smooth muscle cells

DOI: 10.1016/j.biomaterials.2016.01.066

Authors: HaYeun Ji; Leigh Atchison; Zaozao Chen; Syandan Chakraborty; Youngmee Jung; George A. Truskey; Nicolas Christoforou; Kam W. Leong

No steps available

Transient Changes in Oxygen Tension Inhibit Osteogenic Differentiation and Runx2 Expression in Osteoblasts

Mon, 01 Jan 0001 00:00:00 +0000

Transient Changes in Oxygen Tension Inhibit Osteogenic Differentiation and Runx2 Expression in Osteoblasts

DOI: 10.1074/jbc.M403715200

Authors: Ali Salim; Randall P. Nacamuli; Elise F. Morgan; Amato J. Giaccia; Michael T. Longaker

No steps available

Transient secretion of VEGF protein from transplanted hiPSC-CMs enhances engraftment and improves rat heart function post MI

Mon, 01 Jan 0001 00:00:00 +0000

Transient secretion of VEGF protein from transplanted hiPSC-CMs enhances engraftment and improves rat heart function post MI

DOI: 10.1016/j.ymthe.2022.08.012

Authors: Xuefeng Ai; Bingqian Yan; Nevin Witman; Yiqi Gong; Li Yang; Yao Tan; Ying Chen; Minglu Liu; Tingting Lu; Runjiao Luo; Huijing Wang; Kenneth R. Chien; Wei Wang; Wei Fu

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” unknown species cell and human PSC in “E8” for “1” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Transplantation of hPSC-derived pericyte-like cells promotes functional recovery in ischemic stroke mice

Mon, 01 Jan 0001 00:00:00 +0000

Transplantation of hPSC-derived pericyte-like cells promotes functional recovery in ischemic stroke mice

DOI: 10.1038/s41467-020-19042-y

Authors: Jiaqi Sun; Yinong Huang; Jin Gong; Jiancheng Wang; Yubao Fan; Jianye Cai; Yi Wang; Yuan Qiu; Yili Wei; Chuanfeng Xiong; Jierui Chen; Bin Wang; Yuanchen Ma; Lihua Huang; Xiaoyong Chen; Shuwei Zheng; Weijun Huang; Qiong Ke; Tao Wang; Xiaoping Li; Wei Zhang; Andy Peng Xiang; Weiqiang Li

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human cell in “mTeSR” for “1” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should be changed once in “1” days.

Transplantation of human induced pluripotent stem cell-derived neural crest cells for corneal endothelial regeneration

Mon, 01 Jan 0001 00:00:00 +0000

Transplantation of human induced pluripotent stem cell-derived neural crest cells for corneal endothelial regeneration

DOI: 10.1186/s13287-021-02267-z

Authors: Yajie Gong; Haoyun Duan; Xin Wang; Can Zhao; Wenjing Li; Chunxiao Dong; Zongyi Li; Qingjun Zhou

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “Matrigel” at “N/A” “N/A”.

Ideally, “PSC” should express “POU5F1”, “NANOG”, “SOX2” at the end of culture.

Transplantation of induced pluripotent stem cells-derived cardiomyocytes combined with modified Taohong Siwu decoction improved heart repair after myocardial infarction

Mon, 01 Jan 0001 00:00:00 +0000

Transplantation of induced pluripotent stem cells-derived cardiomyocytes combined with modified Taohong Siwu decoction improved heart repair after myocardial infarction

DOI: 10.1016/j.heliyon.2024.e26700

Authors: Zhi-rong Luo; Wan-ting Meng; Han Li; Yu Wang; Ya-chao Wang; Yue Zhao; Ping-ping Lu; Yuan Yuan; Wei Huang; Hai-dong Guo

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” human PSC in “unspecified medium”.

Ideally, “PSC” should express “POU5F1”, “NANOG” at the end of culture.

Transplantation of retinal pigment epithelium and photoreceptors generated concomitantly via small molecule-mediated differentiation rescues visual function in rodent models of retinal degeneration

Mon, 01 Jan 0001 00:00:00 +0000

Transplantation of retinal pigment epithelium and photoreceptors generated concomitantly via small molecule-mediated differentiation rescues visual function in rodent models of retinal degeneration

DOI: 10.1186/s13287-021-02134-x

Authors: Harshini Surendran; Swapna Nandakumar; Vijay Bhaskar Reddy K; Jonathan Stoddard; Varsha Mohan K; Pramod K. Upadhyay; Trevor J. McGill; Rajarshi Pal

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “mTeSR” for “1” days. For ECM, use “Matrigel” at “1” “%”. Medium should be changed once in “1” days.

Vaccination with vascular progenitor cells derived from induced pluripotent stem cells elicits antitumor immunity targeting vascular and tumor cells

Mon, 01 Jan 0001 00:00:00 +0000

Vaccination with vascular progenitor cells derived from induced pluripotent stem cells elicits antitumor immunity targeting vascular and tumor cells

DOI: 10.1007/s00262-014-1531-1

Authors: Shigeo Koido; Masaki Ito; Yukiko Sagawa; Masato Okamoto; Kazumi Hayashi; Eijiro Nagasaki; Shin Kan; Hideo Komita; Yuko Kamata; Sadamu Homma

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” mouse PSC in “DMEM”. For ECM, use “Gelatin” at “N/A” “N/A”. Medium should contain factors shown in the following table.

Vascular differentiation of multipotent spermatogonial stem cells derived from neonatal mouse testis

Mon, 01 Jan 0001 00:00:00 +0000

Vascular differentiation of multipotent spermatogonial stem cells derived from neonatal mouse testis

DOI: 10.3858/emm.2012.44.4.034

Authors: Ji-Eun Im; Sun-Hwa Song; Ji Yeon Kim; Koung Li Kim; Sang Hong Baek; Dong Ryul Lee; Wonhee Suh

Step 1: iPSC/ESC maintainance

day 0 ~ 2

culture “adherent” mouse mSSC in “unspecified medium” for “2” days. For ECM, use “Gelatin” at “N/A” “N/A”. Medium should be changed once in “2” days.

Ideally, “mSSC” should express “POU5F1”, “NANOG”, “PECAM1” at the end of culture.

Vascular endothelial cells derived from transgene-free pig induced pluripotent stem cells for vascular tissue engineering

Mon, 01 Jan 0001 00:00:00 +0000

Vascular endothelial cells derived from transgene-free pig induced pluripotent stem cells for vascular tissue engineering

DOI: 10.1016/j.actbio.2024.12.033

Authors: Luke Batty; Jinkyu Park; Lingfeng Qin; Muhammad Riaz; Yuyao Lin; Zhen Xu; Xuefei Gao; Xin Li; Colleen Lopez; Wei Zhang; Marie Hoareau; Meghan E. Fallon; Yan Huang; Hangqi Luo; Jiesi Luo; Séverine Ménoret; Peining Li; Zhenting Jiang; Peter Smith; David H. Sachs; George Tellides; Ignacio Anegon; Jordan S. Pober; Pentao Liu; Yibing Qyang

Vascular progenitors generated from tankyrase inhibitor-regulated naïve diabetic human iPSC potentiate efficient revascularization of ischemic retina

Mon, 01 Jan 0001 00:00:00 +0000

Vascular progenitors generated from tankyrase inhibitor-regulated naïve diabetic human iPSC potentiate efficient revascularization of ischemic retina

DOI: 10.1038/s41467-020-14764-5

Authors: Tea Soon Park; Ludovic Zimmerlin; Rebecca Evans-Moses; Justin Thomas; Jeffrey S. Huo; Riya Kanherkar; Alice He; Nensi Ruzgar; Rhonda Grebe; Imran Bhutto; Michael Barbato; Michael A. Koldobskiy; Gerard Lutty; Elias T. Zambidis

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” unknown species cell and human PSC in “DMEM/F12”. For ECM, use “Fibroblast” at “N/A” “N/A”. Medium should contain factors shown in the following table.

Vasculogenesis in kidney organoids upon transplantation

Mon, 01 Jan 0001 00:00:00 +0000

Vasculogenesis in kidney organoids upon transplantation

DOI: 10.1038/s41536-022-00237-4

Authors: Marije Koning; Sébastien J. Dumas; M. Cristina Avramut; Roman I. Koning; Elda Meta; Ellen Lievers; Loes E. Wiersma; Mila Borri; Xue Liang; Lin Xie; Ping Liu; Fang Chen; Lin Lin; Yonglun Luo; Jaap Mulder; H. Siebe Spijker; Thierry Jaffredo; Bernard M. van den Berg; Peter Carmeliet; Cathelijne W. van den Berg; Ton J. Rabelink

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

VEGF – Supplemented extracellular matrix is sufficient to induce endothelial differentiation of human iPSC

Mon, 01 Jan 0001 00:00:00 +0000

VEGF – Supplemented extracellular matrix is sufficient to induce endothelial differentiation of human iPSC

DOI: 10.1016/j.biomaterials.2019.119283

Authors: Imran Ullah; Raed Abu-Dawud; Jonas Felix Busch; Anja Rabien; Bettina Erguen; Iris Fischer; Petra Reinke; Andreas Kurtz

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” human PSC in “E8”. For ECM, use “kidney dECM” at “N/A” “N/A”. Medium should contain factors shown in the following table.

VEGF, FGF2, and BMP4 regulate transitions of mesoderm to endothelium and blood cells in a human model of yolk sac hematopoiesis

Mon, 01 Jan 0001 00:00:00 +0000

VEGF, FGF2, and BMP4 regulate transitions of mesoderm to endothelium and blood cells in a human model of yolk sac hematopoiesis

DOI: 10.1016/j.exphem.2021.08.006

Authors: Freya F. Bruveris; Elizabeth S. Ng; Edouard G. Stanley; Andrew G. Elefanty

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human ESC in “unspecified medium”. For ECM, use “Fibroblast” at “N/A” “N/A”.

Ideally, “ESC” should be obtained at the end of culture.

Versatile synthetic alternatives to Matrigel for vascular toxicity screening and stem cell expansion

Mon, 01 Jan 0001 00:00:00 +0000

Versatile synthetic alternatives to Matrigel for vascular toxicity screening and stem cell expansion

DOI: 10.1038/s41551-017-0096

Authors: Eric H. Nguyen; William T. Daly; Ngoc Nhi T. Le; Mitra Farnoodian; David G. Belair; Michael P. Schwartz; Connie S. Lebakken; Gene E. Ananiev; Mohammad Ali Saghiri; Thomas B. Knudsen; Nader Sheibani; William L. Murphy

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human ESC in “E8” for “1” days. For ECM, use “hydrogel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

VGLL4 promotes vascular endothelium specification via TEAD1 in the vascular organoids and human pluripotent stem cells-derived endothelium model

Mon, 01 Jan 0001 00:00:00 +0000

VGLL4 promotes vascular endothelium specification via TEAD1 in the vascular organoids and human pluripotent stem cells-derived endothelium model

DOI: 10.1007/s00018-023-04858-w

Authors: Yingyi Quan; Minjie Hu; Jiang Jiang; Peifeng Jin; Junming Fan; Ming Li; Xiaofang Fan; Yongsheng Gong; Yang Yang; Yongyu Wang

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human ESC in “Nuwacell hiPSC/hESC medium-ncEpic” for “1” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Vitamin C–dependent lysine demethylase 6 (KDM6)-mediated demethylation promotes a chromatin state that supports the endothelial-to-hematopoietic transition

Mon, 01 Jan 0001 00:00:00 +0000

Vitamin C–dependent lysine demethylase 6 (KDM6)-mediated demethylation promotes a chromatin state that supports the endothelial-to-hematopoietic transition

DOI: 10.1074/jbc.RA119.009757

Authors: Tian Zhang; Ke Huang; Yanling Zhu; Tianyu Wang; Yongli Shan; Bing Long; Yuhang Li; Qianyu Chen; Pengtao Wang; Shaoyang Zhao; Dongwei Li; Chuman Wu; Baoqiang Kang; Jiaming Gu; Yuchan Mai; Qing Wang; Jinbing Li; Yanqi Zhang; Zechuan Liang; Lin Guo; Fang Wu; Shuquan Su; Junwei Wang; Minghui Gao; Xiaofen Zhong; Baojian Liao; Jiekai Chen; Xiao Zhang; Xiaodong Shu; Duanqing Pei; Jinfu Nie; Guangjin Pan

VSELs Maintain their Pluripotency and Competence to Differentiate after Enhanced Ex Vivo Expansion

Mon, 01 Jan 0001 00:00:00 +0000

VSELs Maintain their Pluripotency and Competence to Differentiate after Enhanced Ex Vivo Expansion

DOI: 10.1007/s12015-018-9821-1

Authors: Rachid Lahlil; Maurice Scrofani; Romain Barbet; Céline Tancredi; Anne Aries; Philippe Hénon

Step 1: mesoderm 1

day 0 ~ 2

culture “adherent” unknown species cell and human VSEL in “unspecified medium” for “2” days. For ECM, use “fibronectin/gelatin” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Wnt Inhibition Facilitates RNA-Mediated Reprogramming of Human Somatic Cells to Naive Pluripotency

Mon, 01 Jan 0001 00:00:00 +0000

Wnt Inhibition Facilitates RNA-Mediated Reprogramming of Human Somatic Cells to Naive Pluripotency

DOI: 10.1016/j.stemcr.2019.10.009

Authors: Nicholas Bredenkamp; Jian Yang; James Clarke; Giuliano Giuseppe Stirparo; Ferdinand von Meyenn; Sabine Dietmann; Duncan Baker; Rosalind Drummond; Yongming Ren; Dongwei Li; Chuman Wu; Maria Rostovskaya; Sarah Eminli-Meissner; Austin Smith; Ge Guo

Step 1: iPSC/ESC maintainance

day 0 ~ 10

culture “adherent” human PSC in “N2B27” for “10” days. Medium should contain factors shown in the following table, and should be changed once in “10” days.

Wnt signaling controls the specification of definitive and primitive hematopoiesis from human pluripotent stem cells

Mon, 01 Jan 0001 00:00:00 +0000

Wnt signaling controls the specification of definitive and primitive hematopoiesis from human pluripotent stem cells

DOI: 10.1038/nbt.2915

Authors: Christopher M Sturgeon; Andrea Ditadi; Geneve Awong; Marion Kennedy; Gordon Keller

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “hESC medium” for “1” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should be changed once in “1” days.

Step 2: Primitive streak 1

day 1 ~ 4

Wnt/β-Catenin Stimulation and Laminins Support Cardiovascular Cell Progenitor Expansion from Human Fetal Cardiac Mesenchymal Stromal Cells

Mon, 01 Jan 0001 00:00:00 +0000

Wnt/β-Catenin Stimulation and Laminins Support Cardiovascular Cell Progenitor Expansion from Human Fetal Cardiac Mesenchymal Stromal Cells

DOI: 10.1016/j.stemcr.2016.02.014

Authors: Agneta Månsson-Broberg; Sergey Rodin; Ivana Bulatovic; Cristián Ibarra; Marie Löfling; Rami Genead; Eva Wärdell; Ulrika Felldin; Carl Granath; Evren Alici; Katarina Le Blanc; C.I. Edvard Smith; Alena Salašová; Magnus Westgren; Erik Sundström; Per Uhlén; Ernest Arenas; Christer Sylvén; Karl Tryggvason; Matthias Corbascio; Oscar E. Simonson; Cecilia Österholm; Karl-Henrik Grinnemo

Step 1: endothelial 1

day 0 ~ 21

ZEB2 and MEIS1 independently contribute to hematopoiesis via early hematopoietic enhancer activation

Mon, 01 Jan 0001 00:00:00 +0000

ZEB2 and MEIS1 independently contribute to hematopoiesis via early hematopoietic enhancer activation

DOI: 10.1016/j.isci.2023.107893

Authors: Yohko Kitagawa; Akihiro Ikenaka; Ryohichi Sugimura; Akira Niwa; Megumu K. Saito

Step 1: iPSC/ESC maintainance

day 0 ~ 3

culture “adherent” human PSC in “mTeSR” for “3” days. For ECM, use “Laminin” at “N/A” “N/A”. Medium should be changed once in “3” days.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: Primitive streak 1

day 3 ~ 5